Lampiran 1. Komposisi media RPMI-1640 Komponen Konsentrasi (mg/l) Garam anorganik Ca(NO ).4H O 100 KCl 400 MgSO 4.7H O 100 NaCl 6000 NaHCO 000 Na HPO 4 anhydrous 800 Komponen lain D-Glukosa 000 fenol merah 5, glutathione (tereduksi) 1 HEPES (d) 5958 Asam Amino L-arginin HCl 00 L-asparagin 56,8 L-asam aspartat 0 L-sistin 50 L-asam glutamat 0 L-glutamin 00 Glisin 10 L-histidin 15 L-hidroksiprolin 0 L-isoleusin 50 L-leusin 50 L-lisin HCl 40 L-metionin 15 L-fenilalanin 15 L-prolin 0 L-serin 0 L-threonin 0 L-triptofan 5 L-tirosin 0 L-valin 0 Vitamin D-biotin 0, B-Ca pantotenat 0,5 kolin klorida asam folat 1 i-inositol 5 11
Lanjutan tabel 1 Komponen Konsentrasi (mg/l) nikotinamida 1 PABA 1 Piridoksal HCl 1 Riboflavin 0, Tiamin HCl 1 Vitamin B1 0,005 114
Lampiran. Komposisi balanced salt solution PBS (Harrison and Rae, 1997) Komponen Konsentrasi (mg/l) CaCl.H O 1,5 KCl 00 KH PO 4 00 MgCl.6H O 100 NaCl 8000 Na HPO 4 1150 115
Lampiran. Proses Produksi Pangan Iradiasi yang shelf-stable (IAEA, 1995) Langkah kunci dalam produksi pangan iradiasi adalah sebagai berikut: 1. Masaklah terlebih dahulu daging mentah, unggas, dan seafood sampai keadaan matang (medium done) dengan syarat suhu internalnya kira-kira 75 C, untuk menginaktivasi enzim pengurai jaringan, kecuali bahan yang dimasak telah diproses secara termal sebelumnya (misalnya diasapkan (hot-smoked), dikuring, atau dibuat pastrami). Secara hermetis kemaslah secara vakum dalam kemasan individu yang berupa kantong multilapis alumunium foil, seperti yang dikembangkan oleh program US Army dan disetujui oleh FDA atau yang setara.. Bekukan produk dalam kemasan secara kriogenik atau mekanis hingga mencapai -40 C dan iradiasi saat masih dalam keadaan beku untuk mempertahankan kualitas gizi. Menumpuk kemasan dengan dry ice merupakan cara yang efektif dan tidak mahal untuk mempertahankan keadaan beku selama proses iradiasi yang panjang. 4. Untuk iradiasi gamma atau sinar X, kemas kantong-kantong pengemas individual beku dalam karton pengemas dan simpan dalam keadaan beku atau dengan dry ice sebelum diiradiasi. Jika waktu iradiasi lebih dari satu jam, dry ice harus ditaruh di sekitar karton pengemas untuk mempertahankan keadaan beku (hard frozen) selama proses iradiasi. Bahkan pada energy maksimum 10 MeV iradiasi electron /electron beam (yang memiliki daya penetrasi rendah) harus diterapkan pada tiap kantong produk yang dibekukan, misalnya: kantong yang diletakkan dalam posisi tidur pada ban berjalan yang membawa produk-produk tersebut saat diiradiasi dengan electron beam. Kantong yang berukuran lebih tebal daripada beberapa sentimeter harus diiradiasi pada tiap sisi (two-pass, 50% dari total minimum dosis pada tiap kali) untuk mencapai keseragaman. Laju dosis iradiasi dengan electron beam normalnya merupakan dosis yang sangat tinggi sehingga tidak membutuhkan penanganan aktif untuk mempertahankan keadaan beku selama iradiasi (waktu paparan normalnya 116
terlalu sebentar untuk kemungkinan terjadinya kenaikan suhu yang signifikan). 5. Lakukan proses iradiasi dengan dosis minimum yang telah ditentukan (5-45 kgy tergantung produknya) untuk memberikan tingkat 10-1 jaminan sterilitas antibotulinum dengan menggunakan dosimetri yang sesuai. Dosis minimum yang dibutuhkan untuk berbagai produk telah ditentukan oleh studi produk yang diinokulasi dengan C. botulinum yang dilakukan oleh program US Army. Pada level dosis minimum ini, produk steril secara mikrobiologi dan dapat disimpan selama bertahun-tahun dalam kondisi suhu ruang karena enzim endogen yang akan mendegradasi produk, meskipun tidak ada mikroorganisme yang hidup, telah diinaktivasi dengan panas sebelum dikemas dan diiradiasi. 6. Segera setelah iradiasi, biarkan suhu produk naik secara perlahan hingga mencapai suhu ruang. Kemas secara individu kantong-kantong produk dalam karton pengemas seperti saat sebelum diiradiasi. 117
Lampiran 4. Tabel data hasil pembacaan absorbansi uji proliferasi limfosit 1x x 4x 11 Nov 06 DIPA 14 Jun 07 No label 14 Jun 07 Kontrol Rendang K+LPS K+PWM K-,170,8,46 1,59 1,099 1,69 1,666,05 1,55,85 1,44 1,08,8 1,41 1,58 1,99 1,745 1,788 1,88 0,78 1,4,777 1,478 1,0,161 1,61 1,887 1,478 1,41 1,45 1,68 1,9 1,56 1,6 1,196 1,8 1,155 1,504 1,77 1,99 1,68 0,741 1,094 1,1 1,48 118
Lampiran 5. Contoh cara perhitungan untuk mendapatkan Indeks Stimulasi (I.S.) proliferasi limfosit * 11 November 06, pengenceran 1x Diketahui : Absorbansi sampel =,170 maka Absorbansi kontrol negatif = 1,904 I.S. = I.S. =,170 1,094 I.S. = 1,984 Abs ekstrak sampel Abs kontrol negatif 119
Lampiran 6. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 1x 11 Nov 006 1x DIPA 14 Jun 007 1x No Label 14 Jun 007 1x Kontrol Rendang 1x K+ 1x K- 1x Waktu pengamatan (jam) 0 1 4 5 0,10 0,1 0,18 0,18 0,1 0,17 0,117 0,119 0,119 0,118 0,14 0,11 0,11 0,10 0,15 0,14 0,10 0,1 0,16 0,17 0,1 0,1 0,19 0,11 0,119 0,16 0,1 0,1 0,10 0,1 0,114 0,118 0,1 0,116 0,14 0,1 0,17 0,17 0,18 0,14 0,17 0,141 0,14 0,159 0,164 0,17 0,171 0,179 0,16 0,16 0,18 0,180 0,117 0,19 0,166 0,164 0,168 0,170 0,18 0,17 0,14 0,11 0,11 0,18 0,1 0,1 0,106 0,10 0,117 0,114 0,116 0,1 0,105 0,104 0,11 0,108 0,108 0,115 0,108 0,119 0,14 0,14 0,147 0,160 0,095 0,101 0,107 0,106 0,111 0,115 0,105 0,1 0,1 0,18 0,151 0,16 0,107 0,105 0,11 0,110 0,117 0,11 10
Lampiran 7. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran x 11 Nov 006 x DIPA 14 Jun 007 x No Label 14 Jun 007 x Kontrol Rendang x Waktu pengamatan (jam) 0 1 4 5 0,17 0,19 0,145 0,151 0,150 0,155 0,110 0,11 0,11 0,11 0,115 0,116 0,108 0,108 0,108 0,109 0,11 0,11 0,114 0,11 0,15 0,11 0,114 0,114 0,16 0,11 0,109 0,10 0,1 0,17 0,114 0,10 0,1 0,1 0,1 0,1 0,140 0,17 0,17 0,1 0,14 0,140 0,10 0,115 0,118 0,11 0,117 0,10 0,119 0,11 0,116 0,108 0,11 0,11 0,1 0,11 0,141 0,10 0,158 0,144 0,119 0,108 0,117 0,111 0,11 0,114 0,14 0,14 0,146 0,146 0,149 0,15 11
Lampiran 8. Tabel data hasil pembacaan absorbansi uji hemolisis eritrosit pengenceran 4x 11 Nov 006 4x DIPA 14 Jun 007 4x No Label 14 Jun 007 4x Kontrol Rendang 4x Waktu pengamatan (jam) 0 1 4 5 0,1 0,16 0,159 0,00 0,00 0,6 0,109 0,107 0,106 0,111 0,110 0,108 0,110 0,101 0,10 0,10 0,106 0,106 0,115 0,107 0,11 0,11 0,111 0,115 0,111 0,106 0,111 0,115 0,106 0,106 0,099 0,100 0,10 0,104 0,104 0,104 0,109 0,119 0,116 0,11 0,119 0,10 0,106 0,118 0,11 0,111 0,118 0,14 0,108 0,109 0,11 0,106 0,11 0,115 0,109 0,101 0,106 0,10 0,106 0,100 0,104 0,10 0,106 0,105 0,106 0,10 0,109 0,11 0,116 0,11 0,116 0,11 1
Lampiran 9. Contoh cara perhitungan % hemolisis *sampel 11 Nov 06, pengenceran 1x jam ke-5 Diketahui: Absorbansi sampel = 0,17 Absorbansi Kontrol (-) = 0,115 % Hemolisis = = = 119,10% 1
Lampiran 10. Tabel data hasil pembacaan absorbansi pengukuran kapasitas antioksidan (metode DPPH) 11 Nov 06 DIPA 14 Jun 07 No label 14 Jun 07 Kontrol Rendang Absorbansi 1x x 4x 1,190 1,05 1,00 1,19 1,00 1,04 1,050 1,00 1,00 1,051 1,0 1,0 1,060 1,055 1,045 1,060 1,057 1,040 0,995 1,05 1,045 0,996 1,05 1,044 14
Lampiran 11. Contoh perhitungan untuk mendapatkan % kapasitas antioksidan tersisa *sampel 11 Nov 06 pengenceran 1x ulangan 1 Diketahui: Abs sampel: 1,190 Abs kontrol (-): 1,50 maka % Kapasitas Antioksidan tersisa = = = 11,85% Kapasitas ini setara dengan kapasitas antioksidan vitamin C (x) sebesar: y = 1,7 0,0011x 1,190 = 1,7 0,0011x x = 10,64 ppm 15
Lampiran 1. Tabel data hasil pembacaan absorbansi pengukuran kadar Malonaldehida 11 Nov 06 DIPA 14 Jun 07 No label 14 Jun 07 Kontrol Rendang Absorbansi 1x x 4x 0,458 0,50 0,170 0,4 0,8 0,17 0,464 0,8 0,166 0,70 0,07 0,110 0,46 0,70 0,180 0,58 0,69 0,1 0,54 0,40 0,0 0,56 0,10 0,5 16
Lampiran 1. Contoh perhitungan untuk mendapatkan kadar malonaldehida *sampel 11 Nov 06 pengenceran 1x ulangan 1 Diketahui : Abs sampel : 0,458 (y) Dengan memasukkan nilai y = 0,458 ke persamaan y= 0,005x + 0,117 maka y = 0,005x + 0,117 0,458 = 0,005x + 0,117 0,408 = 0,005x x = 16, pmol/ml 17
Lampiran 14. Hasil analisis statistik uji proliferasi limfosit pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors N 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM 1 7 7 7 Dependent Variable: Absorbansi Tests of Between-Subjects Effects Source Type III Sum of Squares Df Mean Square F Sig. Model 44,445(a) 9 4,98 4,894,000,754 6,459 4,080,018,660,0,9,09 Error 1,50 1,11 Total 45,795 1 a R Squared =,971 (Adjusted R Squared =,948) Post Hoc Tests 18
Multiple Comparisons Dependent Variable: Absorbansi Tukey HSD (I) 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM (J) DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM 11 Nov 06 Kontrol_R Ktrl Std LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Ktrl Std LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R LPS NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std NL14Jun PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS PWM 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun Based on observ ed means. *. The mean diff erence is signif icant at the,05 lev el. Mean Dif f erence 95% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound -,8967,7871,99-1,4818,66885,196,7871,989 -,7618 1,15485,4500,7871,657 -,5065 1,4105,9900,7871,76 -,5595 1,575 -,6567,7871,4-1,58418,85,47,7871,965 -,71118 1,0585,8967,7871,99 -,66885 1,4818,48600,7871,586 -,475 1,4445,74167,7871,177 -,1685 1,70018,68867,7871,5 -,6985 1,64718 -,600,7871,871-1,945,65,5700,7871,48 -,415 1,4955 -,196,7871,989-1,15485,7618 -,48600,7871,586-1,4445,475,5567,7871,959 -,7085 1,1418,067,7871,987 -,75585 1,16118 -,800,7871,11-1,7805,165,05100,7871 1,000 -,9075 1,0095 -,4500,7871,657-1,4105,5065 -,74167,7871,177-1,70018,1685 -,5567,7871,959-1,1418,7085 -,0500,7871 1,000-1,0115,9055-1,07767*,7871,04 -,0618 -,11915 -,0467,7871,986-1,1618,7585 -,9900,7871,76-1,575,5595 -,68867,7871,5-1,64718,6985 -,067,7871,987-1,16118,75585,0500,7871 1,000 -,9055 1,0115-1,0467*,7871,0-1,9818 -,06615 -,15167,7871,997-1,11018,80685,6567,7871,4 -,85 1,58418,600,7871,871 -,65 1,945,800,7871,11 -,165 1,7805 1,07767*,7871,04,11915,0618 1,0467*,7871,0,06615 1,9818,8700,7871,08 -,0855 1,815 -,47,7871,965-1,0585,71118 -,5700,7871,48-1,4955,415 -,05100,7871 1,000-1,0095,9075,0467,7871,986 -,7585 1,1618,15167,7871,997 -,80685 1,11018 -,8700,7871,08-1,815,0855 19
Homogeneous Subsets Tukey HSD Absorbansi Subset N 1 Ktrl Std 1,00000 LPS 1,0500 PWM 1,0467 1,0467 Kontrol_R 1,5567 1,5567 11 Nov 06 1,4500 1,4500 DIPA14Jun 1,74167 1,74167 NL14Jun,07767 Sig.,177,08 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) =,11. a Uses Harmonic Mean Sample Size =,000. b Alpha =,05. 10
Lampiran 15. Hasil analisis statistik uji proliferasi limfosit semua sampel pada selang kepercayaan 99% Univariate Analysis of Variance Between-Subjects Factors 11 Nov 06 DIPA14Jun Kontrol_R Ktrl Std LPS NL14Jun PWM 1 7 7 7 Dependent Variable: Absorbansi N Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Model 44,445(a) 9 4,98 4,894,000,754 6,459 4,080,018,660,0,9,09 Error 1,50 1,11 Total 45,795 1 a R Squared =,971 (Adjusted R Squared =,948) 11
Lampiran 16. Hasil analisis statistik uji proliferasi limfosit sampel 11 Nov 06 pada berbagai pengenceran pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 11Nov06_1 11Nov06_ 11Nov06_4 1 N Dependent Variable: IndeksStimulasi Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Model 1,659(a) 5 4, 85,677,000,011,005,107,901 1,819,909 17,986,010 Error,0 4,051 Total 1,861 9 a R Squared =,991 (Adjusted R Squared =,979) 1
Lampiran 17. Hasil analisis statistik uji proliferasi limfosit sampel DIPA 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors N DIPA_1 DIPA_ DIPA_4 1 Dependent Variable: IndeksStimulasi Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Model 19,08(a) 5,84,477,00,447,4 1,890,64,08,019,159,858 Error,47 4,118 Total 19,681 9 a R Squared =,976 (Adjusted R Squared =,946) 1
Lampiran 18. Hasil analisis statistik uji proliferasi limfosit sampel No Label 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors N NL_1 NL_ NL_ 1 Dependent Variable: IndeksStimulasi Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Model 0,185(a) 5 4,07 47,989,001,041 1,00 1,11,00,18,09 1,089,419 Error,6 4,084 Total 0,51 9 a R Squared =,984 (Adjusted R Squared =,96) Post Hoc Tests Dependent Variable: IndeksStimulasi Tukey HSD Multiple Comparisons (I) NL_1 NL_ NL_ (J) NL_ NL_ NL_1 NL_ NL_1 NL_ Mean Diff erence 95% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 1,08967*,6815,0,4566 1,967 Based on observ ed means. *. The mean diff erence is significant at the,05 level.,905*,6815,040,061 1,7494-1,08967*,6815,0-1,967 -,4566 -,184,6815,75-1,084,65967 -,905*,6815,040-1,7494 -,061,184,6815,75 -,65967 1,084 14
Homogeneous Subsets IndeksStimulasi Tukey HSD Subset N 1 NL_,98800 NL_ 1,17 NL_1,07767 Sig.,75 1,000 Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares The error term is Mean Square(Error) =,084. a Uses Harmonic Mean Sample Size =,000. b Alpha =,05. 15
Lampiran 19. Hasil analisis statistik uji proliferasi limfosit sampel No Label 14 Juni 07 pada berbagai pengenceran pada selang kepercayaan 99% Univariate Analysis of Variance Between-Subjects Factors N NL_1 NL_ NL_ 1 Dependent Variable: IndeksStimulasi Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Model 0,185(a) 5 4,07 47,989,001,041 1,00 1,11,00,18,09 1,089,419 Error,6 4,084 Total 0,51 9 a R Squared =,984 (Adjusted R Squared =,96) 16
Lampiran 0. Hasil analisis statistik uji proliferasi limfosit sampel Kontrol Rendang pada berbagai pengenceran pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors N K_1 K_ K_ 1 Dependent Variable: IndeksStimulasi Tests of Between-Subjects Effects Source Type III Sum of Squares df Mean Square F Sig. Model 11,697(a) 5,9 89,0,000,085,04 5,47,076,15,076 9,69,01 Error,0 4,008 Total 11,79 9 a R Squared =,997 (Adjusted R Squared =,994) 17
Lampiran 1. Hasil analisis statistik uji hemolisis eritrosit sampel 11 Nov 06 pada jam ke-5 pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors N 1,00,00,00 1,00,00,00 Dependent Variable: Absorbansi Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig.,171 a 5,04,179,00,001,000,04,754,006,00,048,157,004 4,001,175 9 a. R Squared =,976 (Adjusted R Squared =,945) 18
Lampiran. Hasil analisis statistik uji hemolisis eritrosit sampel DIPA 14 Juni 007 pada jam ke-5 pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1,00,00,00 1,00,00,00 N Dependent Variable: Absorbansi Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig.,14 a 5,07 90,417,000,001,000 4,6,100 5,00E-005,50E-005,71,776,000 4 9,E-005,14 9 a. R Squared =,997 (Adjusted R Squared =,994) 19
Lampiran. Hasil analisis statistik uji hemolisis eritrosit sampel No Label 14 Juni 007 pada jam ke-5 pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1,00,00,00 4,00 1,00,00,00 N 4 4 4 Dependent Variable: Absorbansi Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig.,198 a 6,0 186,486,000,00,001,8,076,001,000 1,58,89,001 6,000,199 1 a. R Squared =,995 (Adjusted R Squared =,989) 140
Lampiran 4. Hasil analisis statistik uji hemolisis eritrosit sampel kontrol pada jam ke-5 pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1,00,00,00 1,00,00,00 N Dependent Variable: Absorbansi Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig.,16 a 5,0 14,475,000,00,00 6,895,051,000,000,769,5,001 4,000,16 9 a. R Squared =,994 (Adjusted R Squared =,987) 141
Lampiran 5. Hasil analisis statistik persen hemolisis eritrosit semua sampel pada jam ke-5 pada selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1,00,00,00 4,00 5,00 1,00,00,00 N 5 5 4 Dependent Variable: PersenHemo Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 157585,4 a 7 51,0 10,5,000 76,48 4 181,610,971,480 95,40 1147,715 6,14,09 109,665 7 187,095 158895,088 14 a. R Squared =,99 (Adjusted R Squared =,984) 14
Lampiran 6. Hasil analisis statistik uji kapasitas antioksidan DPPH semua sampel pada pengenceran 1x dan selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1 4 1 N 4 4 Dependent Variable: KapAntiox Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 561,977 a 5 71,95 167786,9,000 7,74 75,791 17850,719,000,017 1,017 4,00,18,01,004 561,990 8 a. R Squared = 1,000 (Adjusted R Squared = 1,000) Post Hoc Tests 14
Multiple Comparisons Dependent Variable: KapAntiox Tukey HSD (I) 1,00,00,00 4,00 (J),00,00 4,00 1,00,00 4,00 1,00,00 4,00 1,00,00,00 Mean Diff erence 95% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 14,500*,06516,000 14,056 14,844 Based on observ ed means. *. The mean diff erence is significant at the,05 level. 4,0750*,06516,000,7606 4,894 4,7800*,06516,000 4,4656 5,0944-14,500*,06516,000-14,844-14,056-10,4450*,06516,000-10,7594-10,106-9,7400*,06516,000-10,0544-9,456-4,0750*,06516,000-4,894 -,7606 10,4450*,06516,000 10,106 10,7594,7050*,06516,005,906 1,0194-4,7800*,06516,000-5,0944-4,4656 9,7400*,06516,000 9,456 10,0544 -,7050*,06516,005-1,0194 -,906 Homogeneous Subsets Tukey HSD a,b,00 4,00,00 1,00 Sig. KapAntiox N 1 4 11,7400 1,4800 Subset,1850 6,600 1,000 1,000 1,000 1,000 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) =,004. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,05. 144
Lampiran 7. Hasil analisis statistik uji kapasitas antioksidan DPPH semua sampel pada pengenceran 1x dan selang kepercayaan 99% Univariate Analysis of Variance Between-Subjects Factors 1,00,00,00 4,00 1,00,00 N 4 4 Dependent Variable: KapAntiox Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 561,977 a 5 71,95 167786,9,000 7,74 75,791 17850,719,000,017 1,017 4,00,18,01,004 561,990 8 a. R Squared = 1,000 (Adjusted R Squared = 1,000) Post Hoc Tests 145
Multiple Comparisons Dependent Variable: KapAntiox Tukey HSD (I) 1,00,00,00 4,00 (J),00,00 4,00 1,00,00 4,00 1,00,00 4,00 1,00,00,00 Mean Diff erence 99% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 14,500*,06516,000 1,959 15,0807 Based on observ ed means. *. The mean diff erence is significant at the,01 level. 4,0750*,06516,000,514 4,657 4,7800*,06516,000 4,19 5,407-14,500*,06516,000-15,0807-1,959-10,4450*,06516,000-11,0057-9,884-9,7400*,06516,000-10,007-9,179-4,0750*,06516,000-4,657 -,514 10,4450*,06516,000 9,884 11,0057,7050*,06516,005,144 1,657-4,7800*,06516,000-5,407-4,19 9,7400*,06516,000 9,179 10,007 -,7050*,06516,005-1,657 -,144 Homogeneous Subsets Tukey HSD a,b,00 4,00,00 1,00 Sig. KapAntiox N 1 4 11,7400 1,4800 Subset,1850 6,600 1,000 1,000 1,000 1,000 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) =,004. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,01. 146
Lampiran 8. Hasil analisis statistik pengukuran kadar MDA semua sampel pada pengenceran 1x dan selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1 11Nov 06 DIPA Kontrol NoLabel N 4 4 Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 170545,84 a 5 4109,169 8,89,00 11,50 1 11,50,08,878 446,080 1148,69,771,1 14,50 414,507 171789,6 8 a. R Squared =,99 (Adjusted R Squared =,981) 147
Lampiran 9. Hasil analisis statistik pengukuran kadar MDA sampel 11 Nov 006 pada berbagai pengenceran dan selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors 1 11Nov_1 11Nov_ 11Nov_4 N Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 40490,76 a 4 101,68 645,579,00 4,560 1 4,560,04,76 1885,97 644,987 410,905,00 1,60 15,680 405,086 6 a. R Squared =,999 (Adjusted R Squared =,998) Post Hoc Tests 148
Multiple Comparisons Dependent Variable: kadarmda Tukey HSD (I) 11Nov_1 11Nov_ 11Nov_4 (J) 11Nov_ 11Nov_4 11Nov_1 11Nov_4 11Nov_1 11Nov_ Mean Diff erence 95% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 80,40000*,959798,004 57,0780 10,760 Based on observ ed means. *. The mean diff erence is significant at the,05 level. 109,60000*,959798,00 86,780 1,960-80,40000*,959798,004-10,760-57,0780 9,0000*,959798,0 5,8780 5,560-109,60000*,959798,00-1,960-86,780-9,0000*,959798,0-5,560-5,8780 Homogeneous Subsets Tukey HSD a,b 11Nov_4 11Nov_ 11Nov_1 Sig. kadarmda Subset N 1 1,5000 50,7000 11,1000 1,000 1,000 1,000 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 15,680. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,05. 149
Lampiran 0. Hasil analisis statistik pengukuran kadar MDA sampel 11 Nov 006 pada berbagai pengenceran dan selang kepercayaan 99% Univariate Analysis of Variance Between-Subjects Factors 11Nov_1 11Nov_ 11Nov_4 1 N Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 40490,76 a 4 101,68 645,579,00 1885,97 644,987 410,905,00 4,560 1 4,560,04,76 1,60 15,680 405,086 6 a. R Squared =,999 (Adjusted R Squared =,998) Post Hoc Tests 150
Multiple Comparisons Dependent Variable: kadarmda Tukey HSD (I) 11Nov_1 11Nov_ 11Nov_4 (J) 11Nov_ 11Nov_4 11Nov_1 11Nov_4 11Nov_1 11Nov_ Mean Diff erence 99% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 80,40000*,959798,004 7,1464 1,6558 Based on observ ed means. *. The mean diff erence is significant at the,01 level. 109,60000*,959798,00 56,464 16,8558-80,40000*,959798,004-1,6558-7,1464 9,0000,959798,0-4,0558 8,4558-109,60000*,959798,00-16,8558-56,464-9,0000,959798,0-8,4558 4,0558 Homogeneous Subsets Tukey HSD a,b 11Nov_4 11Nov_ 11Nov_1 Sig. kadarmda N 1 1,5000 50,7000 Subset 11,1000,0 1,000 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 15,680. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,01. 151
Lampiran 1. Hasil analisis statistik pengukuran kadar MDA sampel DIPA 14 Juni 007 pada berbagai pengenceran dan selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors DIPA_1 DIPA_ DIPA_4 1 N Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 5455,6 a 4 886,815 1,97,005 186,10 619,105 149,501,007 176,65 1 176,65 0,818,01 8,850 41,45 558,11 6 a. R Squared =,998 (Adjusted R Squared =,99) Post Hoc Tests 15
Multiple Comparisons Dependent Variable: kadarmda Tukey HSD (I) DIPA_1 DIPA_ DIPA_4 (J) DIPA_ DIPA_4 DIPA_1 DIPA_4 DIPA_1 DIPA_ Mean Diff erence 95% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 68,80000* 6,461,016 0,88574 106,7146 Based on observ ed means. *. The mean diff erence is significant at the,05 level. 110,16000* 6,461,006 7,4574 148,0746-68,80000* 6,461,016-106,7146-0,88574 41,6000* 6,461,04,44574 79,746-110,16000* 6,461,006-148,0746-7,4574-41,6000* 6,461,04-79,746 -,44574 Homogeneous Subsets Tukey HSD a,b DIPA_4 DIPA_ DIPA_1 Sig. kadarmda Subset N 1 9,76000 51,1000 119,9000 1,000 1,000 1,000 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 41,45. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,05. 15
Lampiran. Hasil analisis statistik pengukuran kadar MDA sampel DIPA 14 Juni 007 pada berbagai pengenceran dan selang kepercayaan 99% Univariate Analysis of Variance Between-Subjects Factors 1 DIPA_1 DIPA_ DIPA_4 N Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 5455,6 a 4 886,815 1,97,005 176,65 1 176,65 0,818,01 186,10 619,105 149,501,007 8,850 41,45 558,11 6 a. R Squared =,998 (Adjusted R Squared =,99) Post Hoc Tests Dependent Variable: kadarmda Tukey HSD Multiple Comparisons (I) DIPA_1 DIPA_ DIPA_4 (J) DIPA_ DIPA_4 DIPA_1 DIPA_4 DIPA_1 DIPA_ Mean Diff erence 99% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 68,80000 6,461,016-17,75804 155,5804 Based on observ ed means. *. The mean diff erence is significant at the,01 level. 110,16000* 6,461,006,60196 196,71804-68,80000 6,461,016-155,5804 17,75804 41,6000 6,461,04-45,19804 17,91804-110,16000* 6,461,006-196,71804 -,60196-41,6000 6,461,04-17,91804 45,19804 154
Homogeneous Subsets Tukey HSD a,b DIPA_4 DIPA_ DIPA_1 Sig. kadarmda Subset N 1 9,76000 51,1000 51,1000 119,9000,04,016 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 41,45. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,01. 155
Lampiran. Hasil analisis statistik pengukuran kadar MDA sampel No Label 14 Juni 007 pada berbagai pengenceran dan selang kepercayaan 95% Univariate Analysis of Variance Between-Subjects Factors NLabel_1 NLabel_ NLabel_4 1 N Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 615,585 a 4 15788,96 175,84,006 14914,9 7457,147 8,054,01 115,707 1 115,707 1,849,070 179,57 89,787 6,158 6 a. R Squared =,997 (Adjusted R Squared =,991) Post Hoc Tests 156
Multiple Comparisons Dependent Variable: kadarmda Tukey HSD (I) NLabel_1 NLabel_ NLabel_4 (J) NLabel_ NLabel_4 NLabel_1 NLabel_4 NLabel_1 NLabel_ Mean Diff erence 95% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 7,0000* 9,47558,01 16,8166 18,0184 Based on observ ed means. *. The mean diff erence is significant at the,05 level. 11,40000* 9,47558,011 65,58166 177,184-7,0000* 9,47558,01-18,0184-16,8166 49,0000 9,47558,06-6,6184 105,0184-11,40000* 9,47558,011-177,184-65,58166-49,0000 9,47558,06-105,0184 6,6184 Homogeneous Subsets Tukey HSD a,b NLabel_4 NLabel_ NLabel_1 Sig. kadarmda N 1 1,7000 80,9000 Subset 15,1000,06 1,000 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 89,787. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,05. 157
Lampiran 4. Hasil analisis statistik pengukuran kadar MDA sampel No Label 14 Juni 007 pada berbagai pengenceran dan selang kepercayaan 99% Univariate Analysis of Variance Between-Subjects Factors 1 NL_1 NL_ NL_4 N Dependent Variable: kadarmda Source Model Error Total Tests of Between-Subjects Effects Type III Sum of Squares df Mean Square F Sig. 615,585 a 4 15788,96 175,84,006 115,707 1 115,707 1,849,070 14914,9 7457,147 8,054,01 179,57 89,787 6,158 6 a. R Squared =,997 (Adjusted R Squared =,991) Post Hoc Tests 158
Multiple Comparisons Dependent Variable: kadarmda Tukey HSD (I) NL_1 NL_ NL_4 (J) NL_ NL_4 NL_1 NL_4 NL_1 NL_ Based on observ ed means. Mean Diff erence 99% Confidence Interv al (I-J) Std. Error Sig. Lower Bound Upper Bound 7,0000 9,47558,01-55,95 199,695 11,40000 9,47558,011-6,095 48,895-7,0000 9,47558,01-199,695 55,95 49,0000 9,47558,06-78,95 176,695-11,40000 9,47558,011-48,895 6,095-49,0000 9,47558,06-176,695 78,95 Homogeneous Subsets Tukey HSD a,b NL_4 NL_ NL_1 Sig. kadarmda Subset N 1 1,7000 80,9000 15,1000,011 Means for groups in homogeneous subsets are display ed. Based on Ty pe III Sum of Squares The error term is Mean Square(Error) = 89,787. a. Uses Harmonic Mean Sample Size =,000. b. Alpha =,01. 159
Lampiran 5. Inform concern INFORM CONCERN PERNYATAAN KESEDIAAN MENJADI RESPONDEN PENELITIAN Saya yang bertanda tangan di bawah ini: Nama : Ari Try Purbayanto Jenis kelamin : Laki-laki Pekerjaan : Mahasiswa Ilmu dan Teknologi Pangan (ITP) NRP : F40570 Alamat : Jalan Raya Darmaga Gang Nurul Asman RT 01 RW Darmaga Bogor Telepon : 085697486586 Menyatakan dalam keadaan sehat dan bersedia atau tidak berkeberatan untuk dilakukan pengambilan darah untuk keperluan penelitian yang berjudul Kapasitas Antioksidan, Proliferasi Limfosit dan Hemolisis Eritrosit Manusia Ekstrak Rendang Iradiasi Dosis Tinggi. Penelitian ini dilakukan dalam rangka penulisan skripsi oleh Kamalita Pertiwi/ NRP F40500. Pengambilan darah dilakukan di Klinik Farfa Darmaga- Bogor pada bulan Februari 008 - April 009. Demikian keterangan ini dibuat untuk digunakan seperlunya. Petugas Pengambil Darah Bogor, Februari 008 Responden Neng Ari Try Purbayanto 160
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