Lampiran 1. Hasil Identifikasi Tumbuhan

Transkripsi

1 Lampiran 1. Hasil Identifikasi Tumbuhan 43

2 Lampiran 2. Komite Etik Penelitian Hewan 44

3 Lampiran 3. Gambar Tumbuhan Pecut Kuda 45

4 Lampiran 4. Bagan alur penelitian uji toksisitas subkronik EEPK Hewan uji (mencit) P1: Kontrol, diberi larutan CMC Na P2: Perlakuan, diberi EEPK dosis 200 P3: Perlakuan, diberi EEPK dosis 400 mg/kg bb P4: Perlakuan, diberi EEPK dosis 800 mg/kg bb Pengamatan selama 28 hari Pengamatan setelah 28 hari Gejala toksik Berat badan Berat makanan Kematian Histopatologi Hati SGPT SGOT Makroskopik Hati Analisis data 46

5 Lampiran 5. Perhitungan volume pemberian ekstrak etanol pecut kuda dosis 200, 400, dan 800 Dosis ekstrak etanol pecut kuda yang digunakan dalam penelitian ini adalah 200, 400, dan EEPK dosis 200 Berat badan mencit = 25 g Dosis pemberian = 25 x 200 mg 1000 = 5 mg Konsentrasi ekstrak = 200 mg/10 ml Volume pemberian = 5 mg x 10 ml = 0,25 ml 200 mg 2. EEPK dosis 400 Berat badan mencit = 26,1g Dosis pemberian = 26,1 x 400 mg 1000 Konsentrasi ekstrak = 200 mg/10 ml = 10,44 mg Volume pemberian = 10,44 mg 200 mg x 10 ml = 0,52 ml 3. EEPK dosis 800 Berat badan mencit = 27,7 g Dosis pemberian = 27,7 x 800 mg 1000 Konsentrasi ekstrak = 400 mg/10 ml = 22,16 mg Volume pemberian = 22,16 mg 400 mg x 10 ml = 0,55 ml 47

6 Lampiran 6. Hasil uji pendahuluan 1. Hasil pengamatan kematian Jumlah mencit Hari ke- Persen kematian Kel K % K % K J ,5 % K4 8-1J - 1J % Keterangan: K1 = kontrol; K2 = dosis 200 ; K3 = dosis 400 ; K4 = dosis 800 ; J = Jantan 2. Hasil pengamatan gejala toksik Kelompok Gejala Toksik Hari ke- Hewan Kontrol Tremor Diare Salivasi Lemas Jalan mundur Jalan dengan perut Dosis 200 Dosis 400 Dosis 800 Tremor Diare Salivasi Lemas Jalan mundur Jalan dengan perut Tremor Diare Salivasi Lemas Jalan mundur Jalan dengan perut Tremor Diare Salivasi Lemas Jalan mundur Jalan dengan perut

7 3. Hasil rata-rata berat badan Kelompok Rata-rata berat badan (g) ± SD pada hari ke K1 27,87 ± 29,72 ± 29,06 ± 28,87 ± 4,29 29,03 ± 4,16 4,03 4,14 4,56 K2 27,61 ± 27,65 ± 27,47 ± 26,92 ± 2,63 26,78 ± 2,69 3,38 1,90 1,99 K3 27,23 ± 28,23 ± 28,44 ± 27,95 ± 6,02 27,69 ± 2,66 4,15 3,79 3,05 K4 26,09 ± 3,78 25,59 ± 3,31 24,69 ± 3,25 25,55 ± 2,57 25,35 ± 2,51 Keterangan: K1 = kontrol; K2 = dosis 200 ; K3 = dosis 400 ; K4 = dosis Hasil rata-rata berat makanan Kelompok Rata-rata konsumsi makanan (g) ± SD pada minggu ke K1 25,39 ± 0,60 24,77 ± 0,60 25,13 ± 0,87 25,05 ± 0,65 K2 24,84 ± 1,09 24,44 ± 0,94 24,71 ± 0,82 24,92 ± 0,68 K3 25,15 ± 0,92 24,91 ± 0,78 24,99 ± 0,95 25,16 ± 0,37 K4 25,10 ± 0,86 24,91 ± 1,05 25,02 ± 0,48 25,16 ± 0,91 Keterangan: K1 = kontrol; K2 = dosis 200 ; K3 = dosis 400 ; K4 = dosis

8 Lampiran 7. Hasil pengukuran kadar SGPT dan SGOT 50

9 51

10 Lampiran 8. Hasil rata-rata kadar SGPT dan SGOT 1. Hasil rata-rata pengukuran SGPT Kelompok Rata-rata kadar SGPT (IU/L) ± SD Normal 38,00 ± 5,55 Dosis 200 mg/kg BB 46,00 ± 7,21 Dosis 400 mg/kg BB 78,71 ± 4,88* Dosis 800 mg/kg BB 80,83 ± 5,77* Keterangan: * = adanya perbedaan yang signifikan (p < 0,05) 2. Hasil rata-rata pengukuran SGOT Kelompok Rata-rata kadar SGOT (IU/L) ± SD Normal 84,75 ± 14,22 Dosis 200 mg/kg BB 116,12 ± 68,02 Dosis 400 mg/kg BB 250,43 ± 36,63* Dosis 800 mg/kg BB 312,83 ± 67,24* Keterangan: * = adanya perbedaan yang signifikan (p < 0,05) 52

11 Lampiran 9. Gambar hewan percobaan Pemberian sediaan uji secara oral Pembedahan mencit 53

12 Lampiran 10. Hasil analisis spss berat badan Descriptive Statistics N Mean Deviation Minimum Maximum BBharike BBharike BBharike BBharike BBhariike One-Sample Kolmogorov-Smirnov Test BBharike1 BBharike7 BBharike14 BBharike21 BBhariike28 N Normal Parameters a Mean Deviation Most Extreme Differences Absolute Positive Negative Kolmogorov-Smirnov Z Asymp. Sig. (2-tailed) a. Test distribution is Normal. b. Calculated from data. 54

13 Lampiran 10. (Lanjutan) Descriptives 95% Confidence Interval for Mean Upper N Mean Deviation Error Lower Bound Bound Min. Max. BBharike 1 kontrol dosis dosis 400 dosis Total BBharike 7 kontrol dosis dosis 400 dosis Total BBharike 14 kontrol dosis dosis 400 dosis Total BBharike 21 kontrol dosis

14 dosis 400 dosis Total BBhariike 28 kontrol dosis dosis dosis Total

15 Lampiran 10. (Lanjutan) ANOVA Sum of Squares df Mean Square F Sig. BBharike1 Between Groups Within Groups Total BBharike7 Between Groups Within Groups Total BBharike14 Between Groups Within Groups Total BBharike21 Between Groups Within Groups Total BBhariike28 Between Groups Within Groups Total

16 Lampiran 11. Hasil analisis spss berat makanan Descriptive Statistics N Mean Deviation Minimum Maximum BM_Mingguke BM_Mingguke BM_Mingguke BM_Mingguke One-Sample Kolmogorov-Smirnov Test BM_Minggu BM_Minggu BM_Minggu BM_Minggu ke1 ke2 ke3 ke4 N Normal Parameters a Mean Deviation Most Extreme Differences Absolute Positive Negative Kolmogorov-Smirnov Z Asymp. Sig. (2-tailed) a. Test distribution is Normal. Descriptives 95% Confidence Interval for Mean Lower Upper N Mean Deviation Error Bound Bound Min. Max. BM Minggu ke1 kontrol dosis dosis dosis

17 Total BM Minggu ke2 kontrol dosis dosis dosis Total BM Minggu ke3 kontrol dosis dosis dosis Total BM Minggu ke4 kontrol dosis dosis dosis Total ANOVA Sum of Squares df Mean Square F Sig. BM_Mingguke1 Between Groups Within Groups Total BM_Mingguke2 Between Groups Within Groups Total BM_Mingguke3 Between Groups Within Groups Total BM_Mingguke4 Between Groups Within Groups Total

18 Lampiran 12. Hasil analisis spss kadar SGPT Descriptive Statistics N Mean Deviation Minimum Maximum Kadar_SGPT One-Sample Kolmogorov-Smirnov Test Kadar_SGPT N 29 Normal Parameters a Mean Deviation Most Extreme Differences Absolute.203 Positive.203 Negative Kolmogorov-Smirnov Z Asymp. Sig. (2-tailed).182 Test distribution is Normal. Kadar_SGPT Descriptives 95% Confidence Interval for Mean N Mean Deviation Error Lower Bound Upper Bound Minimum Maximum Kontrol Dosis 200 mg/kg BB Dosis 400 mg/kg BB Dosis 800 mg/kg BB Total

19 ANOVA Kadar_SGPT Sum of Squares df Mean Square F Sig. Between Groups Within Groups Total Kadar_SGPT Tukey HSD Multiple Comparisons Mean 95% Confidence Interval (I) Perlakuan (J) Perlakuan Difference (I-J) Error Sig. Lower Bound Upper Bound Kontrol Dosis 200 mg/kg BB Dosis 400 mg/kg BB * Dosis 800 mg/kg BB * Dosis 200 mg/kg BB Kontrol Dosis 400 mg/kg BB * Dosis 800 mg/kg BB * Dosis 400 mg/kg BB Kontrol * Dosis 200 mg/kg BB * Dosis 800 mg/kg BB Dosis 800 mg/kg BB Kontrol * Dosis 200 mg/kg BB * Dosis 400 mg/kg BB *. The mean difference is significant at the 0.05 level. 61

20 Lampiran 12. (Lanjutan) Homogeneous Subsets Kadar_SGPT Tukey HSD Perlakuan N Subset for alpha = Kontrol Dosis 200 mg/kg BB Dosis 400 mg/kg BB Dosis 800 mg/kg BB Sig Means for groups in homogeneous subsets are displayed. 62

21 Lampiran 13. Hasil analisis spss kadar SGOT Descriptive Statistics N Mean Deviation Minimum Maximum Kadar_SGOT One-Sample Kolmogorov-Smirnov Test Kadar_SGOT N 29 Normal Parameters a Mean Deviation Most Extreme Differences Absolute.208 Positive.208 Negative Kolmogorov-Smirnov Z Asymp. Sig. (2-tailed).161 Test distribution is Normal. Kadar_SGOT Descriptives 95% Confidence Interval for Mean N Mean Deviation Error Lower Bound Upper Bound Min. Max. Kontrol Dosis 200 mg/kg BB Dosis 400 mg/kg BB Dosis 800 mg/kg BB Total

22 Lampiran 13. (lanjutan) ANOVA Kadar_SGOT Sum of Squares df Mean Square F Sig. Between Groups Within Groups Total Multiple Comparisons Kadar_SGOT Tukey HSD 95% Confidence Interval Mean Lower Upper (I) Perlakuan (J) Perlakuan Difference (I-J) Error Sig. Bound Bound Kontrol Dosis 200 mg/kg BB Dosis 400 mg/kg BB * Dosis 800 mg/kg BB * Dosis 200 mg/kg BB Kontrol Dosis 400 mg/kg BB * Dosis 800 mg/kg BB * Dosis 400 mg/kg BB Kontrol * Dosis 200 mg/kg BB * Dosis 800 mg/kg BB Dosis 800 mg/kg BB Kontrol * Dosis 200 mg/kg BB * Dosis 400 mg/kg BB *. The mean difference is significant at the 0.05 level. 64

23 Lampiran 13. (lanjutan) Homogeneous Subsets Kadar_SGOT Tukey HSD Perlakuan N Subset for alpha = Kontrol Dosis 200 mg/kg BB Dosis 400 mg/kg BB Dosis 800 mg/kg BB Sig Means for groups in homogeneous subsets are displayed. 65

24 Lampiran 14. Hasil analisis spss kadar kreatinin Descriptive Statistics N Mean Deviation Minimum Maximum Kadar_Kreatinin One-Sample Kolmogorov-Smirnov Test Kadar_Kreatinin N 29 Normal Parameters a Mean.3159 Deviation Most Extreme Differences Absolute.181 Positive.181 Negative Kolmogorov-Smirnov Z.973 Asymp. Sig. (2-tailed).300 a. Test distribution is Normal. Kadar_Kreatinin Descriptives 95% Confidence Interval for Mean N Mean Deviation Error Lower Bound Upper Bound Minimum Max. Kontrol Dosis Dosis Dosis Total ANOVA Kadar_Kreatinin Sum of Squares df Mean Square F Sig. Between Groups Within Groups Total

25 Multiple Comparisons Kadar_Kreatinin Tukey HSD Mean 95% Confidence Interval (I) Perlakuan (J) Perlakuan Difference (I-J) Error Sig. Lower Bound Upper Bound Kontrol Dosis Dosis Dosis Dosis 200 Kontrol Dosis Dosis Dosis 400 Kontrol Dosis Dosis Dosis 800 Kontrol Dosis Dosis Kadar_Kreatinin Tukey HSD Subset for alpha = 0.05 Perlakuan N 1 Dosis Kontrol Dosis Dosis Sig..539 Means for groups in homogeneous subsets are displayed. 67

26 Lampiran 15. Hasil analisis spss kadar ureum Descriptive Statistics N Mean Deviation Minimum Maximum Kadar_Ureum One-Sample Kolmogorov-Smirnov Test Kadar_Ureum N 29 Normal Parameters a Mean Deviation Most Extreme Differences Absolute.188 Positive.188 Negative Kolmogorov-Smirnov Z Asymp. Sig. (2-tailed).254 a. Test distribution is Normal. Kadar_Ureum Descriptives 95% Confidence Interval for Mean N Mean Deviation Error Lower Bound Upper Bound Minimum Maximum Kontrol Dosis Dosis Dosis Total ANOVA Kadar_Ureum Sum of Squares df Mean Square F Sig. Between Groups Within Groups

27 ANOVA Kadar_Ureum Sum of Squares df Mean Square F Sig. Between Groups Within Groups Total Kadar_Ureum Tukey HSD Multiple Comparisons Mean 95% Confidence Interval (I) Perlakuan (J) Perlakuan Difference (I-J) Error Sig. Lower Bound Upper Bound Kontrol Dosis Dosis Dosis * Dosis 200 Kontrol Dosis Dosis Dosis 400 Kontrol Dosis Dosis Dosis 800 Kontrol * Dosis Dosis *. The mean difference is significant at the 0.05 level. Kadar_Ureum Tukey HSD Subset for alpha = 0.05 Perlakuan N 1 2 Kontrol Dosis Dosis Dosis Sig Means for groups in homogeneous subsets are displayed. 69