62 Lampiran 1.a Data Kadar Air Kelopak Rosella Kadar air (%) = kehilangan berat (g) x 100 Sampel sebelum kering (g) Kehilangan berat = berat sampel mula-mula berat sampel setelah dikeringkan Kadar air (%) = 2-0,38 x 100 2 = 81% Lampiran 1.b Data nilai ph ekstrak ph awal Kondisi Ekstraksi Ulangan ph 70 O C 15 menit 1 2,85 2 2,85 70 O C 30 menit 1 2,85 2 2,85 85 O C 15 menit 1 2,87 2 2,94 85 O C 30 menit 1 2,80 2 2,90 100 O C 15 menit 1 2,79 2 2,77 100 O C 30 menit 1 2,78 2 2,94
63 Lampiran 2. Uji Anova nilai ph Univariate Analysis of Variance Dependent Variable:pH Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Corrected Model.016 a 5.003.941.516 Intercept 97.413 1 97.413 2.858E4.000 Waktu.000 1.000.061.813 Suhu.007 2.003.971.431 waktu * suhu.009 2.005 1.352.328 Error.020 6.003 Total 97.449 12 Corrected Total.036 11 a. R Squared =,440 (Adjusted R Squared = -,027)
64 Lampiran 3. Data total fenol Kurva standar Absorbansi Kurva Standar Larutan Asam Galat y = 0,0044x - 0,1021 R² = 0,9902 Konsentrasi (ppm) Kondisi Ekstraksi Ulangan Absorbansi Konsentrasi (ppm) Konsentrasi ( mg GAE/g rosella basah ) Rata-rata 70 O C 15 menit 1 0,364 105,93 0,49 0,49 2 0,364 105,93 0,49 70 O C 30 menit 1 0,404 115,02 0,53 0,53 2 0,405 115,25 0,53 85 O C 15 menit 1 0,426 120,02 0,55 0,57 2 0,452 125,93 0,58 85 O C 30 menit 1 0,404 112,75 0,52 0,55 2 0,459 127,52 0,59 100 O C 15 menit 1 0,491 134,80 0,62 0,59 2 0,436 122,30 0,56 100 O C 30 menit 1 0,544 146,84 0,68 0,67 2 0,543 146,61 0,67 Contoh perhitungan total fenol X : konsentrasi asam galat; y : absorbansi Y = 0,0044x- 0,1021 0,364= 0,0044x 0,1021 X= 105,93 ppm Konsentrasi (mg GAE/g) = 105,93 mg asam galat x 230 ml 1000 ml ekstrak 50g = 0,49 mg GAE/g
65 Lampiran 4. Uji Anova total fenol Univariate Analysis of Variance Dependent Variable:fenol Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Corrected Model.039 a 5.008 9.922.007 Intercept 3.865 1 3.865 4.882E3.000 Waktu.004 1.004 5.568.056 Suhu.030 2.015 19.168.002 waktu * suhu.005 2.002 2.853.135 Error.005 6.001 Total 3.909 12 Corrected Total.044 11 a. R Squared =,892 (Adjusted R Squared =,802) Post Hoc Tests Suhu Homogeneous Subsets Fenol Duncan Subset suhu N 1 2 3 70 4.5100 85 4.5600 100 4.6325 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) =,001.
66 ANOVA Fenol Sum of Squares df Mean Square F Sig. Between Groups.039 5.008 9.922.007 Within Groups.005 6.001 Total.044 11 Post Hoc Tests Interaksi Homogeneous Subsets Duncan Fenol interaks i N Subset for alpha = 0.05 1 2 3 1 2.4900 2 2.5300.5300 4 2.5550.5550 3 2.5650 5 2.5900 6 2.6750 Sig..067.089 1.000 Means for groups in homogeneous subsets are displayed.
67 Lampiran 5. Data total asam tertitrasi Kondisi ekstraksi sampel Ulangan Berat sampel (g) ml 0,1 NaOH TAT (%) Rata-rata ph 70 O C 15 menit 1 10,078 0,8 6,569 6,63 2,85 2 10,107 0,8 6,699 2,85 70 O C 30 menit 1 10,098 0,7 5,404 5,43 2,85 2 10,109 0,7 5,449 2,85 85 O C 15 menit 1 10,1 0,7 5,642 5,74 2,84 2 10,008 0,7 5,839 2,87 85 O C 30 menit 1 10,026 0,7 5,600 5,57 2,8 2 10,102 0,7 5,532 2,9 100 O C 15 menit 1 10,009 0,8 6,458 6,23 2,77 2 10,099 0,8 6,007 2,79 100 O C 30 menit 1 10,034 1,1 9,130 9,06 2,94 2 10,106 1,1 8,986 2,78 Contoh perhitungan total asam tertitrasi: Total asam tertitrasi = ml 0,1 NaOH x N NaOH x Faktor Pengenceran x 100% gram ekstrak 6,569% = 0,8 x 0,08 x 10 x 100% 10,078
68 Lampiran 6. Uji Anova total asam tertitrasi Univariate Analysis of Variance Dependent Variable:totalasam Tests of Between-Subjects Effects Source Type III Sum of Squares Df Mean Square F Sig. Corrected Model 18.460 a 5 3.692 154.697.000 Intercept 498.199 1 498.199 2.087E4.000 Waktu.691 1.691 28.961.002 Suhu 8.985 2 4.493 188.236.000 waktu * suhu 8.784 2 4.392 184.025.000 Error.143 6.024 Total 516.802 12 Corrected Total 18.604 11 a. R Squared =,992 (Adjusted R Squared =,986) Duncan Post Hoc Tests suhu Homogeneous Subsets Subset Suhu N 1 2 3 85 4 5.6525 70 4 6.0300 100 4 7.6475 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) =,024.
69 Waktu Dependent Variable:total asam tertitrasi waktu Mean Std. Error Lower Bound 95% Confidence Interval Upper Bound 15 6.203.063 6.049 6.358 30 6.683.063 6.529 6.838 ANOVA Totalasam Sum of Squares df Mean Square F Sig. Between Groups 18.460 5 3.692 154.697.000 Within Groups.143 6.024 Total 18.604 11 Duncan Post Hoc Tests Interaksi Homogeneous Subsets Subset kombinasi N 1 2 3 4 70*30 2 5.4250 85*30 2 5.5650 85*15 2 5.7400 100*15 2 6.2350 70*15 2 6.6350 100*30 2 9.0600 Sig..096 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) =,024.
70 Lampiran 7.a. Data inhibisi ekstrak rosella ph awal terhadap enzim α-amilase Bacillus sp Kondisi Ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15mnt 1 95,67 94,72 93,78 2 93,23 92,90 92,56 70 0 C, 30mnt 1 95,13 94,32 93,51 2 93,91 91,54 89,18 85 0 C, 15mnt 1 98,65 97,63 96,62 2 94,59 96,96 99,32 85 0 C, 30mnt 1 93,23 88,50 83,76 2 99,32 99,66 100,00 100 0 C, 15mnt 1 92,56 95,60 98,65 2 93,23 91,20 89,18 100 0 C, 30mnt 1 99,32 99,66 100,00 2 95,26 97,63 100,00
71 Lampiran 7.b. Data inhibisi ekstrak rosella ph saluran pencernaan terhadap enzim α-amilase Bacillus sp Kondisi ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15mnt 1 37,28 36,95 36,63 2 42,29 43,83 45,37 70 0 C, 30mnt 1 8,74 8,29 7,84 2 8,74 8,68 8,61 85 0 C, 15mnt 1 33,42 27,83 22,24 2 12,98 10,15 7,33 85 0 C, 30mnt 1 7,84 8,61 9,38 2 9,25 9,90 10,54 100 0 C, 15mnt 1 13,88 16,00 18,12 2 11,70 11,83 11,95 100 0 C, 30mnt 1 12,98 12,08 11,18 2 12,47 11,05 9,64
72 Lampiran 7. c. Data inhibisi Acarbosa terhadap kerja enzim α-amilase Bacillus sp - ph awal Sampel Inhibisi (%) Rata-rata (%) Acarbosa 98,76 98,76 98,76 - ph simulasi sistem pencernaan Sampel Inhibisi (%) Rata-rata (%) Acarbosa 65,21 65,21 65,21
73 Lampiran 8. Uji Anova daya inhibisi ekstrak rosella terhadap enzim α-amilase Bacillus sp a. ph awal Univariate Analysis of Variance Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 70.461 a 6 11.744 1.030.477 Intercept 117401.149 1 117401.149 1.030E4.000 Waktu.442 1.442.039.850 Suhu 16.711 2 8.356.733.514 waktu * suhu 38.158 2 19.079 1.674.255 Error 79.795 7 11.399 Total 127705.054 14 Corrected Total 150.256 13 a. R Squared =.469 (Adjusted R Squared =.014)
74 b. ph saluran pencernaan Univariate Analysis of Variance Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 5363.791 a 6 893.965 33.466.000 Intercept 10799.861 1 10799.861 404.298.000 Waktu 638.502 1 638.502 23.903.002 Suhu 320.246 2 160.123 5.994.030 waktu * suhu 465.677 2 232.838 8.716.013 Error 186.988 7 26.713 Total 13555.130 14 Corrected Total 5550.780 13 a. R Squared =.966 (Adjusted R Squared =.937) Duncan Post Hoc Tests suhu Homogeneous Subsets suhu N Subset 1 2 3 100 4 12.7410 85 4 14.1228 70 4 24.3250 control 2 65.0000 Sig..745 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 26.713.
75 Waktu Dependent Variable:amilase6.8 95% Confidence Interval waktu Mean Std. Error Lower Bound Upper Bound 15 24.357 2.279 18.781 29.934 30 9.768 2.279 4.192 15.345 ANOVA amilase6.8 Sum of Squares Df Mean Square F Sig. Between Groups 5363.791 6 893.965 33.466.000 Within Groups 186.988 7 26.713 Total 5550.780 13 Duncan Post Hoc Tests Interaksi Homogeneous Subsets Subset for alpha = 0.05 Interaksi N 1 2 3 70*30 2 8.4830 85*30 2 9.2545 100*30 2 11.5680 100*15 2 13.9140 85*15 2 18.9910 70*15 2 40.1670 Kontrol 2 65.0000 Sig..099 1.000 1.000 Means for groups in homogeneous subsets are displayed.
76 Lampiran 9. Data inhibisi ekstrak rosella ph awal terhadap enzim α-amilase pankreas babi ph awal Kondisi Ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15menit 1 95,43 96,15 96,88 2 82,45 84,14 85,82
77 Lampiran 10.a. Data inhibisi ekstrak rosella ph awal terhadap enzim α-glukosidase Kondisi Ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15mnt 1 91,77 90,59 89,41 2 95,29 96,47 97,65 70 0 C, 30mnt 1 97,65 92,94 88,24 2 89,41 91,77 94,12 85 0 C, 15mnt 1 88,24 88,24 88,24 2 88,24 87,65 87,06 85 0 C, 30mnt 1 84,70 85,88 87,06 2 89,41 90,59 91,77 100 0 C, 15mnt 1 89,41 88,82 88,24 2 78,82 76,47 74,12 100 0 C, 30mnt 1 88,24 84,12 80,00 2 94,118 94,706 95,294
78 Lampiran 10.b. Data inhibisi ekstrak rosella ph pencernaan terhadap enzim α-glukosidase Kondisi ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15mnt 1 37,28 36,95 36,63 2 42,29 43,83 45,37 70 0 C, 30mnt 1 8,74 8,29 7,84 2 8,74 8,68 8,61 85 0 C, 15mnt 1 33,42 27,83 22,24 2 12,98 10,15 7,33 85 0 C, 30mnt 1 7,84 8,61 9,38 2 9,25 9,90 10,54 100 0 C, 15mnt 1 13,88 16,00 18,12 2 11,70 11,83 11,95 100 0 C, 30mnt 1 12,98 12,08 11,18 2 12,47 11,05 9,64
79 Lampiran 10.c. Data inhibisi acarbosa terhadap enzim α-glukosidase - ph awal Sampel Inhibisi (%) Rata-rata (%) Acarbosa 100,00 100,00 100,00 - ph saluran pencernaan Sampel Inhibisi (%) Rata-rata (%) Acarbosa 100,00 100,00 100,00
80 Lampiran 11. Uji Anova daya inhibisi ekstrak rosella terhadap enzim α- glukosidase a. ph awal Univariate Analysis of Variance Dependent Variable:gluksidase Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 354.662 a 6 59.110 2.561.122 Intercept 107785.848 1 107785.848 4.669E3.000 Waktu 11.533 1 11.533.500.503 Suhu 100.746 2 50.373 2.182.183 waktu * suhu 35.696 2 17.848.773.497 Error 161.594 7 23.085 Total 115403.581 14 Corrected Total 516.256 13 a. R Squared =.687 (Adjusted R Squared =.419)
81 Lampiran 12.a. Data inhibisi ekstrak rosella ph awal terhadap kerja enzim lipase Kondisi Ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15mnt 1 89,66 88,95 88,24 2 91,31 90,21 89,11 70 0 C, 30mnt 1 88,96 89,59 90,21 2 94,71 94,76 94,81 85 0 C, 15mnt 1 89,16 89,39 89,61 2 96,33 95,73 95,13 85 0 C, 30mnt 1 97,43 97,03 96,63 2 90,73 91,35 91,96 100 0 C, 15mnt 1 85,51 86,14 86,76 2 85,44 85,95 86,46 100 0 C, 30mnt 1 85,32 85,61 85,91 2 85,81 85,95 86,09
82 Lampiran 12.b. Data inhibisi ekstrak rosella ph pencernaan terhadap kerja enzim lipase Kondisi Ekstraksi Ulangan Inhibisi (%) Rata-rata (%) 70 0 C, 15menit 1 6,54 6,35 6,15 2 6,44 6,78 7,12 70 0 C, 30menit 1 5,00 6,06 7,12 2 7,40 6,01 4,62 85 0 C, 15menit 1 0,00 0,00 0,00 2 7,12 5,29 3,46 85 0 C, 30menit 1 4,52 5,43 6,35 2 2,50 4,09 5,67 100 0 C, 15menit 1 50,58 49,33 48,078 2 32,98 32,98 32,98 100 0 C, 30menit 1 31,73 36,11 40,48 2 31,92 31,59 31,25
83 Lampiran 13. Uji Anova daya inhibisi ekstrak rosella terhadap enzim lipase a. ph awal Univariate Analysis of Variance Tests of Between-Subjects Effects Dependent Variable:lipase Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 124.723 a 5 24.945 2.964.109 Intercept 97316.135 1 97316.135 1.156E4.000 Waktu 5.221 1 5.221.620.461 Suhu 115.288 2 57.644 6.850.028 waktu * suhu 4.214 2 2.107.250.786 Error 50.494 6 8.416 Total 97491.351 12 Corrected Total 175.216 11 a. R Squared =.712 (Adjusted R Squared =.472) Post Hoc Tests suhu Homogeneous Subsets Lipase Duncan Subset suhu N 1 2 100 4 85.9142 70 4 90.8748 90.8748 85 4 93.3722 Sig..052.269 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8.416.
84 Tests of Between-Subjects Effects Dependent Variable:lipase Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 124.723 a 5 24.945 2.964.109 Intercept 97316.135 1 97316.135 1.156E4.000 interaksi 124.723 5 24.945 2.964.109 Error 50.494 6 8.416 Total 97491.351 12 Corrected Total 175.216 11 a. R Squared =.712 (Adjusted R Squared =.472) Post Hoc Tests interaksi Homogeneous Subsets Duncan Subset interaksi N 1 2 100C30 2 85.7830 100C15 2 86.0455 70C15 2 89.5795 89.5795 70C30 2 92.1700 92.1700 85C15 2 92.5575 92.5575 85C30 2 94.1870 Sig..070.180 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8.416.
85 b. ph saluran pencernaan Univariate Analysis of Variance Tests of Between-Subjects Effects Dependent Variable:lipase6.8 Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 3036.151 a 5 607.230 25.230.001 Intercept 2840.502 1 2840.502 118.023.000 Waktu 3.247 1 3.247.135.726 Suhu 2959.155 2 1479.577 61.476.000 waktu * suhu 73.749 2 36.875 1.532.290 Error 144.405 6 24.067 Total 6021.057 12 Corrected Total 3180.555 11 a. R Squared =.955 (Adjusted R Squared =.917) Post Hoc Tests suhu Homogeneous Subsets Duncan lipase6.8 Subset Suhu N 1 2 85 4 2.3800 70 4 6.2982 100 4 37.4778 Sig..302 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 24.067.
86 Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 3036.151 a 5 607.230 25.230.001 Intercept 2840.502 1 2840.502 118.023.000 interaksi 3036.151 5 607.230 25.230.001 Error 144.405 6 24.067 Total 6021.057 12 Corrected Total 3180.555 11 a. R Squared =.955 (Adjusted R Squared =.917) Post Hoc Tests interaksi Homogeneous Subsets Duncan Subset interaksi N 1 2 85C15 2.0000 85C30 2 4.7600 70C30 2 6.0340 70C15 2 6.5625 100C30 2 33.8015 100C15 2 41.1540 Sig..248.185 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 24.067.
87 Lampiran 14. Penentuan konsentrasi ekstrak [Ekstrak (M)] Ulangan Inhibisi (%) Rata-rata (%) 0,25 1 0,97 2 0,97 0,97 0,50 1 0,97 2 0,98 0,98 0,75 1 0,97 2 0,95 0,96 1 1 0,97 2 0,97 0,97
88 Lampiran 15. Kinetika inhibisi ekstrak rosela 1. Standar Maltosa [maltosa] (%) Absorbansi 0,00 0,235 0,20 0,406 0,40 0,557 0,60 0,699 0,80 0,868 1,00 0,998 y = 0,7633x + 0,2455 R² = 0,9988 Absorbansi Konsentrasi maltosa (%) Kurva standar maltosa
89 1. Standar (pati+ enzim) [pati] Absorbansi Lampiran 15 lanjutan [pati] bereaksi rataan Kecepatan rataan 1/[pati] 1/kecepatan rataan 1,000 0,8 0,73 0,07 1,000 13,77 1,250 0,98 0,96 0,09 0,800 10,39 1,500 1,145 1,18 0,12 0,667 8,49 1,750 1,269 1,34 0,13 0,571 7,46 2,000 1,456 1,59 0,16 0,500 6,31 2,250 1,646 1,84 0,18 0,444 5,45 2,500 1,853 2,11 0,21 0,400 4,75 2. Sampel (pati+enzim+ekstrak rosela) [pati] Absorbansi [pati] bereaksi rataan Kecepatan rataan 1/[pati] 1/Kecepatan rataan 1,000 0,369 0,162 0,02 1,000 61,81 1,250 0,436 0,250 0,03 0,800 40,07 1,500 0,459 0,280 0,03 0,667 35,75 1,750 0,557 0,408 0,04 0,571 24,50 2,000 0,593 0,455 0,05 0,500 21,97 2,250 0,674 0,561 0,06 0,444 17,81 2,500 0,719 0,620 0,06 0,400 16,12
90 Lampiran 15. Lanjutan 1.Plot Lineweaver-Burk y = 56,013x - 2,7449 R² = 0,9541 non inhibitor inhibitor 1/V y = 13,358x - 0,1936 R² = 0,9959 1/ [S] Contoh perhitungan: Plot Lineweaver-Burk inhibitor: 1/V maks = 15,65mM V maks = 1/ 15,65mM = 0,06 Km/ V maks Km/ 0,06 Km = 74,78mM = 74,78mM = 4,77mM