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52 Lampiran 1. Proses pembuatan tempe KEDELAI Pembersihan Pencucian + Perendaman Perebusan (100 0 C, 30 Menit) Perendaman dengan air rebusan (22 Jam) Pengupasan kulit Perebusan dalam air asam (45 menit) Pendinginan (suhu kamar) Peragian (2 gr/kg kedelai) Pembungkusan dengan plastik Inkubasi (suhu 31 0 C, RH 78-85%; 24 jam) TEMPE
53 Lampiran 2. Proses pembuatan tepung kedelai Kedelai Penggilingan (diblender) Pengeringan (oven), suhu 45 0 C, kadar air 10% Penepungan (disk mill) Pengayakan (60 mesh) Tepung Kedelai
54 Lampiran 3. Proses pembuatan tepung tempe Tempe Mentah Pengirisan Tempe (1 x 2 x 0,5 cm 3 ) Penggilingan (diblender) Pengeringan (oven), suhu 45 0 C, kadar air 10% Penepungan (disk mill) Pengayakan (60 mesh) Tepung Tempe
55 Lampiran 4. Prosedur analisis senyawa isoflavon. Ekstraksi Tepung Kedelai dan Tepung Tempe Tepung kedelai dan tepung tempe dikeringkan selama 4 jam. Selanjutnya diekstrak dengan larutan methanol 99,8% sebanyak 2 kali, masing-masing 100 ml dengan menggunakan pompa vakum yang sudah dilapisi kertas kering, sehingga metanol yang digunakan untuk mengekstrak sebanyak 200 ml. Senyawa isoflavon termasuk senyawa polar sehingga mudah larut dengan larutan polar seperti alkohol atau metanol. Ekstraksi dilakukan 2 kali agar senyawa isoflavon dalam tepung kedelai dan tepung tempe terekstrak secara keseluruhan. Ekstrak yang diperoleh disimpan dalam ruang dingin kurang lebih 0 0 C selama satu malam untuk menggumpalkan lemaknya. Lemak yang ada dipisahkan melalui penyaringan dengan pompa vakum yang dilapisi kertas saring. Filtrat yang diperoleh selanjutnya diuapkan dengan rotavapor pada suhu 40 0 C. Ekstrak yang telah kering dilarutkan dalam 10 ml metanol absolute (p.a), kemudian disentrifuse pada kecepatan 4000 rpm selama 5 menit dengan tujuan untuk mendapatkan filtrate yang bening dan memisahkan endapan yang terbentuk. Pemurnian Isoflavon Filtrat yang terbentuk diambil 1.5 ml dan dimurnikan dalam kolom kromatografi volume 12 ml yang berisi MN-Poliamida CC6 dengan ukuran partikel 0.05-0.16 mm. Sebelum filtrate dituangkan ke dalam kolom, MN- Poliamida CC6 direndam semalam dalam kromatografi dengan larutan metanol 25%. Elusi dalam kolom dilakukan secara bertahap dengan metanol 25%, 50%, 70%, masing-masing 50 ml. Eluen yang didapat dari elusi metanol 70% (fraksi 70%) ditampung dan dikeringkan dengan rotavapor pada suhu 40 0 C sampai kering. Residu (endapan kering yang diperoleh) dilarutkan dalam 1 ml metanol absolut dan disentrifuse pada 4000 rpm selama 5 menit untuk memisahkan endapan yang ada, kemudian disaring. Filtrat yang bening siap untuk dianalisa dengan menggunakan HPLC.
56 Identifikasi Isoflavon Analisis kuantitatif dilakukan dengan kromatografi cairan tekanan tinggi (HPLC). HPLC yang digunakan berada pada kondisi sebagai berikut: Volume sampel : 40 µl Kolom : Li chrosorb RP-18 (250 x 4 mm, 5 µm) Eluen : Asam asetat 3% (pelarut A); Acetonitril (pelarut B) Gradien : 20% dalam (A + B) sampai 60% (A+B) selama 35 menit. Detektor : UV 261 nm Kecepatan alir : 0.8 ml/menit Suhu : 35 0 C
57 50 g Sampel dikeringkan pada suhu 40 0 c selama 5 jam Diektrasksi dengan metanol absolut dua kali masing-masing 100 ml, disaring dengan dengan pompa vakum Ekstrak disimpan dalam ruang pendingin 0 0 C selama semalam untuk menggumpalkan lemaknya Lemak dipisahkan dengan pompa vakum Filtrat jernih diuapkan dengan rotavapor pada suhu 40 0 C Residu dilarutkan dalam 10 ml metanol absolut Disentrifuse pada kecepatan 4000 rpm selama 5 menit Diambil 1.5 ml filtrat untuk dipurifukasi dalam kolom kromatografi yang berisi MN-Poliamida CC6 Dielusi bertahap dengan metanol 25%, 50% dan 70%, masing-masing 50 ml Hasil elusi dengan 70% metanol dikeringkan dengan rotavapor pada suhu 40 0 C Residu dilarutkan dalam ml metanol grade HPLC Disentrifusi pada 4000 rpm selama 5 menit Diambil 40 µl untuk diinjeksi pada HPLC Bagan Alir Analisis Kuantitatif Senyawa Isoflavon
58 Lampiran 5. Contoh perhitungan kandungan senyawa isoflavon. NP 289 (Tepung Kedelai). 206.37 ppm NP 290 (Tepung Tempe). 901.24 ppm
Lampiran 6. Kromatogram HPLC Standar Senyawa Isoflavon. 59
Lampiran 7. Kromatogram HPLC Tepung Kedelai. 60
Lampiran 8. Kromatogram HPLC Tepung Tempe 61
62 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU I hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu I (PPBB MI) Model 3 0.18578000 0.06192667 0.75 0.5408 Error 16 1.32980000 0.08311250 Corrected Total 19 1.51558000 R-Square Coeff Var Root MSE PPBB MI Mean 0.122580 11.86877 0.288292 2.429000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.18578000 0.06192667 0.75 0.5408 Duncan's Multiple Range Test for PPBB MI NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.083113 Number of Means 2 3 4 Critical Range.3865.4053.4171 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 2.5860 5 Non OV A A 2.4280 5 OV Tp A A 2.3640 5 Kontrol A A 2.3380 5 OV Kd
63 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU II hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu II (PPBB MII) Model 3 111.0838150 37.0279383 573.65 <.0001 Error 16 1.0327600 0.0645475 Corrected Total 19 112.1165750 R-Square Coeff Var Root MSE PPBB MII Mean 0.990789 16.74214 0.254062 1.517500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 111.0838150 37.0279383 573.65 <.0001 Duncan's Multiple Range Test for PPBB MII NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.064547 Number of Means 2 3 4 Critical Range.3406.3572.3676 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 3.5000 5 Non OV A A 3.4080 5 OV Kd B 1.4800 5 OV Tp C -2.3180 5 Kontrol
64 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU III hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu III (PPBB MIII) Model 3 144.2472150 48.0824050 478.32 <.0001 Error 16 1.6083600 0.1005225 Corrected Total 19 145.8555750 R-Square Coeff Var Root MSE PPBB MIII Mean 0.988973 13.68081 0.317053 2.317500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 144.2472150 48.0824050 478.32 <.0001 Duncan's Multiple Range Test for PPBB MIII NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.100522 Number of Means 2 3 4 Critical Range.4251.4458.4587 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 4.5720 5 Non OV B 3.4900 5 OV Tp B B 3.4780 5 Kontrol C -2.2700 5 OV Kd
65 ANALISIS STATISTIK PERSENTASE PERUBAHAN BERAT BADAN TIKUS MINGGU IV hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase perubahan berat badan minggu IV (PPBB MIV) Model 3 4.05452000 1.35150667 4.86 0.0137 Error 16 4.44916000 0.27807250 Corrected Total 19 8.50368000 R-Square Coeff Var Root MSE PPBB MIV Mean 0.476796 9.373015 0.527326 5.626000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 4.05452000 1.35150667 4.86 0.0137 Duncan's Multiple Range Test for PPBB MIV NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.278073 Number of Means 2 3 4 Critical Range.7070.7414.7629 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 6.0860 5 Non OV A A 6.0480 5 OV Tp B 5.3120 5 OV Kd B B 5.0580 5 Kontrol
66 ANALISIS STATISTIK PERSENTASE KENAIKAN BERAT BADAN TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: persentase kenaikan berat badan Model 3 6075.568100 2025.189367 10.34 0.0005 Error 16 3134.753120 195.922070 Corrected Total 19 9210.321220 R-Square Coeff Var Root MSE persentase kenaikan berat badan Mean 0.659648 43.54670 13.99722 32.14300 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 6075.568100 2025.189367 10.34 0.0005 Duncan's Multiple Range Test for persentase kenaikan berat badan NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 195.9221 Number of Means 2 3 4 Critical Range 18.77 19.68 20.25 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 56.112 5 Non OV B 35.244 5 OV Tp B B 30.110 5 Kontrol C 7.106 5 OV Kd
67 ANALISIS STATISTIK BERAT BADAN AKHIR TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 P1 P2 P3 P4 Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan akhir Model 3 2961.600000 987.200000 7.74 0.0020 Error 16 2040.400000 127.525000 Corrected Total 19 5002.000000 R-Square Coeff Var Root MSE berat badan akhir Mean 0.592083 4.277537 11.29270 264.0000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 2961.600000 987.200000 7.74 0.0020 Duncan's Multiple Range Test for berat badan akhir NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 127.525 Number of Means 2 3 4 Critical Range 15.14 15.88 16.34 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 275.600 5 P1 A A 275.600 5 P4 B 257.600 5 P3 B B 247.200 5 P2
68 ANALISIS STATISTIK BERAT UTERUS TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd Ov Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat uterus Model 3 0.35765500 0.11921833 184.83 <.0001 Error 16 0.01032000 0.00064500 Corrected Total 19 0.36797500 R-Square Coeff Var Root MSE berat uterus Mean 0.971955 7.638150 0.025397 0.332500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.35765500 0.11921833 184.83 <.0001 Duncan's Multiple Range Test for berat uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.000645 Number of Means 2 3 4 Critical Range.03405.03571.03674 Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan A 0.46600 5 Non OV A A 0.45800 5 OV Tp B 0.25000 5 OV Kd C 0.15600 5 Kontrol
69 ANALISIS STATISTIK BERAT UTERUS PER BERAT BADAN TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 Kontrol Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat uterus/berat badan tikus Model 3 0.04041500 0.01347167 145.64 <.0001 Error 16 0.00148000 0.00009250 Corrected Total 19 0.04189500 R-Square Coeff Var Root MSE berat uterus/berat badan tikus Mean 0.964674 8.048278 0.009618 0.119500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.04041500 0.01347167 145.64 <.0001 Duncan's Multiple Range Test for berat uterus/berat badan tikus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.000092 Number of Means 2 3 4 Critical Range.01289.01352.01391 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 0.166000 5 Non OV A A 0.165000 5 OV Tp B 0.092000 5 OV Kd C 0.060000 5 Kontrol
70 UJI KORELASI DAN REGRESI PERSENTASE KENAIKAN BERAT BADAN TERHADAP RASIO BERAT UTERUS PER BERAT BADAN TIKUS The SAS System The CORR Procedure 2 Variables: x y Simple Statistics Variable N Mean Std Dev Sum Minimum Maximum x 4 0.12250 0.05620 0.49000 0.06000 0.17000 y 4 32.14000 20.12323 128.56000 7.11000 56.11000 Pearson Correlation Coefficients, N = 4 Prob > r under H0: Rho=0 x y x 1.00000 0.65635 0.3437 y 0.65635 1.00000 0.3437 The GLM Procedure Number of Observations Read 4 Number of Observations Used 4 The GLM Procedure Dependent Variable: y Model 1 523.339128 523.339128 1.51 0.3437 Error 2 691.494272 345.747136 Corrected Total 3 1214.833400 R-Square Coeff Var Root MSE y Mean 0.430791 57.85400 18.59428 32.14000 Source DF Type I SS Mean Square F Value Pr > F x 1 523.3391282 523.3391282 1.51 0.3437 Source DF Type III SS Mean Square F Value Pr > F x 1 523.3391282 523.3391282 1.51 0.3437 Standard Parameter Estimate Error t Value Pr > t Intercept 3.3502375 25.1797894 0.13 0.9063 x 235.0184697 191.0247704 1.23 0.3437
71 ANALISIS STATISTIK PANJANG UTERUS KIRI TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non ov Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: panjang uterus kiri Model 3 2.35750000 0.78583333 16.81 <.0001 Error 16 0.74800000 0.04675000 Corrected Total 19 3.10550000 R-Square Coeff Var Root MSE panjang uterus kiri Mean 0.759137 8.113226 0.216217 2.665000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 2.35750000 0.78583333 16.81 <.0001 Duncan's Multiple Range Test for panjang uterus kiri NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.04675 Number of Means 2 3 4 Critical Range.2899.3040.3128 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 3.2000 5 Non OV B 2.6800 5 OV Tp B C B 2.5200 5 OV Kd C C 2.2600 5 Kontrol
72 ANALISIS STATISTIK PANJANG UTERUS KANAN TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: panjang uterus kanan Model 3 3.24950000 1.08316667 50.97 <.0001 Error 16 0.34000000 0.02125000 Corrected Total 19 3.58950000 R-Square Coeff Var Root MSE panjang uterus kanan Mean 0.905279 6.086589 0.145774 2.395000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 3.24950000 1.08316667 50.97 <.0001 Duncan's Multiple Range Test for panjang uterus kanan NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.02125 Number of Means 2 3 4 Critical Range.1954.2050.2109 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 3.08000 5 Non OV B 2.28000 5 OV Tp B C B 2.16000 5 OV Kd C C 2.06000 5 Kontrol
73 ANALISIS STATISTIK TOTAL PANJANG UTERUS TIKUS hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: total panjang uterus Model 3 10.95401500 3.65133833 2938.70 <.0001 Error 16 0.01988000 0.00124250 Corrected Total 19 10.97389500 R-Square Coeff Var Root MSE totalpanjanguterus Mean 0.998188 0.696692 0.035249 5.059500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 10.95401500 3.65133833 2938.70 <.0001 Duncan's Multiple Range Test for total panjang uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.001243 Number of Means 2 3 4 Critical Range.04726.04956.05100 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 6.28000 5 Non Ov B 4.95800 5 OV Tp C 4.68000 5 OV Kd D 4.32000 5 Kontrol
74 ANALISIS STATISTIK BERAT BASAH BEBAS LEMAK UTERUS TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat basah bebas lemak uterus Model 3 0.07625500 0.02541833 78.21 <.0001 Error 16 0.00520000 0.00032500 Corrected Total 19 0.08145500 R-Square Coeff Var Root MSE berat basah bebas lemak uterus Mean 0.936161 9.081993 0.018028 0.198500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.07625500 0.02541833 78.21 <.0001 Duncan's Multiple Range Test for berat basah bebas lemak uterus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.000325 Number of Means 2 3 4 Critical Range.02417.02535.02608 Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan A 0.26200 5 Non Ov A A 0.25400 5 OV Tp B 0.16200 5 OV Kd C 0.11600 5 Kontrol
75 ANALISIS STATISTIL BERAT KERING BEBAS LEMAK UTERUS TIKUS Hasil Penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat kering bebas lemak uterus Model 3 0.00453500 0.00151167 75.58 <.0001 Error 16 0.00032000 0.00002000 Corrected Total 19 0.00485500 R-Square Coeff Var Root MSE berat kering bebas lemaku terus Mean 0.934089 12.25243 0.004472 0.036500 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 0.00453500 0.00151167 75.58 <.0001 Duncan's Multiple Range Test for berat kering bebas lemaku terus NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 0.00002 Number of Means 2 3 4 Critical Range.005996.006288.006470 Means with the same letter are not significantly different. Duncan Grouping Mean N Perlakuan A 0.058000 5 Non OV B 0.042000 5 OV Tp C 0.028000 5 OV Kd D 0.018000 5 Kontrol
76 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU I hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu I Model 3 13689.45000 4563.15000 11.37 0.0003 Error 16 6419.51200 401.21950 Corrected Total 19 20108.96200 R-Square Coeff Var Root MSE berat badan minggu I Mean 0.680764 9.530601 20.03046 210.1700 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 13689.45000 4563.15000 11.37 0.0003 Duncan's Multiple Range Test for berat badan minggu I NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 401.2195 Number of Means 2 3 4 Critical Range 26.86 28.16 28.98 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 241.34 5 OV Kd A A 229.20 5 OV Tp B 193.56 5 Kontrol B B 176.58 5 Non OV
77 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU II hasil penelitian Class Level Information Class Levels Values perlakuan 4 Nn OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu II Model 3 13108.70000 4369.56667 12.06 0.0002 Error 16 5796.99200 362.31200 Corrected Total 19 18905.69200 R-Square Coeff Var Root MSE berat badan minggu II Mean 0.693373 8.780559 19.03450 216.7800 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 13108.70000 4369.56667 12.06 0.0002 Duncan's Multiple Range Test for berat badan minggu II NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 362.312 Number of Means 2 3 4 Critical Range 25.52 26.76 27.54 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 257.80 5 OV Kd B 214.94 5 Non OV B C B 206.36 5 OV Tp C C 188.02 5 Kontrol
78 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU III hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu III Model 3 4913.91350 1637.97117 4.45 0.0186 Error 16 5887.16400 367.94775 Corrected Total 19 10801.07750 R-Square Coeff Var Root MSE berat badan minggu III Mean 0.454947 8.330025 19.18196 230.2750 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 4913.913500 1637.971167 4.45 0.0186 Duncan's Multiple Range Test for berat badan minggu III NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 367.9477 Number of Means 2 3 4 Critical Range 25.72 26.97 27.75 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 245.96 5 OV Tp A A 241.10 5 Non OV A B A 228.60 5 OV Kd B B 205.44 5 OV Tp
79 ANALISIS STATISTIK BERAT BADAN TIKUS MINGGU III hasil penelitian Class Level Information Class Levels Values perlakuan 4 Non OV Kontrol OV Kd OV Tp Number of Observations Read 20 Number of Observations Used 20 Dependent Variable: berat badan minggu IV Model 3 2961.600000 987.200000 7.74 0.0020 Error 16 2040.400000 127.525000 Corrected Total 19 5002.000000 R-Square Coeff Var Root MSE berat badan minggu IV Mean 0.592083 4.277537 11.29270 264.0000 Source DF Anova SS Mean Square F Value Pr > F perlakuan 3 2961.600000 987.200000 7.74 0.0020 Duncan's Multiple Range Test for berat badan minggu IV NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 16 Error Mean Square 127.525 Number of Means 2 3 4 Critical Range 15.14 15.88 16.34 Means with the same letter are not significantly different. Duncan Grouping Mean N perlakuan A 275.600 5 Non OV A A 275.600 5 OV Tp B 257.600 5 OV Kd B B 247.200 5 Kontrol
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