= 0,04 ml/ekor. = 0,001 mg/g BB

LAMPIRAN

57 LAMPIRAN 1 PERHITUNGAN DOSIS 1. Perhitungan dosis pemberian Anthelmentik Albendazole 5% = 5 g/100ml = 50 gram/ml Dosis untuk mencit = 10 mg/kg BB Dosis untuk mencit BB 20 gram = = = 0,04 ml/ekor 2. Perhitungan dosis pemberian Antibiotik (Clavamox ) Konsentrasi = 125 mg/ml = 25 mg/ml Dosis untuk anak-anak BB 25 kg = 1 mg/kg BB/hari Dosis untuk mencit = = 0,001 mg/g BB Dosis untuk mencit BB 20 gram = = 0,0008 ml/ekor/hari 3. Perhitungan dosis pemberian Antiprotozoa (Flagyl ) Sediaan 500 mg/tablet Dosis untuk manusia BB 50 kg = 1500 mg/50 kg BB/hari = 30 mg/kg BB/hari Dosis untuk mencit = = 0,03 mg/g BB Dosis untuk mencit BB 25 gram = 25 (gram) x 0,03 mg/g BB = 0,75 mg/ekor/hari

58 4. Perhitungan Dosis Pemakaian Jintan Hitam Dosis Pemakaian = 1-2 sdm 3x sehari Dosis Preventif = = 0,3 ml/kg x 2 sdm = 0,6 ml/kg = x 0,6 x 3 = 0,054 ml 0,1 ml/ hari Dosis kuratif = 2x Dosis Preventif = 0,2 ml/ hari Dosis Hs madu = 3x Dosis Preventif = 0,3 ml/ hari

59 LAMPIRAN 2 JADWAL Jadwal Pelaksanaan Penelitian Waktu Kegiatan Juni 2010 Mempersiapkan dan membersihkan Fasilitas Kandang Hewan Percobaan Bagian Patologi FKH IPB Persiapan peralatan perkandangan hewan coba seperti box kandang, alas kain, tempat makan, dan tempat minum 16 Agustus 2010 Mencit untuk hewan percobaan dimasukkan ke dalam kandang dan diistirahatkan selama 2 hari agar dapat beradaptasi dengan kandang 20 Agustus 2010 Pemberian antihelmintik pada mencit 23-27 Agustus 2010 Pemberian antibiotik pada mencit 28 Agustus 2010 Pemberian antihelmintik pada mencit 30 Agustus-3 September 2010 Pemberian antijamur pada mencit 16 September-16 November 2010 Pemberian ekstrak minyak jintan hitam dan kombinasi madu pada kelompok perlakuan 22 November 2010 Nekropsi mencit 29 November 2010 Trimming organ mencit 1-15 Desember 2010 Pembuatan preparat histopatologi Januari -Februari 2011 Pengamatan preparat histopatologi

60 LAMPIRAN 3 HASIL ANALISIS DATA LIMFONODUS JANTAN Jumlah * Perlakuan Case Processing Summary Cases Included Excluded Total N Percent N Percent N Percent 11 100.0% 0.0% 11 100.0% Luas * Perlakuan 11 100.0% 0.0% 11 100.0% Jumlah * Sampel 11 100.0% 0.0% 11 100.0% Luas * Sampel 11 100.0% 0.0% 11 100.0% Jumlah Luas * Perlakuan Perlakuan Jumlah Luas Kontrol Mean 6.5000 71.5000 N 2 2 Std. Deviation.70711 1.484921 Pre Mean 6.6667 1.2167E2 Std. Deviation 2.88675 1.258311 Ku Mean 4.0000 2.01002 Std. Deviation.00000 3.119291 Madu Mean 4.3333 3.06672 Std. Deviation 1.52753 6.806861 Total Mean 5.2727 1.84642 N 11 11 Std. Deviation 1.95402 9.732551

61 Sum of ANOVA Squares df Mean Square F Sig. Sampel Between.409 3.136.147.928 Within 6.500 7.929 Total 6.909 10 Jumlah Between 16.348 3 5.449 1.747.244 Within 21.833 7 3.119 Total 38.182 10 Luas Between 82972.712 3 27657.571 16.477.001 Within 11749.833 7 1678.548 Total 94722.545 10 Duncan Jumlah Perlakuan N Subset for alpha = 0.05 1 Kuraftif 3 4.0000 Madu 3 4.3333 Kontrol 2 6.5000 Preventif 3 6.6667 Sig..143 Means for groups in homogeneous subsets are displayed.

62 Luas Duncan Subset for alpha = 0.05 Perlakuan N 1 2 3 Kontrol 2 71.5000 Preventif 3 1.2167 1.2167 Kuratif 3 2.0100 Madu 3 3.0667 Sig..200.060 1.000 Means for groups in homogeneous subsets are displayed.

63 LAMPIRAN 4 HASIL ANALISIS DATA LIMFONODUS BETINA Jumlah * Perlakuan Case Processing Summary Cases Included Excluded Total N Percent N Percent N Percent 11 100.0% 0.0% 11 100.0% Luas * Perlakuan 11 100.0% 0.0% 11 100.0% Jumlah * Sampel 11 100.0% 0.0% 11 100.0% Luas * Sampel 11 100.0% 0.0% 11 100.0% Jumlah Luas * Perlakuan Perlakuan Jumlah Luas Kontrol Mean 5.5000 78.0000 N 2 2 Std. Deviation.70711 1.13137 Pre Mean 6.6667 1.61002 Std. Deviation 1.52753 6.82862 Ku Mean 6.0000 2.51332 Std. Deviation 2.64575 8.25671 Madu Mean 6.6667 3.42672 Std. Deviation 3.51188 1.02632 Total Mean 6.2727 2.20092 N 11 11 Std. Deviation 2.14900 1.105252

64 Sampel Between Jumlah Between Luas ANOVA Sum of Squares df Mean Square F Sig..409 3.136.147.928 Within 6.500 7.929 Total 6.909 10 2.348 3.783.125.942 Within 43.833 7 6.262 Total 46.182 10 Between 98857.576 3 32952.525 9.900.007 Within 23299.333 7 3328.476 Total 122156.909 10 Duncan Jumlah Perlakuan N Subset for alpha = 0.05 1 Kontrol 2 5.5000 Ku 3 6.0000 Pre 3 6.6667 Madu 3 6.6667 Sig..624 Means for groups in homogeneous subsets are displayed.

65 Luas Duncan Subset for alpha = 0.05 Perlakuan N 1 2 3 Kontrol 2 78.0000 Pre 3 1.6100 1.6100 Ku 3 2.5133 2.5133 Madu 3 3.4267 Sig..141.114.110 Means for groups in homogeneous subsets are displayed.

66 LAMPIRAN 5 HASIL ANALISIS DATA LIMPA JANTAN Jumlah * Perlakuan Case Processing Summary Cases Included Excluded Total N Percent N Percent N Percent 11 100.0% 0.0% 11 100.0% Luas * Perlakuan 11 100.0% 0.0% 11 100.0% Jumlah * Sampel 11 100.0% 0.0% 11 100.0% Luas * Sampel 11 100.0% 0.0% 11 100.0% Jumlah Luas * Perlakuan Perlakuan Jumlah Luas Kontrol Mean 6.5000 1.4250 N 2 2 Std. Deviation 2.12132 4.59619 Pre Mean 7.3333 3.2500 Std. Deviation 1.15470 4.68615 Ku Mean 6.0000 4.8767 Std. Deviation 2.64575 7.40968 Madu Mean 6.3333 5.2333 Std. Deviation 1.52753 1.33088 Total Mean 6.5455 3.9027 N 11 11 Std. Deviation 1.69491 1.64331

67 Sampel Between Jumlah Between Luas ANOVA Sum of Squares df Mean Square F Sig..409 3.136.147.928 Within 6.500 7.929 Total 6.909 10 2.894 3.965.261.851 Within 25.833 7 3.690 Total 28.727 10 Between 217136.348 3 72378.783 9.576.007 Within 52909.833 7 7558.548 Total 270046.182 10 Duncan Jumlah Perlakuan N Subset for alpha = 0.05 1 Ku 3 6.0000 Madu 3 6.3333 Kontrol 2 6.5000 Pre 3 7.3333 Sig..471 Means for groups in homogeneous subsets are displayed.

68 Luas Duncan Subset for alpha = 0.05 Perlakuan N 1 2 3 Kontrol 2 1.4250 Pre 3 3.2500 Ku 3 4.8767 4.8767 Madu 3 5.2333 Sig. 1.000.068.650 Means for groups in homogeneous subsets are displayed.

69 LAMPIRAN 6 HASIL ANALISIS DATA LIMPA BETINA Jumlah * Perlakuan Case Processing Summary Cases Included Excluded Total N Percent N Percent N Percent 11 100.0% 0.0% 11 100.0% Luas * Perlakuan 11 100.0% 0.0% 11 100.0% Jumlah * Sampel 11 100.0% 0.0% 11 100.0% Luas * Sampel 11 100.0% 0.0% 11 100.0% Jumlah Luas * Perlakuan Perlakuan Jumlah Luas Kontrol Mean 8.0000 1.1500 N 2 2 Std. Deviation.00000 7.07107 Pre Mean 6.3333 2.4533 Std. Deviation 2.08167 7.25282 Ku Mean 6.0000 4.6833 Std. Deviation 1.00000 1.97791 Madu Mean 6.0000 5.6767 Std. Deviation 3.46410 1.67112 Total Mean 6.4545 3.7036 N 11 11 Std. Deviation 2.01810 2.16232

70 Sampel Between Jumlah Between Luas ANOVA Sum of Squares df Mean Square F Sig..409 3.136.147.928 Within 6.500 7.929 Total 6.909 10 6.061 3 2.020.408.752 Within 34.667 7 4.952 Total 40.727 10 Between 322898.545 3 107632.848 5.208.033 Within 144666.000 7 20666.571 Total 467564.545 10 Duncan Jumlah Perlakuan N Subset for alpha = 0.05 1 Ku 3 6.0000 Madu 3 6.0000 Pre 3 6.3333 Kontrol 2 8.0000 Sig..357 Means for groups in homogeneous subsets are displayed.

71 Luas Duncan Subset for alpha = 0.05 Perlakuan N 1 2 3 Kontrol 2 1.1500 Pre 3 2.4533 2.4533 Ku 3 4.6833 4.6833 Madu 3 5.6767 Sig..330.116.451 Means for groups in homogeneous subsets are displayed.