LAMPIRAN 1. Data hasil penelitian A. Data Standart Plate Count (SPC) No. formula waktu inkubasi hasil SPC 1 K 0 7 x 10 6 2 K 0 1,1 x 10 5 3 K 0 5,5 x 10 6 4 K 7 1,2 x 10 21 5 K 7 3,1 x 10 16 6 K 7 7,1 x 10 15 7 K 14 5,2 x 10 23 8 K 14 3,2 x 10 22 9 K 14 3,5 x 10 24 10 M1 0 >3,0 x 10 11 (347 x 10 9 ) 11 M1 0 1,7 x 10 11 12 M1 0 1,7 x 10 11 13 M1 7 2,2 x 10 20 14 M1 7 1 x 10 22 15 M1 7 9,8 x 10 21 16 M1 14 2,2 x 10 26 17 M1 14 9,4 x 10 24 18 M1 14 >3 x 10 27 (318 x 10 25 ) 19 M2 0 2 x 10 11 20 M2 0 2,7 x 10 11 21 M2 0 2,9 x 10 11 22 M2 7 2 x 10 22 23 M2 7 9,1 x 10 21 24 M2 7 2,2 x 10 20 25 M2 14 1,2 x 10 25 26 M2 14 >3 x 10 25 (302 x 10 25 ) 27 M2 14 1,5 x 10 27 28 M3 0 4,7 x 10 9 29 M3 0 7,3 x 10 9 30 M3 0 1,1 x 10 11
31 M3 7 1,7 x 10 20 32 M3 7 2,7 x 10 21 33 M3 7 1,1 x 10 20 34 M3 14 2,3 x 10 25 35 M3 14 2,5 x 10 25 36 M3 14 2,1 x 10 27
B. Data nilai oil sludge residu
C. Data oil sludge residu Residu SLUDGE WAKTU ISOLAT 0,31 0 K 0,32 0 K 0,32 0 K 0,28 7 K 0,29 7 K 0,28 7 K 0,22 14 K 0,24 14 K 0,25 14 K 0,3 0 M1 0,31 0 M1 0,31 0 M1 0,24 7 M1 0,22 7 M1 0,23 7 M1 0,15 14 M1 0,14 14 M1 0,14 14 M1 0,27 0 M2 0,24 0 M2 0,25 0 M2 0,17 7 M2 0,15 7 M2 0,16 7 M2 0,13 14 M2 0,14 14 M2 0,14 14 M2 0,23 0 M3 0,21 0 M3 0,21 0 M3 0,17 7 M3 0,17 7 M3 0,18 7 M3 0,13 14 M3 0,13 14 M3 0,14 14 M3
D. Data oil sludge yang telah terdegradasi WAKTU ISOLAT SLUDGE DEGRADASI 0 K 0,01 0 K 0 0 K 0 7 K 0,04 7 K 0,03 7 K 0,04 14 K 0,1 14 K 0,08 14 K 0,07 0 M1 0,02 0 M1 0,01 0 M1 0,01 7 M1 0,08 7 M1 0,1 7 M1 0,09 14 M1 0,17 14 M1 0,18 14 M1 0,18 0 M2 0,05 0 M2 0,08 0 M2 0,07 7 M2 0,15 7 M2 0,17 7 M2 0,16 14 M2 0,19 14 M2 0,18 14 M2 0,18 0 M3 0,09 0 M3 0,11 0 M3 0,11 7 M3 0,15 7 M3 0,15 7 M3 0,14 14 M3 0,19 14 M3 0,19 14 M3 0,18
E. Data ph, oil sludge sisa, oil sludge yang terdegradasi dan log TPC masing-masing konsorsium bakteri. Kontrol 0 hari 7 hari 14 hari Persentase degradasi (%) 0 12,5 25 Log TPC 6,2 17,8 23,6 PH 7 6,1 6,7 Persentase residu (%) 100 87,5 75 Konsorsium M1 0 hari 7 hari 14 hari Persentase degradasi (%) 3,12 28,12 56,25 Log TPC 11,3 21,8 25,6 PH 7 6,6 7 Persentase residu (%) 96,88 71,88 43,75 Konsorsium M2 0 hari 7 hari 14 hari Persentase degradasi (%) 21,87 50 56,25 Log TPC 10,7 21,5 26,2 PH 7 6,3 6,8 Persentase residu (%) 78,13 50 43,75 Konsorsium M3 0 hari 7 hari 14 hari Persentase degradasi (%) 31,25 46,87 59,37 Log TPC 10,2 20,6 26 PH 7 6,1 6,9 Persentase residu (%) 68,75 53,13 40,63
2. Hasil uji statistik A. Hasil analisis statistik pada TPC a. Hasil uji normalitas menggunakan One-Sample Kolmogorov-Smirnov Test One-Sample Kolmogorov-Smirnov Test FORMULA TPC waktu_inkubasi N 36 36 36 Normal Parameters a Mean 5.5000 18.4731 7.0000 Std. Deviation 3.50102 6.94053 5.79655 Most Extreme Differences Absolute.096.201.220 Positive.096.174.220 Negative -.096 -.201 -.220 Kolmogorov-Smirnov Z.574 1.206 1.318 Asymp. Sig. (2-tailed).896.109.062 a. Test distribution is Normal. b. Hasil uji homogenitas menggunakan Levene's Test Levene's Test of Equality of Error Variances a Dependent Variable:TPC F df1 df2 Sig. 3.765 11 24.003 Tests the null hypothesis that the error variance of the dependent variable is equal across groups. a. Design: Intercept + FORMULA + waktu_inkubasi + FORMULA * waktu_inkubasi c. Hasil uji Brown-Forsythe Robust Tests of Equality of Means TPC Statistic a df1 df2 Sig. Brown-Forsythe 4.185 2 11.000.045 a. Asymptotically F distributed.
d. Hasil uji Games-Howell TPC Games-Howell (I) FORMU LA (J) FORMU LA Multiple Comparisons Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval Lower Bound Upper Bound KH0 KH7-11.59333 1.74304.081-25.8794 2.6928 KH14-17.38333 *.83353.000-22.2213-12.5454 M1H0-5.12667.59818.065-10.9465.6931 M1H7-15.57667 *.62779.003-20.6765-10.4768 M1H14-19.39667 *.74803.000-23.8849-14.9084 M2H0-4.52000.86596.072-9.5620.5220 M2H7-15.33000 *.84579.001-20.2415-10.4185 M2H14-20.09333 *.88367.000-25.2585-14.9282 M3H0-3.98667.73049.071-8.4526.4793 M3H7-14.36000 *.73204.001-18.8270-9.8930 M3H14-19.83000 *.87857.000-24.9589-14.7011 KH7 KH0 11.59333 1.74304.081-2.6928 25.8794 KH14-5.79000 1.74318.343-20.0736 8.4936 M1H0 6.46667 1.64369.293-10.5137 23.4470 M1H7-3.98333 1.65469.581-20.5549 12.5882 M1H14-7.80333 1.70395.200-22.9160 7.3093 M2H0 7.07333 1.75891.234-6.9409 21.0876 M2H7-3.73667 1.74907.652-17.9156 10.4423 M2H14-8.50000 1.76770.158-22.3774 5.3774 M3H0 7.60667 1.69632.211-7.6976 22.9110 M3H7-2.76667 1.69699.826-18.0537 12.5204 M3H14-8.23667 1.76516.170-22.1527 5.6794 KH14 KH0 17.38333 *.83353.000 12.5454 22.2213 KH7 5.79000 1.74318.343-8.4936 20.0736 M1H0 12.25667 *.59859.011 6.4324 18.0810 M1H7 1.80667.62818.435-3.2977 6.9110 M1H14-2.01333.74836.437-6.5044 2.4777 M2H0 12.86333 *.86624.002 7.8200 17.9067 M2H7 2.05333.84608.521-2.8597 6.9664 M2H14-2.71000.88395.327-7.8763 2.4563 M3H0 13.39667 *.73083.001 8.9277 17.8657 M3H7 3.02333.73238.161-1.4467 7.4934 M3H14-2.44667.87885.403-7.5768 2.6835 M1H0 KH0 5.12667.59818.065 -.6931 10.9465 KH7-6.46667 1.64369.293-23.4470 10.5137 KH14-12.25667 *.59859.011-18.0810-6.4324
M1H7-10.45000 *.24012.000-12.2018-8.6982 M1H14-14.27000 *.47232.004-18.6865-9.8535 M2H0.60667.64298.983-5.7059 6.9193 M2H7-10.20333 *.61554.017-16.2144-4.1922 M2H14-14.96667 *.66664.009-21.5385-8.3949 M3H0 1.14000.44402.529-2.9563 5.2363 M3H7-9.23333 *.44657.009-13.3585-5.1081 M3H14-14.70333 *.65987.009-21.2010-8.2057 M1H7 KH0 15.57667 *.62779.003 10.4768 20.6765 KH7 3.98333 1.65469.581-12.5882 20.5549 KH14-1.80667.62818.435-6.9110 3.2977 M1H0 10.45000 *.24012.000 8.6982 12.2018 M1H14-3.82000 *.50930.047-7.5582 -.0818 M2H0 11.05667 *.67061.010 5.4602 16.6532 M2H7.24667.64435 1.000-5.0452 5.5385 M2H14-4.51667.69333.090-10.3766 1.3433 M3H0 11.59000 *.48317.002 8.1447 15.0353 M3H7 1.21667.48551.520-2.2546 4.6880 M3H14-4.25333.68682.100-10.0378 1.5311 M1H14 KH0 19.39667 *.74803.000 14.9084 23.8849 KH7 7.80333 1.70395.200-7.3093 22.9160 KH14 2.01333.74836.437-2.4777 6.5044 M1H0 14.27000 *.47232.004 9.8535 18.6865 M1H7 3.82000 *.50930.047.0818 7.5582 M2H0 14.87667 *.78432.001 10.0676 19.6857 M2H7 4.06667.76199.074 -.5422 8.6756 M2H14 -.69667.80383.994-5.6869 4.2935 M3H0 15.41000 *.63158.000 11.7354 19.0846 M3H7 5.03667 *.63337.017 1.3532 8.7201 M3H14 -.43333.79822 1.000-5.3709 4.5042 M2H0 KH0 4.52000.86596.072 -.5220 9.5620 KH7-7.07333 1.75891.234-21.0876 6.9409 KH14-12.86333 *.86624.002-17.9067-7.8200 M1H0 -.60667.64298.983-6.9193 5.7059 M1H7-11.05667 *.67061.010-16.6532-5.4602 M1H14-14.87667 *.78432.001-19.6857-10.0676 M2H7-10.81000 *.87804.003-15.9121-5.7079 M2H14-15.57333 *.91459.001-20.8859-10.2608 M3H0.53333.76761.999-4.2799 5.3465 M3H7-9.84000 *.76908.005-14.6519-5.0281 M3H14-15.31000 *.90966.001-20.5919-10.0281 M2H7 KH0 15.33000 *.84579.001 10.4185 20.2415 KH7 3.73667 1.74907.652-10.4423 17.9156 KH14-2.05333.84608.521-6.9664 2.8597
M1H0 10.20333 *.61554.017 4.1922 16.2144 M1H7 -.24667.64435 1.000-5.5385 5.0452 M1H14-4.06667.76199.074-8.6756.5422 M2H0 10.81000 *.87804.003 5.7079 15.9121 M2H14-4.76333.89551.068-9.9809.4542 M3H0 11.34333 *.74477.002 6.7462 15.9405 M3H7.97000.74629.937-3.6272 5.5672 M3H14-4.50000.89048.080-9.6835.6835 M2H14 KH0 20.09333 *.88367.000 14.9282 25.2585 KH7 8.50000 1.76770.158-5.3774 22.3774 KH14 2.71000.88395.327-2.4563 7.8763 M1H0 14.96667 *.66664.009 8.3949 21.5385 M1H7 4.51667.69333.090-1.3433 10.3766 M1H14.69667.80383.994-4.2935 5.6869 M2H0 15.57333 *.91459.001 10.2608 20.8859 M2H7 4.76333.89551.068 -.4542 9.9809 M3H0 16.10667 *.78753.001 11.0992 21.1141 M3H7 5.73333 *.78897.033.7284 10.7383 M3H14.26333.92654 1.000-5.1148 5.6414 M3H0 KH0 3.98667.73049.071 -.4793 8.4526 KH7-7.60667 1.69632.211-22.9110 7.6976 KH14-13.39667 *.73083.001-17.8657-8.9277 M1H0-1.14000.44402.529-5.2363 2.9563 M1H7-11.59000 *.48317.002-15.0353-8.1447 M1H14-15.41000 *.63158.000-19.0846-11.7354 M2H0 -.53333.76761.999-5.3465 4.2799 M2H7-11.34333 *.74477.002-15.9405-6.7462 M2H14-16.10667 *.78753.001-21.1141-11.0992 M3H7-10.37333 *.61255.001-13.9288-6.8179 M3H14-15.84333 *.78181.001-20.7945-10.8922 M3H7 KH0 14.36000 *.73204.001 9.8930 18.8270 KH7 2.76667 1.69699.826-12.5204 18.0537 KH14-3.02333.73238.161-7.4934 1.4467 M1H0 9.23333 *.44657.009 5.1081 13.3585 M1H7-1.21667.48551.520-4.6880 2.2546 M1H14-5.03667 *.63337.017-8.7201-1.3532 M2H0 9.84000 *.76908.005 5.0281 14.6519 M2H7 -.97000.74629.937-5.5672 3.6272 M2H14-5.73333 *.78897.033-10.7383 -.7284 M3H0 10.37333 *.61255.001 6.8179 13.9288 M3H14-5.47000 *.78326.037-10.4190 -.5210 M3H14 KH0 19.83000 *.87857.000 14.7011 24.9589 KH7 8.23667 1.76516.170-5.6794 22.1527 KH14 2.44667.87885.403-2.6835 7.5768
M1H0 14.70333 *.65987.009 8.2057 21.2010 M1H7 4.25333.68682.100-1.5311 10.0378 M1H14.43333.79822 1.000-4.5042 5.3709 M2H0 15.31000 *.90966.001 10.0281 20.5919 M2H7 4.50000.89048.080 -.6835 9.6835 M2H14 -.26333.92654 1.000-5.6414 5.1148 M3H0 15.84333 *.78181.001 10.8922 20.7945 M3H7 5.47000 *.78326.037.5210 10.4190 *. The mean difference is significant at the 0.05 level. HASIL GAMES_HOWELL TPC 0 1 2 3 4 5 6 7 8 9 10 11 0 TS S TS S S TS S S TS S S 1 TS TS TS TS TS TS TS TS TS TS TS 2 S TS TS TS TS S TS TS S TS TS 3 TS TS TS S S TS TS S TS S S 4 S TS TS S S S TS TS S TS TS 5 S TS TS S S S TS TS S S TS 6 TS TS S TS S S S S TS S S 7 S TS TS S TS TS S TS S TS TS 8 S TS TS S TS TS S TS S S TS 9 TS TS S TS S S TS S S S S 10 S TS TS S TS S S TS S S S 11 S TS TS S TS TS S TS TS S S B. Hasil analisis statistik pada Residu oil sludge a. Hasil uji normalitas menggunakan One-Sample Kolmogorov-Smirnov Test One-Sample Kolmogorov-Smirnov Test waktu_inkubasi SLUDGE_RESI DU FORMULA N 36 36 36 Normal Parameters a Mean 7.0000.2158 5.5000 Std. Deviation 5.79655.06478 3.50102 Most Extreme Differences Absolute.220.149.096 Positive.220.149.096 Negative -.220 -.093 -.096 Kolmogorov-Smirnov Z 1.318.896.574 Asymp. Sig. (2-tailed).062.399.896 a. Test distribution is Normal.
b. Hasil uji homogenitas menggunakan Levene's Test Levene's Test of Equality of Error Variances a Dependent Variable:SLUDGE_RESIDU F df1 df2 Sig. 1.243 11 24.314 Tests the null hypothesis that the error variance of the dependent variable is equal across groups. a. Design: Intercept + FORMULA + waktu_inkubasi + FORMULA * waktu_inkubasi c. Hasil uji ANOVA 2 arah Between-Subjects Factors Value Label N FORMULA 1 K 9 2 M1 9 3 M2 9 4 M3 9 WAKTU 1 0 12 2 7 12 3 14 12 Tests of Between-Subjects Effects Dependent Variable:SLUDGE_RESIDU Source Type III Sum of Squares df Mean Square F Sig. Corrected Model.145 a 11.013 152.877.000 Intercept 1.677 1 1.677 1.948E4.000 FORMULA.071 9.008 91.344.000 waktu_inkubasi.000 0... FORMULA * waktu_inkubasi.000 0... Error.002 24 8.611E-5 Total 1.824 36 Corrected Total.147 35 a. R Squared =,986 (Adjusted R Squared =,979)
d. Hasil uji Duncan Duncan FORM ULA N SLUDGE_RESIDU M3 9.1744 Subset 1 2 3 M2 9.1833 M1 9.2267 K 9.2789 Sig..053 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005. Duncan WAKT U N SLUDGE_RESIDU 14 12.1625 Subset 1 2 3 7 12.2117 0 12.2733 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005. Duncan INTER AKSI N M314 3.1333 M214 3.1367 M114 3.1433 M27 3.1600 M37 3.1733 SLUDGE_RESIDU Subset 1 2 3 4 5 6 7 M30 3.2167 M17 3.2300.2300 K14 3.2367 M20 3.2533 K7 3.2833 M10 3.3067 K0 3.3167
Sig..224.091.091.388 1.000 1.000.199 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005. C. Hasil analisis statistik pada Oil sludge yang telah terdegradasi a. Hasil uji normalitas menggunakan One-Sample Kolmogorov-Smirnov Test One-Sample Kolmogorov-Smirnov Test FORMULA WAKTU SLUDGE_DEG RADASI N 36 36 36 Normal Parameters a Mean 2.50 2.0000.1042 Std. Deviation 1.134.82808.06478 Most Extreme Differences Absolute.170.220.149 Positive.170.220.093 Negative -.170 -.220 -.149 Kolmogorov-Smirnov Z 1.022 1.318.896 Asymp. Sig. (2-tailed).247.062.399 a. Test distribution is Normal. b. Hasil uji homogenitas menggunakan Levene's Test Levene's Test of Equality of Error Variances a Dependent Variable:SLUDGE_DEGRADASI F df1 df2 Sig. 1.243 11 24.314 Tests the null hypothesis that the error variance of the dependent variable is equal across groups. a. Design: Intercept + FORMULA + WAKTU + FORMULA * WAKTU
c. Hasil uji ANOVA 2 arah Between-Subjects Factors Value Label N FORMULA 1 K 9 2 M1 9 3 M2 9 4 M3 9 WAKTU 1 0 12 2 7 12 3 14 12 Tests of Between-Subjects Effects Dependent Variable:SLUDGE_DEGRADASI Source Type III Sum of Squares df Mean Square F Sig. Corrected Model.145 a 11.013 152.877.000 Intercept.391 1.391 4.536E3.000 FORMULA.062 3.021 239.086.000 WAKTU.074 2.037 429.774.000 FORMULA * WAKTU.009 6.002 17.473.000 Error.002 24 8.611E-5 Total.538 36 Corrected Total.147 35 a. R Squared =,986 (Adjusted R Squared =,979) d. Hasil uji Duncan Duncan FORM ULA N SLUDGE_DEGRADASI Subset 1 2 3 K 9.0411 M1 9.0933 M2 9.1367 M3 9.1456 Sig. 1.000 1.000.053 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.
Duncan WAKT U N SLUDGE_DEGRADASI 0 12.0467 Subset 1 2 3 7 12.1083 14 12.1575 Sig. 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005. Duncan INTER AKSI N K0 3.0033 M10 3.0133 SLUDGE_DEGRADASI Subset 1 2 3 4 5 6 7 K7 3.0367 M20 3.0667 K14 3.0833 M17 3.0900.0900 M30 3.1033 M37 3.1467 M27 3.1600 M114 3.1767 M214 3.1833 M314 3.1867 Sig..199 1.000 1.000.388.091.091.224 Means for groups in homogeneous subsets are displayed. Based on observed means. The error term is Mean Square(Error) = 8,61E-005.
3. Alat dan Bahan Penelitian a. Alat-alat penelitian 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Keterangan: 1. Pipet tip 2. Sendok media 3. Micropipet 4. Pipet ukur 5. Pipetor 6. Botol kultur 7. Bunsen 8. Gelas ukur 10 ml 9. Botol aquades 10. Gelas ukur 500 ml 11. Labu gojok 12. Alumunium foil 13. Kapas 14. Masker face 15. Pematik api 16. Digi counter 17. Gelas beker 18. Petri disk Timbangan analitik Spektrofotometer Hot plate
Timbangan analitik Coloni Counter Autoclave Shaker Kulkas b. Bahan-bahan penelitian Foto NB Keterangan Spirtus Alkohol 70 % Oil Sludge N-Heksan Pewarna Gram N-heksan
4. Perlakuan dan Inkubasi Kontrol pada masa inkubasi 7 hari Konsorsium M1 pada masa inkubasi 7 hari Hasil Pour plate pada perlakuan kontrol setelah 7 hari inkubasi Hasil Pour plate pada konsorsium M3 setelah 14 hari inkubasi 5. Data Formulasi a. Data Isolat Penyusun Konsorsium No. Nama konsorsium Jumlah isolat bakteri Kode isolat bakteri 1. M1 7 jenis - S A2 A - M B1 C - O A2 G - O A1 E - S A1 A - O A2 E1 - O A2 E2 2. M2 5 jenis - SA2A - MB1C - OA2G - OA1E - SA1A
3. M3 3 jenis - SA2A - MB1C - OA2G 4. Kontrol Tanpa penambahan bakteri - b. Hasil Uji Menggunakan Microbact Identification Kit Setelah dilakukan identifikasi menggunakan Microbact Identification Kit 12A dan 12B, hasil identifikasi pada isolat bakteri yang digunakan adalah sebagai berikut: No Kode Isolat Hasil Uji Menggunakan Microbact Identification Kit 1 M B1 C Pseudomonas Stutzeri (dengan tingkat kesamaan 93,70%) 2 S A2 A Bacillus spsa2a (merujuk pada Bacillus cereus dengan tingkat kesamaan 71%) 3 OA2G Bacillus spoa2g (merujuk pada Bacillus stearothermophilus dengan tingkat kesamaan 65%) 4 OA1E Vibrio spoa1e ( merujuk pada Vibrio alginoluticus dengan tingkat kesamaan 94,97 %) c. Karakteristik makroskopis isolat bakteri yang digunakan adalah sebagai berikut: No. Kode Isolat Bentuk Koloni Warna Elevasi Tepi 1 SA2A Bulat Putih Flat Tidak rata 2 MB1C Bulat, Putih, Flat Rata besar bening 3 OA2G Bulat, Putih Flat Rata kecil 4 OA1E Bulat Putih, Flat Rata transparan 5 SA1A Bulat, Kecil Putih, Tengah ada titik Flat Rata 6 OA2E1 Bulat Transparan Cembung Rata 7 OA2E2 Bulat Putih Flat Rata
d. Foto hasil isolat No. Kode Isolat Foto Koloni Foto Mikroskop dengan perbesaran 1000 X 1 SA2A 2 MB1C 3 OA2G 4 OA1E 5 SA1A 6 OA2E1
7 OA2E2