Lampiran. 1 Metode Pembuatan Ekstrak Air Jahe Rimpang Jahe Serbuk Dibersihkan Dicuci Dikering anginkan Ditimbang Dihaluskan dan diayak Ditimbang Dimasukan kedalam botol kaca bersih Dimaserasi ± 48 jam dengan pelarut air yang telah didestilasi Maserat Disaring Filtrat Residu Diwaterbath Ekstrak kental Fresh dryer Ekstrak kering Direndam dengan pelarut air yang telah didestilasi ± 1 hari dan diaduk Disaring Filtrat Residu
Lampiran 2. Tabel 6. Data Kadar MDA dalam Testis Mencit Jantan Dewasa (µm/ml) Ulangan 1 9,031 13,849 15,439 12,045 6,482 6,929 2 12,442 11,614 11,366 16,896 9,429 8,733 3 9,230 15,306 8,949 10,488 6,978 16,167 4 6,515 14,570 17,690 9,611 7,409 9,131 x ±SD 9,305±2,429 13,835±1,595 13,361±3,937 12,260±3,250 7,575±1,293 10,240±4,066 Uji Normalitas MDA Testis, Uji Homogenitas MDA Testis, dan Oneway ANOVA MDA_testis Kelompok a. Lilliefors Significance Correction Hasil Uji: Data berdistribusi normal (p>0,05) Uji Homogenitas MDA Testis MDA_testis Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Tests of Normality Kolmogorov-Smirnov a Hasil Uji: Variansi Data Homogen normal (p>0,05) Shapiro-Wilk Statistic df Sig. Statistic df Sig..262 4..952 4.731.254 4..925 4.563.201 4..961 4.785.276 4..875 4.317.301 4..877 4.325.357 4..830 4.167 Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. 1.569 5 18.219.818 5 18.552.818 5 9.718.564 1.427 5 18.262
Lanjutan Lampiran 2. Oneway ANOVA MDA_testis Sum of Squares df Mean Square F Sig. Between Groups 121.316 5 24.263 2.762.051 Within Groups 158.152 18 8.786 Total 279.468 23 Hasil Uji: Tidak terdapat perbedaan yang nyata antar perlakuan p=0,051 (p>0,05)
Lampiran 3. Data Diameter Tubulus Seminiferus Testis. Tabel 7. Data Diameter Tubulus Seminiferus Testis. Ulangan 1 135,33 169,75 184,47 176,78 178,45 111,61 2 148,43 216,36 136,14 170,45 195,61 123,82 3 172,93 137,15 171,38 162,2 121,34 114,63 4 147,95 171,99 156,33 168,23 120,86 68,66 x ±SD 151,16±15,73 173,81±32,53 162,08±20,77169,42±6,02154,07±38,70104,68±24,57 Diameter_TS Kelompok Tests of Normality Tebal_TS Hasil Uji: Data berdistribusi normal (p>0,05) Diameter_TS Based on Mean Based on Median Based on Median and with adjusted df Kolmogorov-Smirnov a Statistic df Sig. Statistic df Sig..319 4..905 4.457 Based on trimmed mean Hasil Tebal_TS Uji: Variansi Based Data on Mean Homogen normal (p>0,05) Shapiro-Wilk.272 4..946 4.694.173 4..986 4.937.182 4..992 4.969.301 4..825 4.154.361 4..815 4.132.286 4..937 4.636 Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. 2.618 5 18.060 1.870 5 18.150 1.870 5 9.981.187 2.510 5 18.068
Lanjutan Lampiran 3. Spread vs. Level Plot of Tebal_TS by Kelompok 3.6 3.3 Spread 3.0 2.7 2.4 2.1 3.60 3.65 3.70 3.75 Level 3.80 3.85 3.90 * Plot of LN of Spread vs LN of Level Slope = 2.907 Power for transformation = -1.907 Uji Normalitas dan Uji Homogenitas Diameter Tubulus Seminiferus Testis setelah data ditransformasi trans_tebal_ts Kelompok a. Lilliefors Significance Correction Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig..252 4..957 4.762.435 4..639 4.002.243 4..939 4.649.247 4..892 4.395.256 4..916 4.516.305 4..752 4.040 Test of Homogeneity of Variance trans_tebal_ts Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Levene Statistic df1 df2 Sig. 3.412 5 18.024.677 5 18.646.677 5 4.329.663 2.795 5 18.049
Lanjutan Lampiran 3. Oneway Diameter_TS Between Groups Within Groups Total Hasil: Tebal Sel Epitel Tubulus Seminiferus berbeda nyata antara masing-masing perlakuan. Jadi perlu dilakukan analisis lanjut dengan Analisis Post Hoc - Bonferroni. Post Hoc Tests ANOVA Sum of Squares df Mean Square F Sig. 12492.424 5 2498.485 3.869.015 11623.976 18 645.776 24116.400 23
Lanjutan Lampiran 3. Multiple Comparisons Dependent Variable: Diameter_TS Bonferroni (I) Kelompok (J) Kelompok *. The mean difference is significant at the.05 level. Mean Difference 95% Confidence Interval (I-J) Std. Error Sig. Lower Bound Upper Bound -22.65250 17.96909 1.000-83.3945 38.0895-10.92000 17.96909 1.000-71.6620 49.8220-18.25500 17.96909 1.000-78.9970 42.4870-2.90500 17.96909 1.000-63.6470 57.8370 46.48000 17.96909.279-14.2620 107.2220 22.65250 17.96909 1.000-38.0895 83.3945 11.73250 17.96909 1.000-49.0095 72.4745 4.39750 17.96909 1.000-56.3445 65.1395 19.74750 17.96909 1.000-40.9945 80.4895 69.13250* 17.96909.018 8.3905 129.8745 10.92000 17.96909 1.000-49.8220 71.6620-11.73250 17.96909 1.000-72.4745 49.0095-7.33500 17.96909 1.000-68.0770 53.4070 8.01500 17.96909 1.000-52.7270 68.7570 57.40000 17.96909.075-3.3420 118.1420 18.25500 17.96909 1.000-42.4870 78.9970-4.39750 17.96909 1.000-65.1395 56.3445 7.33500 17.96909 1.000-53.4070 68.0770 15.35000 17.96909 1.000-45.3920 76.0920 64.73500* 17.96909.031 3.9930 125.4770 2.90500 17.96909 1.000-57.8370 63.6470-19.74750 17.96909 1.000-80.4895 40.9945-8.01500 17.96909 1.000-68.7570 52.7270-15.35000 17.96909 1.000-76.0920 45.3920 49.38500 17.96909.198-11.3570 110.1270-46.48000 17.96909.279-107.2220 14.2620-69.13250* 17.96909.018-129.8745-8.3905-57.40000 17.96909.075-118.1420 3.3420-64.73500* 17.96909.031-125.4770-3.9930-49.38500 17.96909.198-110.1270 11.3570
Lampiran 4. Data Tebal Epitel Tubulus Seminiferus Testis. Tabel 8. Data Tebal Lapisan Epitel Tubulus Seminiferus Testis. Ulangan 1 38,54 46,69 47,97 21,53 60,60 57,62 2 38,95 17,97 35,56 31,30 69,97 60,21 3 45,64 47,67 41,87 44,07 31,49 30,85 4 34,36 47,65 44,53 48,05 38,16 30,93 Rata2 39,37±4,66 40,00±14,69 42,48±5,25 36,24±12,13 50,06±18,20 44,90±16,21 Uji Normalitas dan Uji Homogenitas Tebal Spermatogenik dalam tubulus seminiferus. Diameter_TS Tebal_TS Kelompok a. Lilliefors Significance Correction Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig..286 4..937 4.636.426 4..654 4.003.204 4..974 4.868.241 4..938 4.641.243 4..916 4.516.306 4..768 4.056 Test of Homogeneity of Variance Diameter_TS Tebal_TS Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Levene Statistic 2.510 df1 5 df2 18 Sig..068 5.474 5 18.003 2.306 5 18.087 2.306 5 5.082.188 4.897 5 18.005 NPar Tests Kruskal-Wallis Test Ranks Tebal_TS Kelompok Total N Mean Rank 4 10.75 4 13.00 4 13.25 4 10.00 4 15.50 4 12.50 24
Lanjutan Lampiran 4. Test Statistics a,b Chi-Square df Asymp. Sig. Tebal_TS 1.530 5.910 a. Kruskal Wallis Test b. Grouping Variable: Kelompok Hasil: Tebal Sel Epitel Tubulus Seminiferus berbeda tidak nyata antara masingmasing perlakuan. Jadi tidak perlu dilakukan analisis lanjut.
Lampiran 5. Konversi perhitungan dosis untuk berbagai jenis hewan dan manusia
Lampiran 6. Surat Rekomendasi Persetujuan Etik Penelitian Kesehatan
Lampiran 7. Hasil Identifikasi Tumbuhan
Lampiran 8. Hasil Skrining Fitokimia Senyawa Bahan Alam