LAMPIRAN. Lampiran 1. Tabel cara kerja Pengukuran Aktivitas Protease Digesti Kasein 5% Buffer

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1 LAMPIRA Lampiran 1. Tabel cara kerja Pengukuran Aktivitas Protease Digesti VOLUME (Ml) PEREAKSI Tirosin Standard Sampel Kontrol Balanko standard Kasein 5% Buffer Ekstrak enzim Akuades TCA Di Inkubasi selama 60 menit pada suhu 37 0 c TCA Kasein 1% Di masukkan semua tabung kedalama refrigerator selama minimal 1 jam Sentrifugasi pada kecepatan 6000 rpm selama 15 menit Ukur absorbansinya pada panjang gelombang 280 nm

2 Lampiran 2. Tabel Cara Kerja Pengukuran Aktivitas Amilase Digesti VOLUME (Ml) PEREAKSI Maltosa Standard Sampel Kontrol Balanko standard Amilum 1% Ekstrak enzim Akuades DS Inkubasi selama 30 menit pada suhu 37 0 c DS Amilum 1% Di masukkan semua tabung kedalama refrigerator selama 5 menit Setelah dingin di tambahkan akuades sebanyak 1.5 ml Ukur absorbansinya pada panjang gelombang 540 nm

3 Lampiran 3. Tabel cara kerja Penentuan Kadar Protein Supernatan VOLUME (Ml) PEREAKSI Kasein Standard Sampel Kontrol Balanko standard Ekstrak enzim Pereaksi C Inkubasi selama 30 menit pada suhu 37 0 c Pereaksi D Di kocok segera dan biarkan pada suhu kamar selama paling sedikit 10 menit Ukur absorbansinyapada panjang gelombang 660 nm Kadar protein sampel di tentukan dengan kurva standard

4 Lampiran 4. Analisis Aktivitas Protease pada epatopankreas dan Intestine Univariate Analysis of Variance Descriptive Statistics B S K Mean Std. Deviation Tests of Between-Subjects Effects Source Corrected Model Intercept * Error Corrected Type III Sum of Squares df Mean Square F Sig..372 a a. R Squared =.465 (Adjusted R Squared =.316) Post oc Tests Estimates 95% Confidence Interval Mean Std. Error Lower Bound Upper Bound

5 Pairwise Comparisons (I) (J) Mean Difference Based on estimated marginal means *. The mean difference is significant at the.05 level. 95% Confidence Interval for Difference a (I-J) Std. Error Sig. a Lower Bound Upper Bound -.200* * a. Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments). Univariate Tests Contrast Error Sum of Squares df Mean Square F Sig The F tests the effect of. This test is based on the linearly independent pairwise comparisons among the estimated marginal means. 3. * B S K 95% Confidence Interval Mean Std. Error Lower Bound Upper Bound

6 Lampiran 5. asil Analisis Aktivitas Amilase pada epatopankreas dan Intestine Univariate Analysis of Variance Between-Subjects Factors Value Label B 8 S 8 K Descriptive Statistics B S K Mean Std. Deviation Tests of Between-Subjects Effects Source Corrected Model Intercept * Error Corrected Type III Sum of Squares df Mean Square F Sig a a. R Squared =.739 (Adjusted R Squared =.666)

7 Post oc Tests Estimates 95% Confidence Interval Mean Std. Error Lower Bound Upper Bound Pairwise Comparisons (I) (J) Mean Difference Based on estimated marginal means *. The mean difference is significant at the.05 level. 95% Confidence Interval for Difference a (I-J) Std. Error Sig. a Lower Bound Upper Bound -.556* * a. Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments). Univariate Tests Contrast Error Sum of Squares df Mean Square F Sig The F tests the effect of. This test is based on the linearly independent pairwise comparisons among the estimated marginal means. 3. * B S K 95% Confidence Interval Mean Std. Error Lower Bound Upper Bound

8 Lampiran 6. Kurva Standar Larutan Tirosin Absorbansi 0,6 0,5 0,4 0,3 0,2 0,1 0 Y = a + bx 0 0,05 0,1 0,25 0,5 1,0 Konsentrasi (mg/ml)

9 Lampiran 7. Kurva Standar Larutan Maltosa 540 nm 0,01 0,05 0,10 0,25 mg/ml

10 Lampiran Gambar Penelitian Gambar 1. Akuarium ikan uji Gambar 2. Timbangan Mekanik Gambar 3. Saluran digesti ikan nilem Gambar 4. Running Enzim Gambar 5. Water eater Gambar 6. Sentrifugase

11 Gambar 7. Oven Gambar 8. Timbangan Analitik Gambar 9. omogenizer Gambar 10. Spectrophotometer tipe U-3900