Introduction to Lab Safety

Transkripsi

1 Introduction to Lab Safety General lab rules Dr. G. Ciptadi,. Genetics and Breding/ Bioscience Lab. Dr. Marjuki : Nutrition Lab Dr. Lilik Eka R. Anim Tech. Prod. ciptadi@ub.ac.id Blog:gatotciptadi.lecturer.ub.ac.id

2 Is this what your lab looks like? Lab Safety: X Principles and practices employed to protect laboratory personnel and the environment from exposure or infection while working with living organisms, biological materials, or agents

3 Lab. Facilities and Safety

4 Back ground Lab. Function? teaching and research Important to: Rule Lab. (standart) Induction (new equipment and staff) Sharing facilities of lab. Occupational health and Safety (OHS) SOP (Standart Operational Procedure)

5 OH and S Procedure, Responsibilities Staf and Student Comply with safe working procedure: follow safe work procedure established, OH&S direction Use of appropriate personal protective equipment and safety system: where personal protective equipment, use other safety and emergency equipment provided Asssist with the preparation of risk asssesment: when requested, assist the supervisor/staff Report OH and S problems: reprort worksplace hazard to supervisor report injury or illness arising from worksplace activities

6 Weeks Pokok bahasan Sub pokok bahasan Klas F Kelas H 1. Pendahuluan/I -Kontrak kuliah, ruang lingkup MK., peraturan kerja di laboratorium, Gatot Gatot 2. Kemanan Lab -Keamanan di laboratorium. Gatot Gatot 3. Kalibrasi peralatan - Kemanana bhn kimia 4. Penanganan sampel (pakan, hasil, produk dan hasil samping peternakan, cairan rumen, darah, -Pengenalan, penanganan dan kalibrasi peralatan lab.dan bahan kimia -- Pengenalan macam dan fungsi kertas saring, milipore, dialisis, serta aplikasinya dalam suatu eksperimen Persiapan, dan pengambilan sampel. - Penanganan sampel (Chilling, Freezing, Drying) 5. produk fermentasi Produksi gas, NH3, VFA, sintesis protein mikroba 6. Instrumentasi analisis Prinsip kerja peralatan, pengertian dan cara kecernaan in vitro, in penentuan kecernaan in vitro, in sacco dan in sacco dan in vivo vivo, pakan 7. sda Prinsip kerja peralatan, pengertian dan cara penentuan kecernaan in vitro, in sacco dan in vivo. Gatot Hartutik Hartutik Hartutik Hartutik Gatot Marjuk i Marjuk i Marjuk i Marjuk i 8. UTS UTS UTS UTS

7 9 Spektrofotometri Prinsip kerja, macam dan fungsi spektrofotometri. 10 Kromatografi Prinsip kerja, macam dan fungsi kromatografi. 11 Elektroforesis Prinsip kerja, macam dan fungsi elektroforesis. 12 Haemocytometer dan ph Prinsip kerja dan fungsi meter, darah haemocytometer 13 sda Prinsip kerja, macam dan fungsi ph meter KLAS F H Imam T R Imam T Gatot Imam T Imam T R Klas Lilik Lilik R Gatot Lilik Lilik 14 Energi dan analisis serat Bomb kalorimeter, dan analisis serat Hartutik Marjuki 15 Analisis produk hasil ternak Teknik analis telur, susu, daging dan kulit Imam T Lilik R 16 UAS UAS NSF North Mississippi GK-8

8 JADWAL RENCANA PRAKTIKUM* Weeks Tanggal Materi Tempat Waktu (Koordinator) III Pengenalan alat Penimbangan, digital dan manual (lab dan lapang) IV V VI VII XIII, IX X Pengenalan ph Meter, hemositometer Pengenalan alat analisi in vitro, in sacco, in vivo, produksi gas, NH3., analisis serat., bom kalorimeter Teknik pengambilan sampel pakan, feces, urine, cairan rumen, darah UTS Pengenalan dan penjelasan ttg fungsi spektrometri Lab. NMT Lab THT Lab. NMT Lab sumber sekar Lab THT )

9 SAFETY ISSUES P E N I L A I A N : I. Individual : UTS 30 % UAS 30 % Tugas praktek 20 % I. Kelompok: Diskusi/kuis 10 % tugas terstruktur 10 %

10 Lab Safety There is always risk! The risk must be identified The risk is evaluated The risk must be measured Plan to minimize the risk Goal: Provide the highest practical protection and the lowest practical exposure

11 Safe laboratory procedures and training are needed for everyone! Important of Lab Safety: TO PREVENT: adverse health effects from exposure to chemicals/biomaterials exposure to organisms, diseases, etc. in laboratories laboratory equipment hazards - if not maintained properly

12 Lab Safety Rules 1. Always follow the teacher s directions and only do lab work when a teacher is present. 2. Conduct yourself in a responsible manner at all times. No Horseplay! 3. Do not touch any equipment, chemicals, or other materials until told to do so. 4. Do not eat food, drink beverages, or chew gum in the lab. Do not use lab glassware as food or beverage containers 5. Report ALL accidents to your assistance lecturer immediately, even if you think it is minor.

13 General lab rules For the chemicals you are working with, you should be familiar with: the standard operating procedure for using that chemical in your lab (the protocol) the hazards associated with that chemical the Protokol/procedure required for using that chemical storage requirements waste disposal procedures the procedures to be followed in the event of an emergency

14 General lab rules (cont.) Care and followed lab rules exist (Safety Issues) Avoid working alone in the lab If you must work after hours or on weekends: make arrangements with others in the building to check in with you periodically. Let someone know you are working alone, and make arrangements to call and check in periodically. avoid conducting hazardous experiments during this time.