a. Pemeliharaan hewan coba Mencit yang dipilih adalah mencit yang berumur 2-3 bulan dengan berat rata-rata 20-30 g dan dipelihara di Labaratorium Biokimia Fakultas Kedokteran Universitas Diponegoro. Kandang mencit dipilih yang besar untuk memperluas ruang gerak mencit dan menghindari stress. Mencit diberi makan dan minum yang sama. b. Cara memegang mencit Pengambilan mencit dari kandang pada saat pemberian ekstrak harus pelan-pelan karena mencit akan menggigit apabila ditarik keras-keras. Pengambilan mencit dari kandang dipegang ekornya kemudian diletakkan di kawat kasa dan ekornya ditarik sedikit. Cubit kulit bagian belakang kepala dan jepit ekornya. c. Cara penyuntikan Cubit kulit bagian belakang kepala, kemudian penyuntikan dilakukan pada bagian lateral ekor yang sebelumnya diusap dengan kapas yang dibasahi alkohol. d. Euthanasea Mencit dibunuh dengan melakukan dislokasi pada tulang leher dengan cara menaruh mencit pada permukaan rata, kemudian sebuah benda keras dan tumpul diletakkan di kuduk mencit. Ekor ditarik kuat-kuat sambil menekan benda tersebut di kuduk mencit hingga mati.
Explore Fagositosis makrofag Descriptives Fagositosis makrofag 95% Confidence Interval for Lower Bound Upper Bound Statistic Std. Error.1423.03910.0337.2508 Fagositosis makrofag 2.00 1.50 1.00 0.50 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis 95% Confidence Interval for 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis 95% Confidence Interval for Lower Bound Upper Bound Lower Bound Upper Bound.1441.1667.008.08742.03.22.19.17 -.487.913-2.338 2.000.6876.08027.4813.8939.6892.6799.039.19663.44.91.47.39 -.025.845-2.098 1.741.9526.20139.4349 1.4703 0.00 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis 95% Confidence Interval for Lower Bound Upper Bound.9351.7909.243.49331.50 1.72 1.22.87.780.845 -.957 1.741 1.2510.38429 -.4025 2.9044 Test of Homogeneity of Variances Fagositosis makrofag 5% Trimmed Median Variance Std. Deviation Minimum Maximum Levene Statistic df1 df2 Range Sig. 6.343 3 Interquartile 16 Range.005 Skewness Kurtosis. 1.0256.443.66561.73 2.00 1.27. 1.349 1.225..
Oneway Test of Homogeneity of Variances transform.makrofag Levene Statistic df1 df2 Sig. 2.793 3 16.074 ANOVA transform.makrofag Sum of Squares df Square F Sig. Between Groups 1.367 3.456 11.865.000 Within Groups.615 16.038 Total 1.982 19 Post Hoc Tests Dependent Variable: transform.makrofag Tukey HSD Multiple Comparisons (I) (J) *. The mean difference is significant at the.05 level. Difference 95% Confidence Interval (I-J) Std. Error Sig. Lower Bound Upper Bound -.46386*.11868.006 -.8034 -.1243 -.59164*.11868.001 -.9312 -.2521 -.73521*.14314.001-1.1447 -.3257.46386*.11868.006.1243.8034 -.12777.11316.678 -.4515.1960 -.27135.13859.244 -.6679.1252.59164*.11868.001.2521.9312.12777.11316.678 -.1960.4515 -.14357.13859.731 -.5401.2529.73521*.14314.001.3257 1.1447.27135.13859.244 -.1252.6679.14357.13859.731 -.2529.5401
Homogeneous Subsets Tukey HSD a,b Sig. transform.makrofag Subset for alpha =.05 N 1 2 5.3580 6.8219 6.9497 3 1.0933 1.000.194 s for groups in homogeneous subsets are displayed. a. Uses Harmonic Sample Size = 4.615. b. The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
Produksi Nitric Descriptives Produksi Nitric 95% Confidence Interval for Lower Bound Upper Bound Statistic Std. Error 26.420 6.6134 8.058 44.782 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis 95% Confidence Interval for Lower Bound Upper Bound 27.461 33.000 218.682 14.7879.0 34.1 34.1 17.6-2.222.913 4.950 2.000 36.333.8480 34.153 38.513 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis 95% Confidence Interval for Lower Bound Upper Bound 36.293 36.100 4.315 2.0772 34.3 39.1 4.8 4.1.273.845-2.255 1.741 37.383 1.0489 34.687 40.080 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis 95% Confidence Interval for Lower Bound Upper Bound 37.409 37.500 6.602 2.5694 34.0 40.3 6.3 5.3 -.166.845-1.709 1.741 39.000.5774 36.516 41.484 5% Trimmed Median Variance Std. Deviation Minimum Maximum Range Interquartile Range Skewness Kurtosis. 39.000 1.000 1.0000 38.0 40.0 2.0..000 1.225..
Produksi Nitric Tests of Normality Kolmogorov-Smirnov a *. This is a lower bound of the true significance. a. Lilliefors Significance Correction Shapiro-Wilk Statistic df Sig. Statistic df Sig..447 5.001.600 5.001.240 6.200*.876 6.251.179 6.200*.931 6.585.175 3. 1.000 3 1.000 Test of Homogeneity of Variances Produksi Nitric Levene Statistic df1 df2 Sig. 4.905 3 16.013 50.0 40.0 Produksi Nitric 30.0 20.0 10.0 0.0 1
NPar Tests Kruskal-Wallis Test Ranks Produksi Nitric Total N Rank 5 3.20 6 11.42 6 12.92 3 16.00 20 Test Statistics a,b Chi-Square df Asymp. Sig. Produksi Nitric Oxide (NO) 11.394 3.010 a. Kruskal Wallis Test b. Grouping Variable: NPar Tests Mann-Whitney Test Ranks Produksi Nitric Total N Rank Sum of Ranks 5 3.00 15.00 6 8.50 51.00 11
Mann-Whitney U Wilcoxon W Z Test Statistics b Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. Produksi Nitric Oxide (NO).000 15.000-2.751.006.004 a b. Grouping Variable: NPar Tests Mann-Whitney Test Ranks Produksi Nitric Total N Rank Sum of Ranks 5 3.20 16.00 6 8.33 50.00 11 Mann-Whitney U Wilcoxon W Z Test Statistics b Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. Produksi Nitric Oxide (NO) 1.000 16.000-2.562.010.009 a b. Grouping Variable: NPar Tests Mann-Whitney Test
Produksi Nitric Total Ranks N Rank Sum of Ranks 5 3.00 15.00 3 7.00 21.00 8 Mann-Whitney U Wilcoxon W Z Test Statistics b Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. Produksi Nitric Oxide (NO).000 15.000-2.249.024.036 a b. Grouping Variable: NPar Tests Mann-Whitney Test Ranks Produksi Nitric Total N Rank Sum of Ranks 6 5.92 35.50 6 7.08 42.50 12 Mann-Whitney U Wilcoxon W Z Test Statistics b Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. Produksi Nitric Oxide (NO) 14.500 35.500 -.562.574.589 a b. Grouping Variable:
NPar Tests Mann-Whitney Test Produksi Nitric Total Ranks N Rank Sum of Ranks 6 4.00 24.00 3 7.00 21.00 9 Mann-Whitney U Wilcoxon W Z Test Statistics b Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. Produksi Nitric Oxide (NO) 3.000 24.000-1.556.120.167 a b. Grouping Variable: NPar Tests Mann-Whitney Test Ranks Produksi Nitric Total N Rank Sum of Ranks 6 4.50 27.00 3 6.00 18.00 9
Mann-Whitney U Wilcoxon W Z Test Statistics b Asymp. Sig. (2-tailed) Exact Sig. [2*(1-tailed Sig.)] a. Not corrected for ties. Produksi Nitric Oxide (NO) 6.000 27.000 -.781.435.548 a b. Grouping Variable:
, aquades/hari, pembesaran objektif 40x perlakuan : 80 mg/hari, pembesaran objektif 40x
perlakuan : 400 mg/hari, pembesaran objektif 40x perlakuan : 2000 mg/hari, pembesaran objektif 40x
Mencit yang digunakan dalam penelitian Ekstrak yang telah diencerkan dengan menggunakan aquades Perlakuan dengan cara menyonde
Mencit diinfeksi Salmonella typhimurium Mencit didislokasi cervik Mencit disuntikkan RPMI intraperitoneal
Pengambilan cairan intraperitoneal Kultur makrofag Perhitungan jumlah makrofag