Perhitungan Penetapan Kadar Abu Serbuk Daun Sangitan. W (krus kosong+abu) (gram)

LAMPIRAN A PERHITUNGAN SUSUT PENGERINGAN DAN KADAR ABU SERBUK Perhitungan Susut Pengeringan Serbuk Daun Sangitan Replikasi Hasil susut pengeringan (%b/b) 1,6 2,5 3,6 Rata-rata,6 Replikasi Perhitungan Penetapan Kadar Abu Serbuk Daun Sangitan W serbuk (gram) W (krus kosong+abu) (gram) W (krus kosong) (gram) W abu (gram) Kadar abu (% b/b) 1 2,1430 20,9617 20,25 0,0792 3,70 2 2,1626 19,51 19,4753 0,112 5,22 3 2,132 20,224 20,1922 0,0902 4,23 Rata-rata=4,4 W (krus kosong+abu) W (krus kosong) Kadar abu= 100% W simplisia Contoh perhitungan kadar abu: Kadar abu = W (krus + abu) (g) W krus kosong (g) 100% W simplisia (g) = 20,9617-20,25 100% 2,1430 = 3,70% 6

69 Replikasi LAMPIRAN B PERHITUNGAN KADAR ABU EKSTRAK, KADAR SARI EKSTRAK YANG LARUT DALAM ETANOL Perhitungan Penetapan Kadar Abu Ekstrak Daun Sangitan W ekstrak (gram) W (krus kosong+abu) W (krus kosong) W abu (gram) Kadar abu (% b/b) (gram) (gram) 1 2,1346 19,9302 19,5963 0,3339 15,64 2 2,171 19,1232 1,7541 0,371 17,07 3 2,313 19,077 19,4391 0,3442 14,5 Rata-rata= 16,12 W (krus kosong+abu) W (krus kosong) Kadar abu= 100% W simplisia Contoh perhitungan kadar abu: Kadar abu = W (krus + abu) (g) W krus kosong (g) 100% W simplisia (g) = 19,9302-19,5963 100% = 15,64% 2,1346 69

70 Replikasi Perhitungan Kadar Sari Ekstrak yang Larut dalam Etanol W ekstrak (gram) W (cawan+sari) (gram) W (cawan kosong) (gram) W sari (gram) Kadar sari yang larut dalam etanol (%b/b) 1 5,0191 52,436 52,7219 0,1217 12,12 2 5,0164 52,35 52,7223 0,1162 11,5 3 5,0113 52,572 52,7225 0,1347 13,43 Rata-rata= 12,3 W (cawan+sari) W (cawan kosong) 100 Kadar abu= 100% W ekstrak 20 berat ekstrak kental Randemen ekstrak = 100% berat simplisia 115,071 = 100% 1000 = 11,51 % b/b 70

71 LAMPIRAN C PERHITUNGAN HARGA RF PADA PEMERIKSAAN SECARA KLT Jarak yang ditempuh oleh zat Rf = Jarak yang ditempuh oleh fase gerak Zat Berkhasiat Pengamatan Noda Rf 1 Rf 2 Rf 3 Flavonoid UV 254 nm UV 366 nm Penampak Noda A 3,7 = 0,46 B 3,7 = 0,46 5,0 = 0,62 5,0 = 0,62 C _ 5,0 = A 3,7 = 0,46 B 3,7 = 0,46 0,62 5,0 = 0,62 5,0 = 0,62 C _ 5,0 = 0,62 A _ 5,0 = 0,62 B _ 5,0 = 0,62 C _ 5,0 = Saponin UV 254 nm A 1,2 = 0,62 4,9 = 5,7 = 0,71 5,7 = 0,71 _ 5,7 = 0,71 5,7 = 0,71 7,2 = 0,9 71

72 B 1,2 = C 1,2 = UV 366 nm A 1,2 = Penampak Noda B 1,2 = C 1,2 = A 1,2 = B 1,2 = C 1,2 = 4,9 = 4,9 = 4,9 = 4,9 = 4,9 = 4,9 = 4,9 = 4,9 = 0,1 7,2 = 0,9 _ 7,2 = 0,9 7,2 = 0,9 _ 7,2 = 0,9 7,2 = 0,9 _ 72

73 LAMPIRAN D PERHITUNGAN STATISTIK PERHITUNGAN ANAVA VOLUME TELAPAK KAKI TIKUS PADA JAM KE-0 Descriptives VOLUME N Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 20.0960 5.2542 2.349 13.5720 26.6200 12.56 25.12 5 16.320 5.6170 2.5120 9.3536 23.3024 12.56 25.12 5 1.400.13 3.971 7.125 29.675 12.56 31.40 5 17.540 6.794 3.0766 9.0421 26.1259 12.56 25.12 5 17.540 6.794 3.0766 9.0421 26.1259 12.56 25.12 25 1.064 6.3632 1.2726 15.459 20.7130 12.56 31.40 Test of Homogeneity of Variances VOLUME Levene Statistic df1 df2 Sig. 1.937 4 20.143 ANOVA VOLUME Between Groups Within Groups Sum of Squares df Square F Sig. 41.016 4 10.254.220.924 930.746 20 46.537 971.762 24 73

74 Post Hoc Test Multiple Comparisons Dependent Variable: VOLUME Tukey HSD (I) FORMULA (J) FORMULA Difference Interval (I-J) Std. Error Sig. Bound Bound 3.760 4.3145.903-9.1427 16.677 1.2560 4.3145.99-11.6547 14.1667 2.5120 4.3145.976-10.397 15.4227 2.5120 4.3145.976-10.397 15.4227-3.760 4.3145.903-16.677 9.1427-2.5120 4.3145.976-15.4227 10.397-1.2560 4.3145.99-14.1667 11.6547-1.2560 4.3145.99-14.1667 11.6547-1.2560 4.3145.99-14.1667 11.6547 2.5120 4.3145.976-10.397 15.4227 1.2560 4.3145.99-11.6547 14.1667 1.2560 4.3145.99-11.6547 14.1667-2.5120 4.3145.976-15.4227 10.397 1.2560 4.3145.99-11.6547 14.1667-1.2560 4.3145.99-14.1667 11.6547.0000 4.3145 1.000-12.9107 12.9107-2.5120 4.3145.976-15.4227 10.397 1.2560 4.3145.99-11.6547 14.1667-1.2560 4.3145.99-14.1667 11.6547.0000 4.3145 1.000-12.9107 12.9107 74

75 LAMPIRAN E PERHITUNGAN ANAVA VOLUME TELAPAK KAKI TIKUS PADA JAM KE-2 Descriptives VOLUME N Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 2.0 5.6170 2.5120 21.9136 35.624 1.4 31.40 5 2.0 5.6170 2.5120 21.9136 35.624 25.12 37.6 5 31.4000.13 3.971 20.3725 42.4275 25.12 43.96 5 2.0 5.6170 2.5120 21.9136 35.624 25.12 37.6 5 30.1440 6.794 3.0766 21.6021 3.659 25.12 37.6 25 29.6416 6.1531 1.2306 27.1017 32.115 1.4 43.96 Test of Homogeneity of Variances VOLUME Levene Statistic df1 df2 Sig. 1.51 4 20.21 ANOVA VOLUME Between Groups Within Groups Sum of Squares df Square F Sig. 25.241 4 6.310.143.964 3.420 20 44.171 90.661 24 75

76 Post Hoc Tests Multiple Comparisons Dependent Variable: VOLUME Tukey HSD (I) FORMULA (J) FORMULA Difference Interval Lower Upper (I-J) Std. Error Sig. Bound Bound.0000 4.2034 1.000-12.572 12.572-2.5120 4.2034.974-15.0902 10.0662.0000 4.2034 1.000-12.572 12.572-1.2560 4.2034.99-13.342 11.3222.0000 4.2034 1.000-12.572 12.572-2.5120 4.2034.974-15.0902 10.0662.0000 4.2034 1.000-12.572 12.572-1.2560 4.2034.99-13.342 11.3222 2.5120 4.2034.974-10.0662 15.0902 2.5120 4.2034.974-10.0662 15.0902 2.5120 4.2034.974-10.0662 15.0902 1.2560 4.2034.99-11.3222 13.342.0000 4.2034 1.000-12.572 12.572.0000 4.2034 1.000-12.572 12.572-2.5120 4.2034.974-15.0902 10.0662-1.2560 4.2034.99-13.342 11.3222 1.2560 4.2034.99-11.3222 13.342 1.2560 4.2034.99-11.3222 13.342-1.2560 4.2034.99-13.342 11.3222 1.2560 4.2034.99-11.3222 13.342 76

77 LAMPIRAN F PERHITUNGAN ANAVA VOLUME TELAPAK KAKI TIKUS PADA JAM KE-4 Descriptives VOLUME N Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 32.6560 5.2542 2.349 26.1320 39.100 25.12 37.6 5 23.640 5.2542 2.349 17.3400 30.30 1.4 31.40 5 27.6320 7.1603 3.2022 1.7413 36.5227 1.4 37.6 5 26.3760 5.2542 2.349 19.520 32.9000 1.4 31.40 5 27.6320 9.5241 4.2593 15.063 39.4577 1.4 37.6 25 27.6320 6.732 1.3566 24.320 30.4320 1.4 37.6 Test of Homogeneity of Variances VOLUME Levene Statistic df1 df2 Sig. 1.700 4 20.19 ANOVA VOLUME Between Groups Within Groups Sum of Squares df Square F Sig. 205.00 4 51.270 1.140.366 99.196 20 44.960 1104.275 24 77

7 Post Hoc Tests Multiple Comparisons Dependent Variable: VOLUME Tukey HSD (I) FORMULA (J) FORMULA Difference Interval (I-J) Std. Error Sig. Bound Bound.7920 4.2407.270-3.90 21.420 5.0240 4.2407.760-7.6660 17.7140 6.200 4.2407.56-6.4100 1.9700 5.0240 4.2407.760-7.6660 17.7140 -.7920 4.2407.270-21.420 3.90-3.760 4.2407.9-16.450.9220-2.5120 4.2407.975-15.2020 10.170-3.760 4.2407.9-16.450.9220-5.0240 4.2407.760-17.7140 7.6660 3.760 4.2407.9 -.9220 16.450 1.2560 4.2407.99-11.4340 13.9460.0000 4.2407 1.000-12.6900 12.6900-6.200 4.2407.56-1.9700 6.4100 2.5120 4.2407.975-10.170 15.2020-1.2560 4.2407.99-13.9460 11.4340-1.2560 4.2407.99-13.9460 11.4340-5.0240 4.2407.760-17.7140 7.6660 3.760 4.2407.9 -.9220 16.450.0000 4.2407 1.000-12.6900 12.6900 1.2560 4.2407.99-11.4340 13.9460 7

79 LAMPIRAN G PERHITUNGAN ANAVA VOLUME TELAPAK KAKI TIKUS PADA JAM KE-6 Descriptives VOLUME N Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 35.160 5.6170 2.5120 2.1936 42.1424 25.12 37.6 5 21.3520 3.4397 1.533 17.011 25.6229 1.4 25.12 5 26.3760.11 3.661 16.2091 36.5429 1.4 37.6 5 23.640 6.794 3.0766 15.3221 32.4059 1.4 31.40 5 25.1200 6.200 2.05 17.3224 32.9176 1.4 31.40 25 26.3760 7.4747 1.4949 23.2906 29.4614 1.4 37.6 Test of Homogeneity of Variances VOLUME Levene Statistic df1 df2 Sig. 1.464 4 20.250 ANOVA VOLUME Between Groups Within Groups Sum of Squares df Square F Sig. 552.13 4 13.034 3.500.025 7.76 20 39.43 1340.906 24 79

0 Post Hoc Tests Multiple Comparisons Dependent Variable: VOLUME Tukey HSD (I) FORMULA (J) FORMULA *. The mean difference is significant at the.05 level. Interval Difference (I-J) Std. Error Sig. Bound Bound 13.160* 3.971.01 1.9307 25.7013.7920 3.971.215-3.0933 20.6773 11.3040 3.971.067 -.513 23.193 10.040 3.971.123-1.373 21.9333-13.160* 3.971.01-25.7013-1.9307-5.0240 3.971.715-16.9093 6.613-2.5120 3.971.96-14.3973 9.3733-3.760 3.971.74-15.6533.1173 -.7920 3.971.215-20.6773 3.0933 5.0240 3.971.715-6.613 16.9093 2.5120 3.971.96-9.3733 14.3973 1.2560 3.971.99-10.6293 13.1413-11.3040 3.971.067-23.193.513 2.5120 3.971.96-9.3733 14.3973-2.5120 3.971.96-14.3973 9.3733-1.2560 3.971.99-13.1413 10.6293-10.040 3.971.123-21.9333 1.373 3.760 3.971.74 -.1173 15.6533-1.2560 3.971.99-13.1413 10.6293 1.2560 3.971.99-10.6293 13.1413 0

1 LAMPIRAN H PERHITUNGAN ANAVA VOLUME TELAPAK KAKI TIKUS PADA JAM KE- Descriptives VOLUME N Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 3.9360 5.2542 2.349 32.4120 45.4600 31.40 43.96 5 16.320 5.6170 2.5120 9.3536 23.3024 12.56 25.12 5 22.600.4255 3.760 12.1464 33.0696 12.56 31.40 5 1.400 6.200 2.05 11.0424 26.6376 12.56 25.12 5 1.400 6.200 2.05 11.0424 26.6376 12.56 25.12 25 23.1104 10.2102 2.0420 1.95 27.3250 12.56 43.96 Test of Homogeneity of Variances VOLUME Levene Statistic df1 df2 Sig..56 4 20.507 ANOVA VOLUME Between Groups Within Groups Sum of Squares df Square F Sig. 1665.7 4 416.470 9.962.000 36.094 20 41.05 2501.972 24 1

2 Post Hoc Tests Multiple Comparisons Dependent Variable: VOLUME Tukey HSD (I) FORMULA (J) FORMULA Difference *. The mean difference is significant at the.05 level. Interval (I-J) Std. Error Sig. Bound Bound 22.600* 4.092.000 10.3714 34.446 16.320* 4.092.006 4.0914 2.5646 20.0960* 4.092.001 7.594 32.3326 20.0960* 4.092.001 7.594 32.3326-22.600* 4.092.000-34.446-10.3714-6.200 4.092.553-1.5166 5.9566-2.5120 4.092.971-14.746 9.7246-2.5120 4.092.971-14.746 9.7246-16.320* 4.092.006-2.5646-4.0914 6.200 4.092.553-5.9566 1.5166 3.760 4.092.5 -.466 16.0046 3.760 4.092.5 -.466 16.0046-20.0960* 4.092.001-32.3326-7.594 2.5120 4.092.971-9.7246 14.746-3.760 4.092.5-16.0046.466.0000 4.092 1.000-12.2366 12.2366-20.0960* 4.092.001-32.3326-7.594 2.5120 4.092.971-9.7246 14.746-3.760 4.092.5-16.0046.466.0000 4.092 1.000-12.2366 12.2366 2

3 LAMPIRAN I PERHITUNGAN ANAVA LEUKOSIT TIKUS PADA JAM KE-0 LEUCOSIT N Descriptives Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 7100.00 30.79 170.29 6627.19 7572.1 6650 7600 5 7510.00 926.96 414.55 6359.03 660.97 6400 550 5 7210.00 765.1 342.20 6259.90 160.10 6400 050 5 720.00 1001.62 447.94 6036.32 523.6 5900 400 5 720.00 49.39 21.6 7212.35 427.65 7150 450 25 734.00 735.09 147.02 700.57 767.43 5900 550 Test of Homogeneity of Variances LEUCOSIT Levene Statistic df1 df2 Sig. 2.439 4 20.01 ANOVA LEUCOSIT Between Groups Within Groups Sum of Squares df Square F Sig. 163600 4 409650.0.723.56 1.1E+07 20 566500.0 1.3E+07 24 3

4 Post Hoc Tests Multiple Comparisons Dependent Variable: LEUCOSIT Tukey HSD (I) FORMULA (J) FORMULA Difference Interval (I-J) Std. Error Sig. Bound Bound -410.00 476.03.90-134.46 1014.46-110.00 476.03.999-1534.46 1314.46-10.00 476.03.995-1604.46 1244.46-720.00 476.03.567-2144.46 704.46 410.00 476.03.90-1014.46 134.46 300.00 476.03.96-1124.46 1724.46 230.00 476.03.9-1194.46 1654.46-310.00 476.03.964-1734.46 1114.46 110.00 476.03.999-1314.46 1534.46-300.00 476.03.96-1724.46 1124.46-70.00 476.03 1.000-1494.46 1354.46-610.00 476.03.705-2034.46 14.46 10.00 476.03.995-1244.46 1604.46-230.00 476.03.9-1654.46 1194.46 70.00 476.03 1.000-1354.46 1494.46-540.00 476.03.77-1964.46 4.46 720.00 476.03.567-704.46 2144.46 310.00 476.03.964-1114.46 1734.46 610.00 476.03.705-14.46 2034.46 540.00 476.03.77-4.46 1964.46 4

5 LAMPIRAN J PERHITUNGAN ANAVA LEUKOSIT TIKUS PADA JAM KE-2 LEUCOSIT N Descriptives Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 12900.00 711.51 31.20 12016.54 1373.46 12050 13700 5 13340.00 66.39 29.91 12510.0 14169.92 12700 14400 5 1330.00 659.17 294.79 12561.54 1419.46 12650 14450 5 12460.00 1061.4 474.71 11141.99 1377.01 11400 1350 5 13010.00 536.66 240.00 12343.65 13676.35 12200 13550 25 1301.00 764.02 152.0 12702.63 13333.37 11400 14450 Test of Homogeneity of Variances LEUCOSIT Levene Statistic df1 df2 Sig. 1.462 4 20.251 ANOVA LEUCOSIT Between Groups Within Groups Sum of Squares df Square F Sig. 200400 4 700100.0 1.249.322 1.1E+07 20 560450.0 1.4E+07 24 5

6 Post Hoc Tests Multiple Comparisons Dependent Variable: LEUCOSIT Tukey HSD (I) FORMULA (J) FORMULA Difference Interval (I-J) Std. Error Sig. Bound Bound -440.00 473.4.2-156.3 976.3-40.00 473.4.46-196.3 936.3 440.00 473.4.2-976.3 156.3-110.00 473.4.999-1526.3 1306.3 440.00 473.4.2-976.3 156.3-40.00 473.4 1.000-1456.3 1376.3 0.00 473.4.370-536.3 2296.3 330.00 473.4.955-106.3 1746.3 40.00 473.4.46-936.3 196.3 40.00 473.4 1.000-1376.3 1456.3 920.00 473.4.32-496.3 2336.3 370.00 473.4.933-1046.3 176.3-440.00 473.4.2-156.3 976.3-0.00 473.4.370-2296.3 536.3-920.00 473.4.32-2336.3 496.3-550.00 473.4.772-1966.3 66.3 110.00 473.4.999-1306.3 1526.3-330.00 473.4.955-1746.3 106.3-370.00 473.4.933-176.3 1046.3 550.00 473.4.772-66.3 1966.3 6

7 LAMPIRAN K PERHITUNGAN ANAVA LEUKOSIT TIKUS PADA JAM KE-4 LEUCOSIT N Descriptives Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 14630.00 24.17 127.0 14277.16 1492.4 14350 15050 5 1110.00 65.02 306.35 10959.44 12660.56 1000 12500 5 1110.00 469.4 210.12 10596.62 11763.3 10650 1100 5 10740.00 954.6 427.02 9554.39 11925.61 900 12200 5 1170.00 623.10 27.66 11006.32 12553.6 1000 12350 25 1202.00 1507.90 301.5 11405.57 12650.43 900 15050 Test of Homogeneity of Variances LEUCOSIT Levene Statistic df1 df2 Sig. 1.992 4 20.135 ANOVA LEUCOSIT Between Groups Within Groups Sum of Squares df Square F Sig. 4.6E+07 4 1.2E+07 27.941.000 23000 20 414150.0 5.5E+07 24 7

Post Hoc Tests Multiple Comparisons Dependent Variable: LEUCOSIT Tukey HSD (I) FORMULA (J) FORMULA Difference *. The mean difference is significant at the.05 level. Interval (I-J) Std. Error Sig. Bound Bound 220.00* 407.01.000 1602.05 4037.95 3450.00* 407.01.000 2232.05 4667.95 390.00* 407.01.000 2672.05 5107.95 250.00* 407.01.000 1632.05 4067.95-220.00* 407.01.000-4037.95-1602.05 630.00 407.01.545-57.95 147.95 1070.00 407.01.103-147.95 227.95 30.00 407.01 1.000-117.95 1247.95-3450.00* 407.01.000-4667.95-2232.05-630.00 407.01.545-147.95 57.95 440.00 407.01.14-777.95 1657.95-600.00 407.01.590-117.95 617.95-390.00* 407.01.000-5107.95-2672.05-1070.00 407.01.103-227.95 147.95-440.00 407.01.14-1657.95 777.95-1040.00 407.01.11-2257.95 177.95-250.00* 407.01.000-4067.95-1632.05-30.00 407.01 1.000-1247.95 117.95 600.00 407.01.590-617.95 117.95 1040.00 407.01.11-177.95 2257.95

9 LAMPIRAN L PERHITUNGAN ANAVA LEUKOSIT TIKUS PADA JAM KE-6 LEUCOSIT N Descriptives Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 16010.00 604.57 270.37 15259.33 16760.67 15300 1600 5 9660.00 70.54 349.07 690.3 10629.17 650 10550 5 970.00 407.12 12.07 9364.49 10375.51 9400 10450 5 9500.00 116.91 530.0 026.26 10973.74 400 11450 5 10340.00 1320.23 590.42 700.72 11979.2 9400 12650 25 11076.00 2671.73 534.35 9973.16 1217.4 400 1600 Test of Homogeneity of Variances LEUCOSIT Levene Statistic df1 df2 Sig. 1.169 4 20.354 ANOVA LEUCOSIT Between Groups Within Groups Sum of Squares df Square F Sig. 1.5E+0 4 3.9E+07 44.91.000 1.7E+07 20 5450.0 1.7E+0 24 9

90 Post Hoc Tests Multiple Comparisons Dependent Variable: LEUCOSIT Tukey HSD (I) FORMULA (J) FORMULA Difference *. The mean difference is significant at the.05 level. Interval (I-J) Std. Error Sig. Bound Bound 6350.00* 55.99.000 4596.49 103.51 6140.00* 55.99.000 436.49 793.51 6510.00* 55.99.000 4756.49 263.51 5670.00* 55.99.000 3916.49 7423.51-6350.00* 55.99.000-103.51-4596.49-210.00 55.99.996-1963.51 1543.51 160.00 55.99.999-1593.51 1913.51-60.00 55.99.773-2433.51 1073.51-6140.00* 55.99.000-793.51-436.49 210.00 55.99.996-1543.51 1963.51 370.00 55.99.96-133.51 2123.51-470.00 55.99.927-2223.51 123.51-6510.00* 55.99.000-263.51-4756.49-160.00 55.99.999-1913.51 1593.51-370.00 55.99.96-2123.51 133.51-40.00 55.99.614-2593.51 913.51-5670.00* 55.99.000-7423.51-3916.49 60.00 55.99.773-1073.51 2433.51 470.00 55.99.927-123.51 2223.51 40.00 55.99.614-913.51 2593.51 90

91 LAMPIRAN M PERHITUNGAN ANAVA LEUKOSIT TIKUS PADA JAM KE- LEUCOSIT N Descriptives Interval for Std. Deviation Std. Error Bound Bound Minimum Maximum 5 17410.00 969.79 433.70 16205.4 1614.16 16100 1700 5 310.00 3.46 395.09 7213.04 9406.96 7100 9400 5 220.00 65.20 306.43 7369.21 9070.79 7350 9050 5 7990.00 90.51 39.25 64.29 9095.71 6600 9050 5 590.00 406.2 11.93 04.7 9095.13 100 9200 25 10104.00 303.12 760.62 534.15 11673.5 6600 1700 Test of Homogeneity of Variances LEUCOSIT Levene Statistic df1 df2 Sig..717 4 20.590 ANOVA LEUCOSIT Between Groups Within Groups Sum of Squares df Square F Sig. 3.3E+0 4.4E+07 132.794.000 1.3E+07 20 62900.0 3.5E+0 24 91

92 Post Hoc Tests Multiple Comparisons Dependent Variable: LEUCOSIT Tukey HSD (I) FORMULA (J) FORMULA Difference *. The mean difference is significant at the.05 level. Interval (I-J) Std. Error Sig. Bound Bound 9100.00* 501.92.000 759.06 10601.94 9190.00* 501.92.000 76.06 10691.94 9420.00* 501.92.000 791.06 10921.94 20.00* 501.92.000 731.06 10321.94-9100.00* 501.92.000-10601.94-759.06 90.00 501.92 1.000-1411.94 1591.94 320.00 501.92.967-111.94 121.94-20.00 501.92.90-171.94 1221.94-9190.00* 501.92.000-10691.94-76.06-90.00 501.92 1.000-1591.94 1411.94 230.00 501.92.990-1271.94 1731.94-370.00 501.92.945-171.94 1131.94-9420.00* 501.92.000-10921.94-791.06-320.00 501.92.967-121.94 111.94-230.00 501.92.990-1731.94 1271.94-600.00 501.92.754-2101.94 901.94-20.00* 501.92.000-10321.94-731.06 20.00 501.92.90-1221.94 171.94 370.00 501.92.945-1131.94 171.94 600.00 501.92.754-901.94 2101.94 92

93 LAMPIRAN N PERHITUNGAN KONVERSI VOLUME KAKI TIKUS Hasil Pengukuran Volume Telapak Kaki Tikus Putih yang diberi Larutan PGA 3% b/v Per Oral Tikus No Volume Telapak Kaki Tikus (ml) pada Jam Ke- 0 2 4 6 1 0,2 0,25 0,3 0,3 0,35 2 0,2 0,25 0,3 0,3 0,35 3 0,25 0,25 0,3 0,3 4 0,25 0,25 0,3 0,3 5 0,1 0,2 0,2 0,25 0,0 1,15 1,30 1,40 1,55 Rata-rata 0,16 0,23 0,26 0,2 0,31 Hasil Pengukuran Volume Telapak Kaki Tikus Putih yang diberi Suspensi Fenilbutazone 9 mg/kgbb (0,09% b/v) Per Oral Tikus No Volume Telapak Kaki Tikus (ml) pada Jam Ke- 0 2 4 6 1 0,2 0,3 0,25 0,2 0,2 2 0,1 0,2 0,2 0,1 3 0,1 0,2 0,1 4 0,25 0,2 0,2 5 0,1 0,2 0,1 0,65 1,15 0,95 0,5 0,65 Rata-rata 0,13 0,23 0,19 0,17 0,13 93

94 Tikus No Hasil Pengukuran Volume Telapak Kaki Tikus Putih yang diberi SuspensiEkstrak Daun Sangitan 1,0g/kgBB (10% b/v) Per Oral Volume Telapak Kaki Tikus (ml) pada Jam Ke- 0 2 4 6 1 0,2 0,3 0,25 0,25 0,25 2 0,25 0,35 0,3 0,3 0,25 3 0,1 0,2 0,2 0,2 4 0,1 0,2 0,2 5 0,1 0,2 0,1 0,75 1,25 1,1 1,05 0,9 Rata-rata 0,25 0,22 0,21 0,1 Hasil Pengukuran Volume Telapak Kaki Tikus Putih yang diberi Suspensi Ekstrak Daun Sangitan 1,5 g/kgbb (15% b/v) Per Oral Tikus No Volume Telapak Kaki Tikus (ml) pada Jam Ke- 0 2 4 6 1 0,2 0,25 0,25 0,25 0,2 2 0,2 0,3 0,25 0,25 0,2 3 0,1 0,2 0,1 4 0,1 0,2 0,2 5 0,1 0,2 0,2 0,1 0,7 1,15 1,05 0,95 0,75 Rata-rata 0,14 0,23 0,21 0,19 94

95 Hasil Pengukuran Volume Telapak Kaki Tikus Putih yang diberi Suspensi Ekstrak Daun Sangitan 2,0 g/kgbb (20% b/v) Per Oral Tikus No Volume Telapak Kaki Tikus (ml) pada Jam Ke- 0 2 4 6 1 0,2 0,3 0,3 0,25 0,2 2 0,2 0,3 0,3 0,25 0,2 3 0,1 0,2 0,1 4 0,1 0,2 0,2 0,1 5 0,1 0,2 0,2 0,7 1,2 1,1 1,0 0,75 Rata-rata 0,14 0,24 0,22 0,2 Volume kaki tikus = 3,14 x r 2 x Vol kaki (dlm mm) Keterangan= r: jari-jari pada tabung Phletysmometer (diketahui = 2mm), vol kaki (dlm mm): vol kaki pada pengamatan diubah dalam mm Contoh perhitungan: Volume kaki tikus PGA 3% pd jam ke-0, tikus 1 = 3,14 x 2 2 x 2 =25,12 mm 3 Perhitungan % Radang Vol pd t n-1 Vol pd t n % Radang = x 100% Vol pd t n Contoh perhitungan : 2,9-20,10 % Radang tikus PGA 3% = x 100% 20,10 = 43,75 95

96 Perhitungan % Inhibisi Vol (E/P) pd t n -t 0 -- Vol (k) pd t n -t 0 % inhibisi = x 100% Vol (k) pd t n -t 0 Keterangan = Vol (E/P) pd t n -t 0 : Volume telapak kaki tikus ekstrak atau tikus pembanding (mm 3 ) pada jam yang akan dihitung dikurangi pada jam ke-0, Vol (k) pd t n -t 0 : Volume telapak kaki tikus kontrol pada jam yang akan dihitung dikurangi pada jam ke-0. 96

97 LAMPIRAN O 97

9 LAMPIRAN P TABEL KOEFISIEN KORELASI r S Sumber: Snedeene (1946) 9

99 LAMPIRAN Q SURAT DETERMINASI TUMBUHAN SANGITAN 99