Lampiran 1. Data Penukuran ph Sediaan Perlakuan Formula I II III IV 1 7,9 7,9 7,9 7,9 7,9 7,9 7,9 7,9 3 7,9 7,9 7,9 7,9 Rata-rata 7,9 7,9 7,9 7,9
Lampiran. Data Penentuan Viskositas Sediaan Hara Viskositas Masin-Masin Formula Perlakuan Formula I (P) II (P) III (P) IV (P) 1 66,9011 76,8053 138,5718 8, 4775 70,107 79,6749 139,1108 9,7477 3 67,8977 78,6349 140,0540 30,400 Rata-rata 68,3065 78,3717 139,455 9,544 Keteranan : P satuan Poise Perhitunan Viskositas Masin-Masin Formula Spesifikasi Formula I II III IV Berat (ram) 6,506 5,73 5,816 5,846 Volume (ml) 10 10 10 10 Waktu (detik) t 1 3491 3988 7199 1187 t 3659 4137 77 11938 t 3 3543 4083 776 1197 Perhitunan Penentuan Viskositas Formula: K Keteranan : ( ρk f ) η K ρ r 9l t η K ρk ρf t r : viskositas (Poise) : Konstanta viskosimeter : Berat jenis bola (/ml) 16,31 /ml : Berat jenis sediaan (krim /ml) : Waktu (detik) : Jari-jari bola (m) 7,5 x 10-3 m
l : Panjan antara tanda (m) 0,1 m : Percepatan rafitasi bumi (9,88 m/s ) K 3 ( 7,5 10 ) 9,88 3 9 0,1 1,3 10 Formula I ρ f massa volume 6,506 10 ml 0,6506 η 1,3 10 ml ml ( 16,31 0,6506) 3491 3 1 66,9011 Poise 3 η 1,3 10 ml 70,107 Poise ( 16,31 0,6506) 3659 3 η3 1,3 10 ml 67,8977 Poise ( 16,31 0,6506) 3543 detik detik detik η η 1 + η + η3 ( 66,9011+ 70,107 + 67,8977) Poise 68,3065 Poise
Formula II ρ f massa volume 5,73 10 ml 0,573 ml 3 η1 1,3 10 ml 76,8053 Poise ( 16,31 0,573) 3988 3 η 1,3 10 ml 79,6749 Posie ( 16,31 0,573) 4137 3 η3 1,3 10 ml 78,6349 Poise ( 16,31 0,573) 4083 detik detik detik η η 1 + η + η3 ( 76,8053 + 79,6749 + 78,6349) Poise 78,3717 Poise Formula III ρ f massa volume 5,816 10 ml 0,5816 ml
3 η1 1,3 10 ml 138,5718 Poise ( 16,31 0,5816) 7199 detik 3 η 1,3 10 (16,31 0,5816) 77 detik ml 139,1108 Poise 3 η3 1,3 10 (16,31 0,5816) 776 detik ml 140,0540 Poise η η 1 + η + η3 ( 138,5718 + 139,1108 + 140,0540) Poise 139,455 Poise Formula IV ρ f massa volume 5,846 10 ml 0,5846 ml 3 η1 1,3 10 (16,31 0,5846) 1187 detik ml 8,4775 Poise 3 η 1,3 10 (16,31 0,5846) 11938detik ml 9,7477 Poise 3 η3 1,3 10 (16,31 0,5846) 1197 detik ml 30,400 Poise
η η 1 + η + η3 ( 8,4775 + 9,7477 + 30,400) Poise 9,544 Poise
Lampiran 3. Data Penentuan Tipe Emulsi Sediaan No. Formula Tipe Emulsi 1 I m/a II m/a 3 III m/a 4 IV m/a Keteranan : Formula I : Formula tabir surya tanpa penambahan zink oksida Formula II : Formula tabir surya denan penambahan zink oksida 5% Formula III : Formula tabir surya denan penambahan zink oksida 10% Formula IV : Formula tabir surya denan penambahan zink oksida 15% m/a : minyak dalam air
Lampiran 4. Data Penukuran Serapan Masin-masin Formula Data Penukuran Serapan Formula I Penulanan Serapan Pada Panjan Gelomban (λ) (nm) 90 300 310 30 330 340 350 360 1 0.3181 0.3186 0.359 0.798 0.1758 0.0808 0.0433 0.0189 0.350 0.350 0.3344 0.864 0.179 0.084 0.0465 0.0178 3 0.336 0.339 0.353 0.3055 0.197 0.099 0.0530 0.08 4 0.3314 0.3317 0.3488 0.3011 0.1895 0.0906 0.0544 0.075 5 0.99 0.3013 0.3113 0.660 0.1604 0.0691 0.0463 0.0160 6 0.319 0.3107 0.345 0.789 0.1741 0.0798 0.0570 0.033 Rata-rata 0.3199 0.300 0.339 0.863 0.1786 0.089 0.0501 0.011 Data Penukuran Serapan Formula II Penulanan Serapan Pada Panjan Gelomban (λ) (nm) 90 300 310 30 330 340 350 360 1 0.3719 0.379 0.3833 0.33 0.115 0.1067 0.065 0.0367 0.3441 0.3480 0.3585 0.3098 0.1987 0.1017 0.0646 0.0356 3 0.3497 0.3501 0.3716 0.337 0.101 0.1089 0.0695 0.0398 4 0.3689 0.368 0.3884 0.3373 0.169 0.1118 0.075 0.0447 5 0.3538 0.3580 0.376 0.3184 0.1978 0.093 0.0636 0.033 6 0.3578 0.3574 0.3748 0.33 0.036 0.0990 0.0709 0.0358 Rata-rata 0.3577 0.3591 0.3749 0.340 0.064 0.1034 0.0673 0.0375 Data Penukuran Serapan Formula III Penulanan Serapan Pada Panjan Gelomban (λ) (nm) 90 300 310 30 330 340 350 360 1 0.3966 0.398 0.4070 0.3535 0.38 0.157 0.0746 0.0509 0.3690 0.3713 0.3804 0.390 0.130 0.1104 0.0657 0.0380 3 0.3979 0.3976 0.419 0.3674 0.43 0.1334 0.0837 0.0559 4 0.383 0.3804 0.3979 0.3480 0.30 0.156 0.0798 0.056 5 0.3755 0.3783 0.3904 0.3376 0.166 0.108 0.0745 0.0446 6 0.3804 0.3794 0.393 0.3413 0.03 0.113 0.0785 0.0437 Rata-rata 0.3838 0.384 0.3980 0.3461 0.60 0.1193 0.0761 0.048
Data Penukuran Serapan Formula IV Penulanan Serapan Pada Panjan Gelomban (λ) (nm) 90 300 310 30 330 340 350 360 1 0.4366 0.4373 0.4431 0.3883 0.604 0.1466 0.08 0.0656 0.4014 0.4034 0.4084 0.3561 0.356 0.190 0.0713 0.05 3 0.446 0.4469 0.4650 0.4096 0.767 0.1589 0.0948 0.0741 4 0.4059 0.4070 0.4196 0.3690 0.493 0.1436 0.0884 0.0697 5 0.4044 0.4083 0.4159 0.3613 0.35 0.145 0.0780 0.0554 6 0.407 0.408 0.4143 0.3590 0.31 0.100 0.078 0.0460 Rata-rata 0.416 0.4176 0.477 0.3739 0.48 0.1371 0.0813 0.0605
Lampiran 5. Contoh Perhitunan Nilai SPF Panjan elomban (λ) ( nm) A 90 0.3181 300 0.3186 310 0.359 30 0.798 330 0.1758 340 0.0808 350 0.0433 AUC L L L L L L serapanλ + serapanλ n n+ 1 ( )( λ λ ) n+ 1 0.3181+ 0.3186 ( ) X ( 300 90) 3. 1835 1 0.3186+ 0.359 ( ) X ( 310 300) 3. 5 0.359+ 0.798 ( ) X ( 30 310) 3. 085 3 0.798+ 0.1758 ( ) X ( 330 30). 780 4 0.1758+ 0.0808 ( ) X ( 340 330) 1. 830 5 0.0808+ 0.0433 ( ) X ( 350 340) 0, 605 6 AUC L + 1 + L + L3 + L4 + L5 L6 3.1835 + 3.5 + 3.085 +.780 + 1.830 + 0.605 13.616 n AUC Lo SPF λ maks λ min 13.616 350 90 0.4538
SPF Arc. Lo SPF Arc 0.4538.8435
Lampiran 6. Grafik Serapan Sediaan Tabir Surya Gambar a. Grafik Serapan Sediaan Tabir Surya Pada Penukuran 1 Gambar b. Grafik Serapan Sediaan Tabir Surya Pada Penukuran
Gambar c. Grafik Serapan Sediaan Tabir Surya Pada Penukuran 3 Gambar d. Grafik Serapan Sediaan Tabir Surya Pada Penukuran 4
Gambar e. Grafik Serapan Sediaan Tabir Surya Pada Penukuran 5 Gambar f. Grafik Sediaan Tabir Surya Pada Penukuran 6
Lampiran 7. Data Uji Iritasi Terhadap Kulit Sukarelawan No. Reaksi Pada Kulit Sukarelawan 1 3 4 5 6 1 Iritasi - - - - - - Gatal - - - - - - 3 Kulit menjadi kasar - - - - - -
Lampiran 8. Analis Zat Aktif
Lampiran 9. Gambar Sediaan Tabir Surya Gambar 3. Sediaan Formula Tabir Surya
Lampiran 10. Gambar Uji Homoenitas Sediaan Tabir Surya Gambar 4a. Uji Homoenitas Formula I (Formula tabir surya tanpa penambahan ZnO) Gambar 4b. Uji Homoenitas Formula II (Formula tabir surya denan penambahan ZnO 5%) Gambar 4c. Uji Homoenitas Formula III (Formula tabir surya denan penambahan ZnO 10%)
Gambar 4d. Uji Homoenitas Formula IV (Formula tabir surya denan penambahan ZnO 15%)
Lampiran 11. Penamatan Stabilitas Sediaan Metode Sentrifuasi Gambar 5a. Penamatan pada jam pertama Gambar 5b. Penamatan pada jam kedua Gambar 5c. Penamatan pada jam ketia
Gambar 5d. Penamatan pada jam keempat Gambar 5e. Penamatan pada jam kelima
Lampiran 1. Penentuan Tipe Emulsi Sediaan Tabir Surya Gambar 6a. Tipe emulsi m/a sediaan tabir surya formula I Gambar 6b. Tipe emulsi m/a sediaan tabir surya formula II Gambar 6c. Tipe emulsi m/a sediaan tabir surya formula III
Gambar 6d. Tipe emulsi m/a sediaan tabir surya formula IV
Lampiran 13. Gambar Alat yan Diunakan Gambar 7. Neraca Analitis Baeco Gambar 8. Viskometer Bola Jatuh Hoppler
Gambar 9. Mikroskop Gambar 10. Alat Sentrifus H110T Centrifue Gambar 11. Spektrofotometer UV-VIS mini 140 (Shimadzu)
Lampiran 14. Perhitunan Statitik Metode ANOVA Rancan Acak Lenkap Ulanan (r) Perlakuan (t) I II III IV 1 I1 II1 III1 IV1 I II III IV 3 I3 II3 III3 IV3 4 I4 II4 III4 IV4 5 I5 II5 III5 IV5 6 I6 II6 III6 IV6 Jumlah (TA) ΣI ΣII ΣIII ΣIV Rataan (ya) y I y II y III y IV Sumber Variasi Df SS MS F Hitun F tabel Perlakuan t-1 SST MST Error Percobaan n-t SSE MSE Total SSy MST MSE F Tabel {F(0,05);(t-1);(n-t)} Keteranan : SS T Treament of Squares atau Jumlah Kuadrat Perlakuan SS E Error of Squares atau Jumlah Kuadrat Error SS y Jumlah Kuadrat Total Df Deree of Fredom atau Tinkat Kebebasan t Perlakuan n Jumlah Subjek dalam Percobaan MS Means Squares MS T SST t 1 MS E SSE n t
Data Perhitunan ANOVA No. Sun Protectin Factor / SPF F1 F F3 F4 1.8435 3.4770 3.8394 4.4160.9173 3.138 3.471 3.8870 3 3.0881 3.3470 3.986 4.7409 4 3.0539 3.599 3.736 4.0915 5.676 3.804 3.5647 3.9397 6.847 3.3405 3.6195 3.8783 Jumlah 17.4001 0.1886.046 4.9534 Rata-rata.9000 3.3648 3.7008 4.1589 SST t 1 t X r i Y tr ( 17,4001 + 0,1886 +,046 + 4,9534 ) (,8435 +,9173+ 3,0881+ 3,0539 + + 3,8783) 6 186,0699 718,0031 6 4 304,3449 99,501 5,0947 4 6 t r X i Y SSY 1 t j 1 r tr (,8435 +,9173 + 3,0881 + + 3,8783 ) 305,39 6,078 99,501 4 (,8435 +,9173+ 3,0881+ + 3,8783) 4 6 SSE SSY SST 6,078 5,0947 0,9781
SST MST t 1 5,0947 4 1 1,698 MSE SSE n t F hitun 0,9781 4 4 0,0489 MST MSE 1,698 0,0489 34,780 F Tabel {F(0,05);(t-1);(n-t)} F(0,05);(4-1);(4-4) F(0,05);(3);(0) 3,10 F hitun > F tabel dilakukan uji lanjutan
Perhitunan Nilai HSD HSD qα (, n t) t MSE r 0,0489 q0,05(4,0) 6 3,96 0,0903 0,35758
Lampiran 15. Hasil Perhitunan ANOVA Descriptives SPF N Mean 95% Confidence Interval Std. for Mean Std. Error Deviation Lower Upper Minimum Maximum Bound Bound 1 6,900017,1548801,06395,737480 3,06553,676 3,0881 6 3,364767,1188937,0485381 3,39995 3,489538 3,138 3,599 3 6 3,700767,18958,077511 3,50186 3,899347 3,471 3,986 4 6 4,158900,3493084,146046 3,7933 4,55477 3,8783 4,7409 Total 4 3,531113,5138451,104888 3,314135 3,748090,676 4,7409 Test of Homoeneity of Variances SPF Levene Statistic df1 df Si. 3,441 3 0,036 SPF ANOVA Sum of Squares Df Mean Square F Si. Between Groups 5,093 3 1,698 34,657,000 Within Groups,980 0,049 Total 6,073 3
Multiple Comparisons Dependent Variable: SPF Tukey HSD (I) formula (J) formula Mean Difference (I-J) Std. Error Si. 95% Confidence Interval Upper Bound Lower Bound 1 -,4647500(*),177844,008 -,8410 -,107090 3 -,8007500(*),177844,000-1,158410 -,443090 4-1,588833(*),177844,000-1,616544 -,9013 1,4647500(*),177844,008,107090,8410 3 -,3360000,177844,070 -,693660,01660 4 -,7941333(*),177844,000-1,151794 -,436473 3 1,8007500(*),177844,000,443090 1,158410,3360000,177844,070 -,01660,693660 4 -,4581333(*),177844,009 -,815794 -,100473 4 1 1,588833(*),177844,000,9013 1,616544,7941333(*),177844,000,436473 1,151794 3,4581333(*),177844,009,100473,815794 * The mean difference is sinificant at the.05 level. Tukey HSD SPF Formula N Subset for alpha.05 1 3 1 6,900,017 6 3,364,767 3 6 3,700,767 4 6 4,158,900 Si. 1,000,070 1,000 Means for roups in homoeneous subsets are displayed. a Uses Harmonic Mean Sample Size 6,000.
Lampiran 16. Tabel q df Number of treatments 3 4 5 6 7 8 9 10 11 1 13 14 15 16 17 18 19 0 1 18.0 7.0 3.8 37. 40.5 43.1 45.4 47.3 49.1 50.6 51.9 53. 54.3 55.4 56.3 57. 58.0 58.8 59.6 6.09 8.33 9.80 10.89 11.73 1.43 13.03 13.54 13.99 14.39 14.75 15.08 15.38 15.65 15.91 16.14 16.36 16.57 16.77 3 4.50 5.91 6.83 7.51 8.04 8.47 8.85 9.18 9.46 9.7 9.95 10.16 10.35 10.5 10.69 10.84 10.98 11.1 1.4 4 3.93 5.04 5.76 6.9 6.71 7.06 7.35 7.60 7.83 8.03 8.1 8.37 8.5 8.67 8.80 8.9 9.03 9.14 9.4 5 3.64 4.60 5. 5.67 6.03 6.33 6.58 6.80 6.99 7.17 7.3 7.47 7.60 7.7 7.83 7.93 8.03 8.1 8.1 6 3.46 4.34 4.90 5.31 5.63 5.89 6.1 6.3 6.49 6.65 6.79 6.9 7.04 7.14 7.4 7.34 7.43 7.51 7.59 7 3.34 4.16 4.68 5.06 5.35 5.59 5.80 5.99 6.15 6.9 6.4 6.54 6.65 6.75 6.84 6.93 7.01 7.08 7.16 8 3.6 4.04 4.53 4.89 5.17 5.40 5.60 5.77 5.9 6.05 6.18 6.9 6.39 6.48 6.57 6.65 6.73 6.80 6.87 9 3.0 3.95 4.4 4.76 5.0 5.4 5.43 5.60 5.74 5.87 5.98 6.09 6.19 6.8 6.36 6.44 6.51 6.58 6.65 10 3.15 3.88 4.33 4.66 4.91 5.1 5.30 5.46 5.60 5.7 5.83 5.93 6.03 6.1 6.0 6.7 6.34 6.41 6.47 11 3.11 3.8 4.6 5.58 4.8 5.03 5.0 5.35 5.49 5.61 5.71 5.81 5.90 5.98 6.06 6.14 6.0 6.7 6.33 1 3.08 3.77 4.0 4.51 4.75 4.95 5.1 5.7 5.40 5.51 5.61 5.71 5.80 5.88 5.95 6.0 6.09 6.15 6.1 13 3.06 3.73 4.15 4.46 4.69 4.88 5.05 5.19 5.3 5.43 5.53 5.63 5.71 5.79 5.86 5.93 6.00 6.06 6.11 14 3.03 3.70 4.11 4.41 4.64 4.83 4.99 5.13 5.5 5.36 5.46 5.56 5.64 5.7 5.79 5.86 5.9 5.98 6.03 15 3.01 3.67 4.08 4.37 4.59 4.78 4.94 5.08 5.0 5.31 5.40 5.49 5.57 5.65 5.7 5.79 5.85 5.91 5.96 16 3.00 3.65 4.05 4.34 4.56 4.74 4.90 5.03 5.15 5.6 5.35 5.44 5.5 5.59 5.66 5.73 5.79 5.84 5.90 17.98 3.6 4.0 4.31 4.5 4.70 4.86 4.99 5.11 5.1 5.31 5.39 5.47 5.55 5.61 5.68 5.74 5.79 5.84 18.97 3.61 4.00 4.8 4.49 4.67 4.83 4.96 5.07 5.17 5.7 5.35 5.43 5.50 5.57 5.63 5.69 5.74 5.79 19.96 3.59 3.98 4.6 4.47 4.64 4.79 4.9 5.04 5.14 5.3 5.3 5.39 5.46 5.53 5.59 5.65 5.70 5.75 0.95 3.58 3.96 4.4 4.45 4.6 4.77 4.90 5.01 5.11 5.0 5.8 5.36 5.43 5.50 5.56 5.61 5.66 5.71 30.89 3.48 3.84 4.11 4.30 4.46 4.60 4.7 4.83 4.9 5.00 5.08 5.15 5.1 5.7 5.33 5.38 3.43 5.48 40.86 3.44 3.79 4.04 4.3 4.39 4.5 4.63 4.74 4.8 4.90 4.98 5.05 5.11 5.17 5. 5.7 5.3 5.36 10.80 3.6 3.69 3.9 4.10 4.4 4.36 4.47 4.56 4.64 4.71 4.78 4.84 4.90 4.95 5.00 5.04 5.09 5.13.77 3.3 3.63 3.86 4.03 4.17 4.9 4.39 4.47 4.55 4.6 4.68 4.74 4.80 4.84 4.89 4.93 4.97 5.01