DAFTAR PUSTAKA. Cahyadi W Kedelai Khasiat dan Teknologi. Jakarta: Bumi Aksara

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1 48 DAFTAR PUSTAKA Anderson JJ, Ambrose WW, Garner SC Orally Dosed Genestein from Soy and Prevention of Concellous Bone Loss in Two Ovariektomized Rat Models. J. Nutr. 25: 799S (Abstract). Anonim Soy isoflavone aglycones have estrogenic effects on mouse reproductiveetracts. Complementary_and_Alternative_Medicine asp.[31 Maret 2008]. Astuti S Pengaruh Tepung Kedelai dan Tempe Dalam Ransum Terhadap Fertilitas Tikus Percobaan [Tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor. Arjmandi BH, Alekel L, Hollis BW, Amin D, Stacewics-Sapuntzakis M, Guo P, Kukreja SC Diatery Soybean Protein Prevents Bone Loss in Ovariectomized Rat Model of Osteoporosis. J. Nutr. 126: Beard APM, Hunter G, Laming GE Quantitative control of oxytosininduced estradiol PGF2α release by progesterone and estradiol in ewes. J Reprod And fert. 100 : Cahyadi W Kedelai Khasiat dan Teknologi. Jakarta: Bumi Aksara Casanova M Developmental effect of dietary phytoestrogens in Sprague- Dawley rats and interactions of genistein and daidzein with rat estrogen receptors alpha and beta in vitro. Toxicol Sci 51:2. Abstract.html [1 Mei 2007]. Coward L, Barnes NC, Setchell KDR, Barnes S Genestein and Daidzein and Their β-glucoside Conjugates: Antitumor Isoflavone in Soybean Foods from Amerika and Asia Diets. J Agric Food Chem. 41 : [Deptan RI] Departemen Pertanian Republik Indonesia Peningkatan Produksi Kedelai (Glycine max). Jakarta: Departemen Pertanian Republik Indonesia Direktorat Jenderal Bina Produksi Tanaman PanganDDirektoratKKacang-kacanganddanuUmbi-umbian. [1 Mei 2007]. Gadjahnata KHO Biologi Kedokteran I. Bogor: IPB Press Garvita RV Efektivitas Ekstrak Kedelai pada Pra-Kebuntingan (5, 10, 15 Hari) Tikus Untuk Meningkatkan Profil Reproduksi [Tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor.

2 49 Guyton AC Fisiologi Kedokteran bagian III. Ed ke-7. Jakarta: Penerbit Buku Kedokteran EGC. Guyton AC Fisiologi Manusia dan Mekanisme Penyakit (Human Physiology and Mechanism of disease). Terjemahan. Ed ke-3. Jakarta: Buku Kedokteran EGC. Granner DK Hormon Kelamin. Dalam: Biokimia (Harper s Review of Biochemestry). Diterjemahkan oleh Darmawan I. Jakarta: EGC. Hafez ESE, Jainudeen MR, Rosnina Y Hormones, Growth Factors and Reproduction. Di dalam: Reproduction in Farm Animals. Ed-3.Lippincott Williams & Wilkins. Philadelphia.hlm Hunter RHF Fisiologi dan Teknologi Reproduksi Hewan Betina Domestik. Alih bahasa D.K. Harya Putra. Bandung : Institut Teknologi Bandung Press. Hardjopranjoto S Ilmu Kemajiran pada Ternak. Surabaya. Airlangga University Press. Heinnermen J Peran isoflavon untuk kesehatan reproduksi wanita. Cermin Dunia Kedokteran 139. [IHME] Indonesian Hospital Medical Equipment Isoflavon, Makanan Ajaib.Jakarta:PPPusatDDataaa&IInformasiPPERSI.hhttp:// o.id/images/news/content/tempe.jpg [11 April 2008]. Jeferson Assessing estrogenic activity of phytochemicals using transcriptional activation and immature mouse uterotropic responses. J chromatogr B Analyt TechnolBBiomedLLifeSSci7777:179. Jones MEE, Thorburn AW, Britt KI, Hewitt KN, Wreford NG, Proietto J, Oz OK, Leury BJ, Robertson KM, Yao S, Simpson ER Aromatase-Deficient (ArKO) Mice have Phenotype of Increased Adiposity. Proc. Natl. Acad. Sci. USA. 97(23): Koswara S Teknologi Pengolahan Kedelai Menjadikan Makanan Bermutu. Jakarta: Pustaka Sinar Harapan. Koswara S Teknologi Pengolahan Kedelai Menjadikan Makanan Bermutu. Jakarta: Pustaka Sinar Harapan. Kuiper GGJM, Carlsson B, Grandien K, Enmark E, Haggblod J, Nilsson S, Gustafsson JA Comparison of the Ligand Binding Specificity and Transcript Tissue Distribution of Estrogen Receptor α and β. J. Endocrinology. 138:

3 50 Malole MBM, Pramono CSU Penggunaan Hewan-hewan Percobaan di Laboratorium. Departemen Pendidikan dan Kebudayaan, Dirjen Pendidikan Tinggi, Pusat Antar Universitas Bioteknologi. Bogor: IPB Press. Mattjik AA, Sumertajaya IM Perancangan Percobaan Dengan Aplikasi SAS dan MINITAB. Ed ke-3. Bogor: IPB Press. Murata K, Ikehata H, Miyamoto T Studies on the Ntritional Value of Tempeh. J Food Sci. 32(5): Nakajima N, Nozak N, Ishihara N, Ishikawa A, Tsuji H Analysis of Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a New Isoflavone- Enriched Tempeh. Journal of Bioscience and Bioengineering vol.100.no.6, DOL: /jbb Nalbandov AV Fisiologi Reproduksi pada Mamalia dan Unggas. Alih bahasa S. Keman. Jakarta: Universitas Indonesia Press. Nurdin SU Pengaruh Pemberian Tepung Tahu dan Tempe Kedelai dalam Ransum terhadap Massa dan Densitas Tulang Tikus Betina Ovariektomi.[Thesis].Bogor: Program Pasca Sarjana. Pakasi LS Monopouse: Masalah dan Penanggulangannya. Jakarta: Fakultas Kedokteran Universitas Indonesia. PawiroharsonoSS Prospek dan Manfaat Isoflavon untuk Kesehatan. Jakarta: Direktorat Teknologi Bioindustri, Badan Pengkajian dan PenerapanTTeknologi.hhttp:// 001/indexisi.asp?fi:epus [1 Mei 2007] Persky VW, Turyk ME,Lingt W,Freels S,Barnes S Effect of soy protein on endogenous in postmenopausal women. Am J. Clin Nutr.75: Pineda MH Female reproductive system. London: Lea & Febiger. Qiju W Purification and antioxidant activities of soybean isoflavon. A Thesis Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partil filfillment of the requirements for the degree of Master of Science in The Department of Food Science B.S.,Zhejiang University. Santell RC, Chang YC, Nair MG, Helferich WG Dietary Genestein Exerts Estrogenic Effects Upon the Uterus, Mammary Gland, and the Hyphothalamic/Pituitary Axis in Rats. J. Nutr. 127:

4 51 Setchell KDR, Cassidy A Dietary isoflavon: Biological effect and relevance to human health. Symposium on Phytoestrogenicals: Biochemistry and Physiology. J Nutr.129:758S-767S. Shurtleff WA, Aoyagi The Book of Tempeh. New York: Harper and Row. Smith JB, Mangkoewidjojo S Pemeliharaan, pembiakan dan penggunaan hewan percobaan di daerah tropis. Jakarta: U Press. Tanu I Farmakologi dan Terapi. Ed-4. Jakarta: Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia. Toelihere MR Fisiologi reproduksi pada ternak. Bandung: Angkasa. Turner CD, Bagnara JT Endokrinologi Umum. Ed ke-6. Yogyakarta: Air Langga University Press. Whitten PL, Patisaul HB Cross-species and interassay comparisons of phytoestrogenaction.eenvironhhealthpperspect.hhttp:// rvice.co.nz/htm [1 Mei 2007]. [Wikipedia]WWikipedia BBrownRRat.hhttp://en.wikipedia.org/wiki/Imag e:rattus norvegicus_1.jpg [1 Mei 2007]. [Wikipedia]WWikipedia SSoybean. bean.usda.jpg [1 Mei 2007] Winarsi H Isoflavon berbagai sumber, sifat, dan manfatnya pada penyakit degeneratif. Yogyakarta: Gadjah mada University Press. Wodzicka-Tomaszewska M, Sutama IK, Putu IG, Chaniago Reproduksi, Tingkah Laku dan Produksi Ternak di Indonesia. Jakarta: Gramedia. Wuryani W Isoflavone in Tempe. Asean Food Journal. 3(10): Yulianto KKedelai,BBahanPPanganPPenyayangTTulang.hhttp://www. sinarhar pan.coid/iptek/kesehatan/2003/1024/kes1.html.[16 Mei 2007] Zhang Y, Song TT, Cunnick JE, Murphy PA, Hendric HS Daidzein and genistein glucuronides in vitro are weakly estrogenic and active human natural killer cell at nutritionally relevant concentrations. J. Nutr.129:

5 52 Lampiran 1. Proses pembuatan tempe KEDELAI Pembersihan Pencucian + Perendaman Perebusan (100 0 C, 30 Menit) Perendaman dengan air rebusan (22 Jam) Pengupasan kulit Perebusan dalam air asam (45 menit) Pendinginan (suhu kamar) Peragian (2 gr/kg kedelai) Pembungkusan dengan plastik Inkubasi (suhu 31 0 C, RH 78-85%; 24 jam) TEMPE

6 53 Lampiran 2. Proses pembuatan tepung kedelai Kedelai Penggilingan (diblender) Pengeringan (oven), suhu 45 0 C, kadar air 10% Penepungan (disk mill) Pengayakan (60 mesh) Tepung Kedelai

7 54 Lampiran 3. Proses pembuatan tepung tempe Tempe Mentah Pengirisan Tempe (1 x 2 x 0,5 cm 3 ) Penggilingan (diblender) Pengeringan (oven), suhu 45 0 C, kadar air 10% Penepungan (disk mill) Pengayakan (60 mesh) Tepung Tempe

8 55 Lampiran 4. Prosedur analisis senyawa isoflavon. Ekstraksi Tepung Kedelai dan Tepung Tempe Tepung kedelai dan tepung tempe dikeringkan selama 4 jam. Selanjutnya diekstrak dengan larutan methanol 99,8% sebanyak 2 kali, masing-masing 100 ml dengan menggunakan pompa vakum yang sudah dilapisi kertas kering, sehingga metanol yang digunakan untuk mengekstrak sebanyak 200 ml. Senyawa isoflavon termasuk senyawa polar sehingga mudah larut dengan larutan polar seperti alkohol atau metanol. Ekstraksi dilakukan 2 kali agar senyawa isoflavon dalam tepung kedelai dan tepung tempe terekstrak secara keseluruhan. Ekstrak yang diperoleh disimpan dalam ruang dingin kurang lebih 0 0 C selama satu malam untuk menggumpalkan lemaknya. Lemak yang ada dipisahkan melalui penyaringan dengan pompa vakum yang dilapisi kertas saring. Filtrat yang diperoleh selanjutnya diuapkan dengan rotavapor pada suhu 40 0 C. Ekstrak yang telah kering dilarutkan dalam 10 ml metanol absolute (p.a), kemudian disentrifuse pada kecepatan 4000 rpm selama 5 menit dengan tujuan untuk mendapatkan filtrate yang bening dan memisahkan endapan yang terbentuk. Pemurnian Isoflavon Filtrat yang terbentuk diambil 1.5 ml dan dimurnikan dalam kolom kromatografi volume 12 ml yang berisi MN-Poliamida CC6 dengan ukuran partikel mm. Sebelum filtrate dituangkan ke dalam kolom, MN- Poliamida CC6 direndam semalam dalam kromatografi dengan larutan metanol 25%. Elusi dalam kolom dilakukan secara bertahap dengan metanol 25%, 50%, 70%, masing-masing 50 ml. Eluen yang didapat dari elusi metanol 70% (fraksi 70%) ditampung dan dikeringkan dengan rotavapor pada suhu 40 0 C sampai kering. Residu (endapan kering yang diperoleh) dilarutkan dalam 1 ml metanol absolut dan disentrifuse pada 4000 rpm selama 5 menit untuk memisahkan endapan yang ada, kemudian disaring. Filtrat yang bening siap untuk dianalisa dengan menggunakan HPLC.

9 56 Identifikasi Isoflavon Analisis kuantitatif dilakukan dengan kromatografi cairan tekanan tinggi (HPLC). HPLC yang digunakan berada pada kondisi sebagai berikut: Volume sampel : 40 µl Kolom : Li chrosorb RP-18 (250 x 4 mm, 5 µm) Eluen : Asam asetat 3% (pelarut A); Acetonitril (pelarut B) Gradien : 20% dalam (A + B) sampai 60% (A+B) selama 35 menit. Detektor : UV 261 nm Kecepatan alir : 0.8 ml/menit Suhu : 35 0 C

10 57 50 g Sampel dikeringkan pada suhu 40 0 c selama 5 jam Diektrasksi dengan metanol absolut dua kali masing-masing 100 ml, disaring dengan dengan pompa vakum Ekstrak disimpan dalam ruang pendingin 0 0 C selama semalam untuk menggumpalkan lemaknya Lemak dipisahkan dengan pompa vakum Filtrat jernih diuapkan dengan rotavapor pada suhu 40 0 C Residu dilarutkan dalam 10 ml metanol absolut Disentrifuse pada kecepatan 4000 rpm selama 5 menit Diambil 1.5 ml filtrat untuk dipurifukasi dalam kolom kromatografi yang berisi MN-Poliamida CC6 Dielusi bertahap dengan metanol 25%, 50% dan 70%, masing-masing 50 ml Hasil elusi dengan 70% metanol dikeringkan dengan rotavapor pada suhu 40 0 C Residu dilarutkan dalam ml metanol grade HPLC Disentrifusi pada 4000 rpm selama 5 menit Diambil 40 µl untuk diinjeksi pada HPLC Bagan Alir Analisis Kuantitatif Senyawa Isoflavon

11 58 Lampiran 5. Contoh perhitungan kandungan senyawa isoflavon. NP 289 (Tepung Kedelai) ppm NP 290 (Tepung Tempe) ppm

12 Lampiran 6. Kromatogram HPLC Standar Senyawa Isoflavon. 59

13 Lampiran 7. Kromatogram HPLC Tepung Kedelai. 60

14 Lampiran 8. Kromatogram HPLC Tepung Tempe 61

15 62 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU I hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu I (PPBB MI) Model Error Corrected Total R-Square Coeff Var Root MSE PPBB MI Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for PPBB MI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV A A OV Tp A A Kontrol A A OV Kd

16 63 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU II hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu II (PPBB MII) Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE PPBB MII Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for PPBB MII NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV A A OV Kd B OV Tp C Kontrol

17 64 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU III hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu III (PPBB MIII) Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE PPBB MIII Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for PPBB MIII NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV B OV Tp B B Kontrol C OV Kd

18 65 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU IV hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu IV (PPBB MIV) Model Error Corrected Total R-Square Coeff Var Root MSE PPBB MIV Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for PPBB MIV NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV A A OV Tp B OV Kd B B Kontrol

19 66 ANALISIS STATISTIK PERSENTASE KENAIKAN BERAT BADAN TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase kenaikan berat badan Model Error Corrected Total R-Square Coeff Var Root MSE persentase kenaikan berat badan Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for persentase kenaikan berat badan NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV B OV Tp B B Kontrol C OV Kd

20 67 ANALISIS STATISTIK BERAT BADAN AKHIR TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 P1 P2 P3 P4 Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan akhir Model Error Corrected Total R-Square Coeff Var Root MSE berat badan akhir Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for berat badan akhir NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A P1 A A P4 B P3 B B P2

21 68 ANALISIS STATISTIK BERAT UTERUS TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd Ov Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat uterus Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE berat uterus Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for berat uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan A Non OV A A OV Tp B OV Kd C Kontrol

22 69 ANALISIS STATISTIK BERAT UTERUS PER BERAT BADAN TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 Kontrol Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat uterus/berat badan tikus Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE berat uterus/berat badan tikus Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for berat uterus/berat badan tikus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV A A OV Tp B OV Kd C Kontrol

23 70 UJI KORELASI DAN REGRESI PERSENTASE KENAIKAN BERAT BADAN TERHADAP RASIO BERAT UTERUS PER BERAT BADAN TIKUS The SAS System The CORR Procedure 2 Variables: x y Simple Statistics Variable N Mean Std Dev Sum Minimum Maximum x y Pearson Correlation Coefficients, N = 4 Prob > r under H0: Rho=0 x y x y The GLM Procedure Number of Observations Read 4 Number of Observations Used 4 The GLM Procedure Dependent Variable: y Model Error Corrected Total R-Square Coeff Var Root MSE y Mean Source DF Type I SS Mean Square F Value Pr > F x Source DF Type III SS Mean Square F Value Pr > F x Standard Parameter Estimate Error t Value Pr > t Intercept x

24 71 ANALISIS STATISTIK PANJANG UTERUS KIRI TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non ov Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: panjang uterus kiri Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE panjang uterus kiri Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for panjang uterus kiri NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV B OV Tp B C B OV Kd C C Kontrol

25 72 ANALISIS STATISTIK PANJANG UTERUS KANAN TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: panjang uterus kanan Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE panjang uterus kanan Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for panjang uterus kanan NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV B OV Tp B C B OV Kd C C Kontrol

26 73 ANALISIS STATISTIK TOTAL PANJANG UTERUS TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: total panjang uterus Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE totalpanjanguterus Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for total panjang uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non Ov B OV Tp C OV Kd D Kontrol

27 74 ANALISIS STATISTIK BERAT BASAH BEBAS LEMAK UTERUS TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat basah bebas lemak uterus Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE berat basah bebas lemak uterus Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for berat basah bebas lemak uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan A Non Ov A A OV Tp B OV Kd C Kontrol

28 75 ANALISIS STATISTIL BERAT KERING BEBAS LEMAK UTERUS TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat kering bebas lemak uterus Model <.0001 Error Corrected Total R-Square Coeff Var Root MSE berat kering bebas lemaku terus Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan <.0001 Duncan's Multiple Range Test for berat kering bebas lemaku terus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan A Non OV B OV Tp C OV Kd D Kontrol

29 76 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU I hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu I Model Error Corrected Total R-Square Coeff Var Root MSE berat badan minggu I Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for berat badan minggu I NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A OV Kd A A OV Tp B Kontrol B B Non OV

30 77 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU II hasil penelitian Class Level Information Class Levels Values perlakuan 4 Nn OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu II Model Error Corrected Total R-Square Coeff Var Root MSE berat badan minggu II Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for berat badan minggu II NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A OV Kd B Non OV B C B OV Tp C C Kontrol

31 78 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU III hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu III Model Error Corrected Total R-Square Coeff Var Root MSE berat badan minggu III Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for berat badan minggu III NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A OV Tp A A Non OV A B A OV Kd B B OV Tp

32 79 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU III hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu IV Model Error Corrected Total R-Square Coeff Var Root MSE berat badan minggu IV Mean Source DF Anova SS Mean Square F Value Pr > F perlakuan Duncan's Multiple Range Test for berat badan minggu IV NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square Number of Means Critical Range Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A Non OV A A OV Tp B OV Kd B B Kontrol

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