Lampiran 1. Surat Keterangan Penelitian dari Laboratorium Parasitologi, Rumah Sakit Islam Sultan Agung Semarang 52
Lampiran 2. Surat Keterangan Penelitian dari Laboratorium Teknologi Farmasi Universitas Wahid Hasyim Semarang 53
Lampiran 3. Informed Concern 54
Lampiran 4. Dokumentasi Penelitian ph Meter Aspirator Alat Uji Daya Sebar Uji Homogenitas Sangkar Nyamuk Viskosimeter 55
Lampiran 4. Lanjutan Losion Minyak Atsiri Daun Rosemary Uji Daya Proteksi 56
Lampiran 5. Karakteristik Fisik Dan Aktivitas Repellent Losion Minyak Atsiri Daun Rosemary A. Hasil Uji Daya Sebar Losion Minyak Atsiri Daun Rosemary dengan Variasi Konsentrasi Gliserin Formula I II Luas Daya Sebar (cm^2) III IV V Ratarata SD SE F I 98.56 100.33 95.07 95.07 84.98 94.80 5.942 2.657 F II 74.69 73.17 70.91 75.46 73.93 73.63 1.745 0.780 F III 66.50 67.96 62.24 66.65 59.47 64.56 3.570 1.596 F IV 88.28 86.62 86.96 83.36 81.75 85.39 2.728 1.220 F V 31.38 27.72 30.30 31.09 22.24 28.55 3.810 1.704 Keterangan : FI : Formula losion dengan konsentrasi Gliserin 5,0% b/b FII : Formula losion dengan konsentrasi Gliserin 7.5% b/b FIII : Formula losion dengan konsentrasi Gliserin 10,0 % b/b FIV : Formula losion tanpa minyak rosemary (kontrol -) FV : Formula losion dengan kandungan DEET (kontrol +) B. Hasil Uji Viskositas Losion Minyak Atsiri Daun Rosemary dengan Variasi Konsentrasi Gliserin Formula I II Viskositas (dpas) III IV V Ratarata F I 9.50 9.00 10.00 10.50 9.50 9.70 0.570 0.255 F II 13.00 12.00 13.00 12.50 13.50 12.80 0.570 0.255 F III 14.00 14.50 15.00 15.50 14.00 14.60 0.652 0.292 F IV 11.00 11.50 12.00 10.50 11.00 11.20 0.570 0.255 F V 80.00 70.00 70.00 65.00 60.00 69.00 7.416 3.317 Keterangan : FI : Formula losion dengan konsentrasi Gliserin 5,0% b/b FII : Formula losion dengan konsentrasi Gliserin 7.5% b/b FIII : Formula losion dengan konsentrasi Gliserin 10,0 % b/b FIV : Formula losion tanpa minyak rosemary (kontrol -) FV : Formula losion dengan kandungan DEET (kontrol +) SD SE 57
Lampiran 5. Lanjutan C. Hasil Uji ph Losion Minyak Atsiri Daun Rosemary dengan Variasi Konsentrasi Gliserin Formula I II ph III IV V Ratarata F I 5.10 5.30 5.15 5.20 5.10 5.17 0.084 0.037 F II 5.40 5.50 5.37 5.40 5.50 5.43 0.061 0.027 F III 5.90 5.60 5.75 5.70 5.60 5.71 0.124 0.056 F IV 4.80 5.00 4.70 4.90 5.00 4.88 0.130 0.058 F V 6.00 5.80 6.20 5.80 6.10 5.98 0.179 0.080 Keterangan : FI : Formula losion dengan konsentrasi Gliserin 5,0% b/b FII : Formula losion dengan konsentrasi Gliserin 7.5% b/b FIII : Formula losion dengan konsentrasi Gliserin 10,0 % b/b FIV : Formula losion tanpa minyak rosemary (kontrol -) FV : Formula losion dengan kandungan DEET (kontrol +) SD SE 58
Lampiran 5. Lanjutan D. Rata-rata Daya Proteksi (%) pada Masing-masing Perlakuan Setiap Jam Formula Rata-rata Daya Proteksi (%) Jam Ke- 1 2 3 4 5 6 F I 1 88 83 82 80 79 78 2 86 86 80 81 80 76 3 87 84 81 79 78 78 4 87 80 82 79 79 77 5 87 82 80 81 79 76 Rata-rata 87 83 81 80 79 77 SD 0.707 2.236 1.000 1.000 0.707 1.000 SE 0.316 1.000 0.447 0.447 0.316 0.447 F II 1 70 68 65 65 64 62 2 69 67 66 63 63 62 3 68 67 64 63 63 61 4 70 68 63 62 63 60 5 68 65 62 62 62 60 Rata-rata 69 67 64 63 63 61 SD 1.000 1.225 1.581 1.225 0.707 1.000 SE 0.447 0.548 0.707 0.548 0.316 0.447 F III 1 63 56 57 56 54 46 2 61 55 55 56 53 47 3 59 58 54 55 52 44 4 59 57 55 54 50 45 5 58 59 54 54 51 43 Rata-rata 60 57 55 55 52 45 SD 2.000 1.581 1.225 1.000 1.581 1.581 SE 0.894 0.707 0.548 0.447 0.707 0.707 F IV 1 25 22 20 23 22 21 2 24 21 21 22 21 19 3 24 25 22 23 19 17 4 22 24 21 21 20 19 5 20 23 21 21 18 19 Rata-rata 23 23 21 22 20 19 SD 2.000 1.581 0.707 1.000 1.581 1.414 SE 0.894 0.707 0.316 0.447 0.707 0.632 F V 1 97 97 95 94 93 92 2 94 95 96 90 92 89 3 95 92 91 95 92 90 4 93 96 92 91 93 90 5 96 95 91 90 95 89 Rata-rata 95 95 93 92 93 90 SD 1.581 1.871 2.345 2.345 1.225 1.225 SE 0.707 0.837 1.049 1.049 0.548 0.548 Keterangan : FI : Formula losion dengan konsentrasi Gliserin 5,0% b/b FII : Formula losion dengan konsentrasi Gliserin 7.5% b/b FIII : Formula losion dengan konsentrasi Gliserin 10,0 % b/b FIV : Formula losion tanpa minyak rosemary (kontrol -) FV : Formula losion dengan kandungan DEET (kontrol +) 59
Lampiran 6. Hasil Analisis Daya Sebar Losion Rosemary Minyak Atsiri Daun Normalitas - Daya Sebar REPLIKASI Kolmogorov-Smirnova Shapiro-Wilk Statistic df Sig. Statistic df Sig. DYSEBAR F1.318 5.109.863 5.240 F2.196 5.200.945 5.699 F3.306 5.141.882 5.320 F4.274 5.200.914 5.489 F5.277 5.200.818 5.112 Homogenitas - Daya Sebar Levene Statistic df1 df2 Sig..989 4 20.436 ANOVA - Daya Sebar Sum of Squares df Square F Sig. Between 13057.075 4 3264.269 223.443.000 Within 292.180 20 14.609 Total 13349.254 24 Tukey HSD - Daya Sebar (I) REPLIKASI (J) REPLIKASI 95% Confidence Interval Difference (I-J) Std. Error Sig. Lower Bound Upper Bound F1 F2 21.17000 2.41735.000 13.9364 28.4036 F3 30.23800 2.41735.000 23.0044 37.4716 F4 9.40800 2.41735.007 2.1744 16.6416 F5 66.25600 2.41735.000 59.0224 73.4896 F2 F1-21.17000 2.41735.000-28.4036-13.9364 F3 9.06800 2.41735.010 1.8344 16.3016 F4-11.76200 2.41735.001-18.9956-4.5284 F5 45.08600 2.41735.000 37.8524 52.3196 F3 F1-30.23800 2.41735.000-37.4716-23.0044 F2-9.06800 2.41735.010-16.3016-1.8344 F4-20.83000 2.41735.000-28.0636-13.5964 F5 36.01800 2.41735.000 28.7844 43.2516 F4 F1-9.40800 2.41735.007-16.6416-2.1744 F2 11.76200 2.41735.001 4.5284 18.9956 F3 20.83000 2.41735.000 13.5964 28.0636 F5 56.84800 2.41735.000 49.6144 64.0816 F5 F1-66.25600 2.41735.000-73.4896-59.0224 F2-45.08600 2.41735.000-52.3196-37.8524 F3-36.01800 2.41735.000-43.2516-28.7844 F4-56.84800 2.41735.000-64.0816-49.6144 60
Lampiran 7. Hasil Analisis Viskositas Losion Minyak Atsiri Daun Rosemary Normalitas - Viskositas REPLIKASI Kolmogorov-Smirnova Shapiro-Wilk Statistic df Sig. Statistic df Sig. trans_visk F1.230 5.200.964 5.837 F2.242 5.200.958 5.792 F3.223 5.200.902 5.424 F4.231 5.200.964 5.834 F5.229 5.200.965 5.844 Homogenitas _ Viskositas Levene Statistic df1 df2 Sig. 1.147 4 20.364 ANOVA - Viskositas Sum of Squares df Square F Sig. Between 2.400 4.600 747.323.000 Within.016 20.001 Total 2.417 24 Tukey HSD - Viskositas (I) REPLIKASI (J) REPLIKASI 95% Confidence Interval Difference (I-J) Std. Error Sig. Lower Bound Upper Bound F1 F2 -.12069.01792.000 -.1743 -.0671 F3 -.17783.01792.000 -.2315 -.1242 F4 -.06259.01792.017 -.1162 -.0090 F5 -.85069.01792.000 -.9043 -.7971 F2 F1.12069.01792.000.0671.1743 F3 -.05715.01792.033 -.1108 -.0035 F4.05809.01792.030.0045.1117 F5 -.73001.01792.000 -.7836 -.6764 F3 F1.17783.01792.000.1242.2315 F2.05715.01792.033.0035.1108 F4.11524.01792.000.0616.1689 F5 -.67286.01792.000 -.7265 -.6192 F4 F1.06259.01792.017.0090.1162 F2 -.05809.01792.030 -.1117 -.0045 F3 -.11524.01792.000 -.1689 -.0616 F5 -.78810.01792.000 -.8417 -.7345 F5 F1.85069.01792.000.7971.9043 F2.73001.01792.000.6764.7836 F3.67286.01792.000.6192.7265 F4.78810.01792.000.7345.8417 61
Lampiran 8. Hasil Analisis ph Losion Minyak Atsiri Daun Rosemary Normalitas - ph REPLIKASI Kolmogorov-Smirnova Shapiro-Wilk Statistic df Sig. Statistic df Sig. PH F1.201 5.200.881 5.314 F2.310 5.131.808 5.094 F3.212 5.200.895 5.384 F4.221 5.200.902 5.421 F5.243 5.200.894 5.377 Homogenitas - ph Levene Statistic df1 df2 Sig. 1.939 4 20.143 ANOVA - ph Sum of Squares df Square F Sig. Between 3.755 4.939 62.342.000 Within.301 20.015 Total 4.056 24 Tukey HSD - ph (I) REPLIKASI (J) REPLIKASI 95% Confidence Difference Interval Lower Upper (I-J) Std. Error Sig. Bound Bound F1 F2 -.26400.07760.021 -.4962 -.0318 F3 -.54000.07760.000 -.7722 -.3078 F4.29000.07760.010.0578.5222 F5 -.81000.07760.000-1.0422 -.5778 F2 F1.26400.07760.021.0318.4962 F3 -.27600.07760.015 -.5082 -.0438 F4.55400.07760.000.3218.7862 F5 -.54600.07760.000 -.7782 -.3138 F3 F1.54000.07760.000.3078.7722 F2.27600.07760.015.0438.5082 F4.83000.07760.000.5978 1.0622 F5 -.27000.07760.018 -.5022 -.0378 F4 F1 -.29000.07760.010 -.5222 -.0578 F2 -.55400.07760.000 -.7862 -.3218 F3 -.83000.07760.000-1.0622 -.5978 F5-1.10000.07760.000-1.3322 -.8678 F5 F1.81000.07760.000.5778 1.0422 F2.54600.07760.000.3138.7782 F3.27000.07760.018.0378.5022 F4 1.10000.07760.000.8678 1.3322 62
Lampiran 9. Hasil Analisis Rata-rata Daya Proteksi (%) Atsiri Daun Rosemary Losion Minyak Normalitas - Aktivitas FORMULA Kolmogorov-Smirnova Shapiro-Wilk Statistic df Sig. Statistic df Sig. akt_trans F1.179 6.200.967 6.871 F2.227 6.200.933 6.606 F3.256 6.200.901 6.383 KN.175 6.200.919 6.497 KP.186 6.200.914 6.460 Homogenitas - aktivitas Levene Statistic df1 df2 Sig. 2.524 4 25.066 ANOVA - Aktivitas Sum of Squares df Square F Sig. Between 1.530 4.382 503.273.000 Within.019 25.001 Total 1.549 29 Tukey HSD - Aktivitas (I) FORMULA (J) FORMULA 95% Confidence Interval Difference (I-J) Std. Error Sig. Lower Bound Upper Bound F1 F2.09986.01592.000.0531.1466 F3.17839.01592.000.1316.2251 KN.58107.01592.000.5343.6278 KP -.05936.01592.008 -.1061 -.0126 F2 F1 -.09986.01592.000 -.1466 -.0531 F3.07852.01592.000.0318.1253 KN.48121.01592.000.4345.5279 KP -.15922.01592.000 -.2060 -.1125 F3 F1 -.17839.01592.000 -.2251 -.1316 F2 -.07852.01592.000 -.1253 -.0318 KN.40268.01592.000.3559.4494 KP -.23775.01592.000 -.2845 -.1910 KN F1 -.58107.01592.000 -.6278 -.5343 F2 -.48121.01592.000 -.5279 -.4345 F3 -.40268.01592.000 -.4494 -.3559 KP -.64043.01592.000 -.6872 -.5937 KP F1.05936.01592.008.0126.1061 F2.15922.01592.000.1125.2060 F3.23775.01592.000.1910.2845 KN.64043.01592.000.5937.6872 63
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