LAPORAN RESMI PRAKTIKUM ANALISIS KLINIK PRAKTIKUM IV PENETAPAN KADAR UREA NITROGEN.

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LAPORAN RESMI PRAKTIKUM ANALISIS KLINIK PRAKTIKUM IV PENETAPAN KADAR UREA NITROGEN Hari/ TanggalPercobaan :Jumat / 7 Mei 2010 Golongan/ Kelas : III / FKK 2008 DosenPembimbing : AsistenJaga :Mbak

1 LAPORAN RESMI PRAKTIKUM ANALISIS KLINIK PRAKTIKUM IV PENETAPAN KADAR UREA NITROGEN Hari/ TanggalPercobaan :Jumat / 7 Mei 2010 Golongan/ Kelas : III / FKK 2008 DosenPembimbing : AsistenJaga :Mbak Nurul dan Mbak Indah NamaAnggota : 1. Hivo Grashia Leony FA/ Candra Dwipayana H. FA/ Amelinda Nurjayanti FA/ Fitria Anggraini FA/ Dheny Cahyo Saputro FA/ Teng Thien Wun FA/ Mesa Sukmadani Rusdi FA/ Jasson Yohanes Jr. FA/ Rina Mai Saroh FA/ Dandiko Galanova FA/ Titis Janu Wiyamti FA/ Bakiyarathi Seridaran FA/ Anisa Ika Oktaviani FA/ Hela Putri Kusumo FA/ Hesti Prihastuti D. FA/ Kunthi Hari Pratiwi FA/08165 LABORATORIUM KIMIA FARMASI ANALISIS FAKULTAS FARMASI UNIVERSITAS GADJAH MADA 2010

2 I. TUJUAN Agar mahasiswa mampu menetapkan dan menghitung kadar urea nitrogen dalam sampel darah(serum) dan urin dengan metode enzimatik. (GLDH/ GlutamatDehidrogenase) II. PRINSIP DASAR PERCOBAAN Urea dipecah menjadi ammonia dan karbondioksida dengan pemberian enzim urease. Ammonia yang dibebaskan ditentukan dengan metode urease GLDH enzimatik UV test. Reaksi : H 2 N urease C = O + 2 H 2 O 2 NH CO 2 H 2 N Prinsip reaksi enzimatik urease GLDH : COO - COO - C = O HC NH 2 GLDH CH NH 4 + NADH + H + CH 2 + NAD + +H 2 O CH 2 CH 2 COO - COO - 2-oksoglutarat

3 III. METODE SISTEMATIK Alat dan Bahan Alat : 1. Tabung reaksi 10 ml(3) 2. Beker glass 100 ml (1) 3. Mikropipet (0,5 10 µl dan µl) 4. Yellow tip 5. Blue tip 6. Kuvet 7. Spektrofotometer visible Bahan : 1. Urine probandus 2. Blanko aquadest 3. Reagen 1 Tris ph 7,8 120 mmol/l 2-oksoglutarat 7 mmol/l ADP 0,6 mmol/l Urease 6 ku/l GLDH (Glutamatdehidrogenase) 4. Reagen 2 NADH 1 ku/l 0,25 mmol/l 5. Baku (urea standard FS) 50 mg/dl (8,33 mmol/l) Cara Kerja Sampel urin baku blanko aquadest 10µL 10µL 10µL ± 100 µl Reagen 1

4 Diamkan selama 5 menit ± 250 µl Reagen 2 Diamkan selama 1 menit, baca absorbansi pada λ 340 nm IV. Diamkan selama 1 menit, baca absorbansi pada λ 340 nm DATA DAN PERHITUNGAN a. DATA Sampel Urin: 1 Bagian urin bagian aquadest (pengenceran 101x) Δ Abs Sampel atau baku = (A1-A2) (A blangko 1 -A blanko 2 ) - Kadar urea = Δ Abs Sampel x kons.standar x 101 Δ Abs Baku = x mg/dl - BUN = x.mg/dl x 0,467 = y. mg/dl Kadar Baku : 50 mg/dl Kadar normal = gram/dl

5 Serum Kel Blanko Absorbansi(A) 1 Absorbansi(A) 2 Kadar rata-bun rata-rata rata (mg/dl) Standar Sampel Blanko Standar Sampel (mg/dl) 0,956 0,863 III 0,812 0,668 0,910 0,806 0,645 0,887 1,199 1,192 1,184 3,921 1,831 I 0,892 0,888 0,920 0,912 II IV 1,269 1,247 0,215 V 0, ,817 1,015 4,902 2,289 0,813 0,807 0,945 0,840 32,353 15,109 0,895 0, ,647 8,241 1,245 1,267 0, ,198 0,199

6 Urine Absorbansi(A) 1 Absorbansi(A) 2 Kadar ratarata Kel Blanko Standar Sampel Blanko Standar Sampel (mg/dl) 0,844 0, ,550 I 0,870 0,857 0,763 0,749 0,820 0,806 II 0,795 0,787 0,787 0,782 0,851 0,840 III 0,812 0,668 0,848 0,806 0,645 0,899 0,846 0,836 0,866 0,852 IV 0,834 0,815 0,866 0,796 0,710 0,688 V 0,785 0,714 0,771 0, , , , ,6275

7 B. Perhitungan Serum = Kelompok I Kadar urea Serum 1 = x 50 = Kadar urea Serum 2 = x 50 = = 0 Kadar urea Serum 3 = x 50 = Kadar rata rata = = 3,921 SD = 3,396 CV =86,61 % BUN (Blood Urea Nitrogen) = Kadar X 0,467 BUN I = 5,882 X 0,467 = 2,747 BUN II = 0 X 0,467 = 0 BUN III = 5,882 X 0,467 = 2,747 BUN rata rata = = 1,831 Kelompok II Kadar urea Serum 1 = x 50 = = 0 Kadar urea Serum 2 = x 50 = Kadar urea Serum 3 = x 50 = Kadar rata rata = = 4,902 SD = 8,491 CV =173,22 % BUN (Blood Urea Nitrogen) = Kadar X 0,467 BUN I = 0 X 0,467 = 0 BUN II = 0 X 0,467 = 0 BUN III = 14,706 X 0,467 = 6,868 BUN rata rata = = 2,289

8 Kelompok III Kadar urea Serum 1 = x 50 = Kadar urea Serum 2 = x 50 = Kadar urea Serum 3 = x 50 = Kadar rata rata = = 32,353 SD = 15,563 CV =48,25 % BUN (Blood Urea Nitrogen) = Kadar X 0,467 BUN I = 50 X 0,467 = 23,350 BUN II = 26,471X 0,467 = 12,362 BUN III = 20,588 X 0,467 = 9,615 BUN rata rata = = 15,109 Kelompok IV Kadar urea Serum 1 = x 50 = =0 Kadar urea Serum 2 = x 50 = Kadar urea Serum 3 = x 50 = = 0 Kadar rata rata = = 17,647 SD = 30,566 CV =173,205 % BUN (Blood Urea Nitrogen) = Kadar X 0,467 BUN I = 0 X 0,467 = 0 BUN II = 52,941 X 0,467 = 24,723 BUN III = 0 X 0,467 = 0 BUN rata rata = = 8,241 Kelompok V Kadar urea Serum 1 = x 50 = =0 Kadar urea Serum 2 = x 50 = =0 Kadar urea Serum 3 = x 50 = =0 Kadar rata rata = SD = 0 CV =0 % BUN (Blood Urea Nitrogen) = Kadar X 0,467

9 BUN I = 0 X 0,467 = 0 BUN II = 0 X 0,467 = 0 BUN III = 0 X 0,467 = 0 BUN rata rata = = 0 Urin KELOMPOK 1 Sampel 1 Sampel 2 Sampel 3 Kadar urea rata rata = 2772,550 mg/dl SD = 954,911 CV = 34,442 % KELOMPOK 2 Sampel 1 Sampel 2

10 Sampel 3 Kadar urea rata rata = 990,1367 mg/dl SD = 1260,5239 mg/dl CV = 127,308 % KELOMPOK 3 Sampel 1 Sampel 2 Sampel 3 Kadar urea rata rata = 1188,2353 mg/dl SD = 297,0588 mg/dl CV = 24,9999 % KELOMPOK 4 Sampel 1 Sampel 2

11 Sampel 3 Kadar urea rata rata = 8416,667 mg/dl SD = 9205,629 mg/dl CV = 109,374 % KELOMPOK 5 Sampel 1 Sampel 2 Sampel 3 Kadar urea rata rata = 3268,6275 mg/dl SD = 1372,0559 mg/dl CV = 43,30 % DAFTAR PUSTAKA Richterich, R. and Colombo, J.P., 1981, Clinical Chemistry, Theory, Practice, and Interpretation, John Wiley & Sons, Chichester.

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Tanggal : 05 Maret 2010 LAPORAN PRAKTIKUM ANALISIS KLINIK FKK (SEMESTER GENAP 2010) Golongan : III Nama/NIM : 1. Hivo Grashia Leony FA/08103 2. Candra Dwipayana H FA/08104 3. Amelinda Nurjayanti FA/08123