Lampiran 1 Identifikasi Tumbuhan

Transkripsi

1 Lampiran 1 Identifikasi Tumbuhan

2 Lampiran 2 Karakteristik Tumbuhan Temu Giring Tumbuhan Temu giring Rimpang Temu Giring Simplisia Rimpang Temu Giring

3 Lampiran 2 (sambungan) Mikroskopik serbuk simplisia temu giring pada pembesaran 10 x 40 Keterangan : 1 = Tetes Minyak Atsiri 2 = Parenkim 3 = Butir Pati 4 = Rambut penutup 5 = Fragmen Pembuluh Kayu 6 = Jaringan Gabus

4 Lampiran 3 Perhitungan Hasil Pemeriksaan Karakterisasi Simplisia dan Ekstrak 1. Perhitungan Penetapan Kadar Air a. Berat sampel = 5,094 g Volume air = 0,4 ml Kadar air = b. Berat sampel = 5,075 g Volume air = 0,5 ml Kadar air = c. Berat sampel = 5,148 g Volume air = 0,5 ml Kadar air = Kadar air rata-rata =

5 Lampiran 3 2. Perhitungan Penetapan Kadar Sari Larut dalam Air d. Berat sampel = 5,005 g Berat sari = 0,17 g Kadar sari = e. Berat sampel = 5,006 g Berat sari = 0,172 g Kadar sari = f. Berat sampel = 5,007 g Berat sari = 0,174 g Kadar sari = Kadar sari rata-rata =

6 Lampiran 3 3. Perhitungan Penetapan Kadar Sari Larut dalam Etanol g. Berat sampel = 5,088 g Berat sari = 0,12 g Kadar sari = h. Berat sampel = 5,046 g Berat sari = 0,123 g Kadar sari = i. Berat sampel = 5,059 g Berat sari = 0,113 g Kadar sari = Kadar sari rata-rata =

7 Lampiran 3 4. Perhitungan Penetapan Kadar Abu Total a. Berat sampel = 2,0116 g = 0,0920 g b. Berat sampel = 2,0069 g = 0,0910 g c. Berat sampel = 2,0104 g = 0,0909 g Kadar abu total rata-rata =

8 Lampiran 3 5. Perhitungan Penetapan Kadar Abu Tidak Larut Asam d. Berat sampel = 2,0116 g = 0,0335 g e. Berat sampel = 2,0069 g = 0,0352 g f. Berat sampel = 2,0104 g = 0,0316 g Kadar abu tidak larut asam rata-rata =

9 Lampiran 3 6. Perhitungan Penetapan Kadar Air Ekstrak j. Berat sampel = 5,077 g Volume air = 0,2 ml Kadar air = k. Berat sampel = 5,018 g Volume air = 0,2 ml Kadar air = l. Berat sampel = 5,012 g Volume air = 0,2 ml Kadar air = Kadar air rata-rata =

10 Lampiran 3 7. Perhitungan Penetapan Kadar Abu Total g. Berat sampel = 2,0003 g = 0,0080 g h. Berat sampel = 2,0004 g = 0,0081 g i. Berat sampel = 2,0004 = 0,0088 g Kadar abu total rata-rata =

11 Lampiran 3 8. Perhitungan Penetapan Kadar Abu Tidak Larut Asam a. Berat sampel = 2,0003 g = 0,0020 g b. Berat sampel = 2,0004 g = 0,0022 g c. Berat sampel = 2,0004 = 0,0020 g Kadar abu tidak larut asam rata-rata =

12 Lampiran 4 Bagan Alur Penelitian Rimpang temu giring Berat basah 9150 gram Dibersihkan dari pengotor lain Dicuci sampai bersih, ditiriskan Ditimbang Dikeringkan pada suhu ± 40 o C Ditimbang Berat kering 1037 gram Dihaluskan Serbuk simplisia 977 gram Karakterisasi Simplisia (± 100 g) Ekstraksi (877 g) Pemeriksaan makroskopik Pemeriksaan mikroskopik Penetapan kadar abu total Penetapan kadar abu tidak larut dalam asam Penetapan kadar air Penetapan kadar sari larut dalam air Penetapan kadar sari larut dalam etanol Perkolat Diuapkan menggunakan rotavapor Diperkolasi menggunakan etanol 96 % yang telah didestilasi Ampas Difreeze dryer pada suhu -40 o C Ekstrak kental 146,72 g Respon Hipersensitivitas tipe lambat Diuji efek imunomodulator Hasil + Hasil + Titer antibodi Karakterisasi ekstrak Penetapan Kadar Air

13 Lampiran 5 Alat-Alat a b a. Pipet Mikro 100 µl b. Spuit 1 ml Microtitration plate

14 Lampiran 5 Pletismometer Air Raksa Velocity 18R refrigerated centrifuge

15 Lampiran 6 Hewan Percobaan Mencit Jantan

16 Lampiran 7 Pembengkakan Kaki Mencit dan Hemaglutinasi a. b. a. Sebelum Induksi Antigen b. 24 Jam setelah Induksi Antigen

17 Lampiran 7 Aglutinasi (+) Aglutinasi (-) Hemaglutinasi Titer Antibodi

18 Lampiran 8 Hasil Perhitungan Statistik Deskripstif Descriptives Pembengkakan Titerantibodi Total Total 95% Confidence Interval for Mean N Mean Std. Deviation Std. Error Lower Bound Upper Bound Minimum Maximum Uji Normalitas Pembengkakan Titerantibodi Perlakuan *. This is a lower bound of the true significance. a. Lilliefors Significance Correction Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig * * *

19 Lampiran 9 Uji ANAVA Satu Arah (One Way ANOVA) ANOVA Pembengkakan Titerantibodi Between Groups Within Groups Total Between Groups Within Groups Total Sum of Squares df Mean Square F Sig

20 Lampiran 9 Uji Post Hoc Tuckey Multiple Comparisons Tukey HSD Dependent Variable Pembengkakan Titerantibodi (I) Perlakuan (J) Perlakuan *. The mean difference is significant at the.05 level. Me an Difference 95% Confidence Interval (I-J) Std. Error Sig. Lower Bound Upper Bound * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *

21 Homogeneous Tukey HSD a Perlak uan kontrolpos itif Sig. Lampiran 9 Pembengkakan N Subset for alpha = Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = Tukey HSD a Perlak uan kontrolpos itif Sig. Titera ntibodi N Subset for alpha = Means for groups in homogeneous subs ets are displayed. a. Uses Harmonic Mean Sample Size =