Lampiran 2 : Analisis Jumlah Kuadrat Utama. 1. Faktor Koreksi ( Fk ) = = = 2. Jumlah Kuadrat Total = = = = = ,105 J k total = 3811,895

Transkripsi

1 Lampiran 2 : Analisis Jumlah Kuadrat Utama 1. Faktor Koreksi ( Fk ) 2. Jumlah Kuadrat Total ,105 J k total 3811, Jumlah Kuadrat Kelompok 0,106

2 4. Jumlah Kuadart Perlakuan (Pestisida) 3413, Jumlah Kuadrat Galat ( JkG ) 398,501

3 Lampiran : 5 Certificate No CLIENT REPORT OF ANALYSIS RECEIVED ON : March 23, 2012 : BALAI BESAR KARANTINA PERTANIAN BELAWAN TEST REQUIRED : Azadirachtin A (Aza A), Azadirachtin B (Aza B) Nimbin (NIM), Salannin (SAL) DESCRIPTION OF SAMPLE : Sample was submitted by client Form : Manggis S Nimba 30 % Description : Susana Bangun March 24, 2012 METHOD & EQUIPMENT : Homogenized sample was extracted by liquid-liquid extraction (LLE) method. Analysis performed by HPLC with Triple Quadrupole Tandem Mass Spectrometry detector (LC-MS/MS). Qualitative analyses were conducted by monitoring Ion Ratio of 2 MRM pairs for each compound. Quantitative determinations were calculated by standard addition using single point matrix matched calibration at RL. TEST RESULTS : Measurand RL ppm (mg/kg) Result Azadirachtin A 0.20 ND Azadirachtin B 0.20 ND Nimbin 0.20 ND Salannin 0.20 ND Note : ND : Not Detected below RL LoQ : Limit of Quantitation. Lowest level for which it has been demonstrated that criteria for accuracy and precision have been met, measured once during method validation. RL : Reporting Limit practical LoQ. RL is measured every analysis batch. Precision and accuracy of the analysis batch had been checked and fulfilled the declared Laboratory Quality Control Criteria (x.xx) : (. ) as character indicates decimal notation KJU KM Analysis batch : #OA-PES Laboratorium Director This result related to the samples submitted only. Suwidji Wongso, Ph.D.

4 Lampiran : 6 Certificate No CLIENT REPORT OF ANALYSIS RECEIVED ON : March 23, 2012 : BALAI BESAR KARANTINA PERTANIAN BELAWAN TEST REQUIRED : Azadirachtin A (Aza A), Azadirachtin B (Aza B) Nimbin (NIM), Salannin (SAL) DESCRIPTION OF SAMPLE : Sample was submitted by client Form : Manggis M Nimba 2,5 % Description : Susana Bangun March 24, 2012 METHOD & EQUIPMENT : Homogenized sample was extracted by liquid-liquid extraction (LLE) method. Analysis performed by HPLC with Triple Quadrupole Tandem Mass Spectrometry detector (LC-MS/MS). Qualitative analyses were conducted by monitoring Ion Ratio of 2 MRM pairs for each compound. Quantitative determinations were calculated by standard addition using single point matrix matched calibration at RL. TEST RESULTS : ppm (mg/kg) Measurand RL Result Azadirachtin A 0.20 ND Azadirachtin B 0.20 ND Nimbin 0.20 ND Salannin Note : ND : Not Detected below RL LoQ : Limit of Quantitation. Lowest level for which it has been demonstrated that criteria for accuracy and precision have been met, measured once during method validation. RL : Reporting Limit practical LoQ. RL is measured every analysis batch. Precision and accuracy of the analysis batch had been checked and fulfilled the declared Laboratory Quality Control Criteria (x.xx) : (. ) as character indicates decimal notation KJU KM Analysis batch : #OA-PES Laboratorium Director This result related to the samples submitted only. Suwidji Wongso, Ph.D.

5 Lampiran 1. Jumlah Hama yang Mati (ekor) pada 1 HSA (Hari Setelah Aplikasi) Perlakuan E0 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 E13 E14 E15 E16 E17 E Ulangan I II III Total Rataan Total Rataan

6 Lampiran 4 : Foto-Foto Penelitian Gambar 1: Ekstrak Minyak Gambar 2: Biji Srikaya Gambar 3: Serbuk Nimba Gambar 4: Serbuk Mahoni Gambar 5: Tween 80 Gambar 6: Wadah Penelitian

7 Gambar 7: Buah Manggis yang akan diaplikasikan Gambar 8: Aplikasi pestisida Gambar 9: Kutu putih pada buah manggis

8 Lampiran 9 77

9 PT. Angler BioChemLab LABORATORIUM PENGUJIAN MUTU MAKANAN, AIR DAN LINGKUNGAN FORMAT No. Dokumen Terbitan / revisi : 1/0 : 5.9/F/2/1 Manajer Mutu : Tanggal Terbit : Lampiran 7 :LAPORAN KONTROL KUALITAS PENGUJIAN 1 DATA SAMPEL No. 005/QA/III/12 Matrix : Manggis Measurand : Azadirachtin A, Azadirachtin B, Nimbin dan Salannin Kode Sampel Internal : No. KJU Nama Sampel dari Client No. Batch Analisa KM Manggis M Nimba 2,5 % PES12105 KM Manggis S Nimba 30 % PES12105 Tanggal Revisi : - Halaman : 1/1 2 METODE Acuan : 5.4 / IK / 2 / 1 / 1.1 Prinsip pengujian : Homogenasi sampel diekstrak menggunakan metode liquid-liquid extraction (LLE) kemudian dianalisis menggunakan LC-MS/MS. Quantitatif ditentukan berdasarkan standard addition menggunakan matrix matched pada posisi RL Definisi : 1 Standard In Solvent (SIS) : Standard dengan konsentrasi yang telah diketahui, mengandung solvent sama seperti sampel yang akan dianalisa. 2 Reporting Limit (RL) : Konsentrasi yang digunakan untuk pengujian semi quantitative, ditentukan berdasarkan pertimbangan teknis dan praktis dengan presisi dan akurasi memenuhi kriteria Quality Control 3 Matrix matched (MM) calibration Kalibrasi menggunakan matrik yang sama dengan sampel, dengan konsentrasi tertentu, ditambahkan diakhir proses preparasi dan dipreparasi seperti sampel. Alat Uji : LC-MS/MS Kriteria QC : 1 Komposisi Batch Analisa : a b c d Standard In Solvent (SIS) (awal dan akhir batch analisa) Matrix Match / MM US Sampel 2 Analisa Quality Control (QC) Presisi Repeatability mesin MM (awal dan akhir batch analisa) pada konsentrasi RL (Area MM awal - Area MM akhir)/(average (MM awal:mm akhir))*100% kriteria : RPD 20 % (< persamaan Horwitz) (1-0.5 log c) RSD 2 Konsentrasi (mg/kg) c RSD (%) E Catatan : Ok masuk kriteria X nilai tidak masuk kriteria yang ditetapkan harus ada tindakan perbaikan dan pengujian diulang

10 3 HASIL UJI No. Batch Analisa : PES12105 Kelengkapan data : MM awal MM akhir US Analisa Data : PRESISI Repeatability mesin Mesin Measurand Bracket MM % RPD QC Azadirachtin A 3.91 OK Azadirachtin B 4.61 OK Nimbin 5.06 OK Salannin 7.62 OK Hasil uji sampel No. Batch Result (mg/kg) No. Certificate Analisa Azadiractin A Azadiractin B Nimbin Salannin PES12105 ND ND ND PES12105 ND ND ND ND

11 Laporan QC No. 005/QA/III/12 73

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15 Lampiran 3 : Tabel Analisis Metode Ortogonal Kontras Pengaruh Insektisida Terhadap Mortalitas Kutu Putih Pada 2 HSA ( Hari Setelah Aplikasi) Koefisien Kontras Pestisida Tot (Perlakuan) E E E E E E E E E E E E E E E E E E E L K JKL 7381, ,185 1,852 56, ,667 24,5 28, ,75 252, ,5 420,5 108, ,