7. LAMPIRAN Lampiran 1. Kurva Standar Protein Larutan Bardfrod Commasive blue ditimbang sebanyak 0,01 gram kemudian dilarutkan ke dalam 5 ml etanol 95% dan ditambah dengan 10 ml asam fosfor. Larutan selanjutnya disaring dengan menggunakan kertas saring dan disimpan dalam botol gelap pada suhu rendah. Sebelum digunakan, larutan harus diencerkan terlebih dahulu sebanyak lima kali (Bollag & Stuart, 1991) Pembuatan Kurva Standar Bovine Serum Albumin (BSA) ditimbang sebanyak 100 mg kemudian dilarutkan ke dalam 100 ml ddh 2 O dan larutan tersebut dinamakan larutan stok BSA 1 mg/ml. Selanjutnya, larutan stok BSA dibuat seri pengenceran larutan standar dengan konsentrasi 0 mg/ml, 0,1 mg/ml, 0,2 mg/ml, 0,3 mg/ml, 0,4 mg/ml, 0,5 mg/ml, 0,6 mg/ml, 0,7 mg/ml, 0,8 mg/ml, 0,9 mg/ml, dan 1 mg/ml. Dari masing-masing konsentrasi larutan standar diambil sebanyak 0,1 ml, lalu ditambahkan 5 ml larutan Bradford. Setelah itu, larutan didiamkan selama 5 menit, dan diukur absorbansinya pada panjang gelombang 595 nm (Bradford, 1976). Gambar 19. Kurva Standar Protein 49
50 Lampiran 2. Komposisi Reagen Elektroforesis Protein Larutan Stok (Stock Solutions) Bahan-bahan yang digunakan dalam pembuatan larutan stok adalah 2M Tris-HCl (ph 8,8) 100 ml, 1 Tris-HCl (ph 6,8) 100 ml, 10% SDS 100 ml, 50% gliserol 100 ml, dan 1% bromophenol blue 10 ml. Larutan Kerja (Working Solutions) Nama Larutan Larutan A (Acrylamide Stock Solution), 100 ml 30% acrylamide, 0,8% bisacrylamide Larutan B (4x Separating Gel Buffer), 100 ml Komposisi 29,2 gram acrylamide 0,8 gram bis-acrylamide 75 ml 2M Tris-HCl (ph 8,8) --- 1,5 M 4 ml 10% SDS --- 0,4 % 21 ml ddh 2 O Larutan C 50 ml 1M Tris-HCl (ph 6,8) --- 0,5 M (4x Stacking Gel Buffer), 100 ml 4 ml 10% SDS --- 0,4 % 46 ml ddh 2 O 10% Ammonium Persulphate 0,5 gram APS (APS) 5 ml DDH 2 O Buffer Elektroforesis 3 gr Tris --- 25Mm 14,4 gram glycine --- 192Mm 1 gram SDS --- 0,1% ddh 2 O sampai 1 liter Sampel Buffer 3,028 gram Tris 0,25 ml larutan C 0,4 ml 10% SDS 25 ml gliserol 0,2 mg bromophenol blue 0,031 gram Dithiothreitol (DTT) Larutan 12,5% Separating Gel Buffer (dalam 10 ml) Larutan Jumlah Larutan acrylamide 4,2 ml 4x Separating gel buffer 2,5 ml 10% SDS 100 µl ddh 2 O 3,2 ml 10% Ammonium Persulphate (APS) 50 µl TEMED 3,4 µl
51 Larutan 4x Stacking Gel Buffer (dalam 10 ml) Larutan Jumlah Larutan acrylamide 2,66 ml 4x stacking gel buffer 2,5 ml 10% SDS 0,1 ml ddh 2 O 6 ml 10% Ammonium Persulphate (APS) 50 µl TEMED 5 µl
52 Lampiran 3. Kurva Standar BSA (Bovine Serum Albumin) Low Moleculer Weight Gambar 20. Kurva Standar BSA (Bovine Serum Albumin) Low Moleculer Weight
53 Lampiran 4. Hasil Pengolahan SPSS Tingkat Kekerasan (Hardness) pada Adonan Tepung Terigu Duncan a Adonan Tpeung Terigu Kontrol Adonan Tepung Terigu + Jahe 1,5% Adonan Tepung Terigu + Jahe 3% Hardness f or alpha =.05 6 183,0533 6 220,4083 6 310,7017
54 Lampiran 5. Hasil Pengolahan SPSS Karakteristik Fisik Roti Kukus a. Tingkat Kekerasan (Hardness) Roti Kukus Jahe 0% Hardness Roti Kukus Jahe 0% 6 115,9317 6 176,0700 6 370,4967 The error term is Mean Square(Error) = 40,777. Hardness 6 143,8850 6 217,5817 6 712,6117 The error term is Mean Square(Error) = 261,490. Hardness 6 168,1267 6 265,6117 6 899,9483 The error term is Mean Square(Error) = 57,050.
55 Hardness 6 176,0700 6 217,5817 6 265,6117 The error term is Mean Square(Error) = 56,683. Proofing Hardness Proofing 6 115,9317 6 143,8850 6 168,1267 The error term is Mean Square(Error) = 22,232. Hardness 6 370,4967 6 712,6117 6 899,9483 The error term is Mean Square(Error) = 280,403.
56 b. Warna L* Duncan a L for alpha =.05 6 75,1567 6 76,3233 6 84,1850 a* Duncan a a for alpha =.05 6-2,7733 6-2,3667 6 -,8083 b* Duncan a b for alpha =.05 6 20,4600 6 24,3767 6 27,7800
57 c. Volume Pengembangan Roti Kukus Jahe 0% Volume 6 31,2017 6 55,3967 6 81,6717 The error term is Mean Square(Error) =,792. Volume 6 35,4583 6 47,7350 6 74,2233 The error term is Mean Square(Error) =,761. Volume 6 39,4717 6 51,8533 6 77,4100 The error term is Mean Square(Error) =,894.
58 Volume 6 31,2017 6 35,4583 6 39,4717 The error term is Mean Square(Error) =,778. Proofing Volume 6 47,7350 6 51,8533 6 55,3967 The error term is Mean Square(Error) =,801. Volume 6 74,2233 6 77,4100 6 81,6717 The error term is Mean Square(Error) =,868.
59 Lampiran 6. Hasil Pengolahan SPSS Aktivitas Antioksidan pada Roti Kukus Roti Kukus Jahe 0% Antioksidan 6 2,9567 6 8,0750 6 11,4750 The error term is Mean Square(Error) =,877. Antioksidan 6 9,3483 6 13,8067 6 21,6717 The error term is Mean Square(Error) = 3,089. Antioksidan 6 18,7217 6 26,3800 6 34,1617 The error term is Mean Square(Error) = 1,153.
60 Antioksidan 6 11,4750 6 21,6717 6 34,1617 The error term is Mean Square(Error) = 1,327. Proofing Antioksidan 6 8,0750 6 13,8067 6 26,3800 The error term is Mean Square(Error) = 2,604. Antioksidan 6 2,9567 6 9,3483 6 18,7217 The error term is Mean Square(Error) = 1,188.