LAMPIRAN A. Data dan Analisis Statistik Berat Hepar Mencit Rataan Berat Hepar Mencit (Mus musculus L.) dengan Pemberian Vitamin C dan E setelah Pemajanan Monosodium Glutamat (MSG) (g, X±SD) Kelompok Perlakuan X±SD K- 1.62 ±0.09 K+ 1.97 ±0.26 P1 1.85 ±0.19 P2 2.12 ±0.30 P3 2.22 ±0.08 P4 2.13 ±0.30 Hasil Uji Statistik Berat Hepar Mencit Tests of Normality Kel_Per lakuan Kolmogorov-Smirnov a Shapiro-Wilk Statistic Df Sig. Statistic df Sig. Berat_Hepar K-.190 5.200 *.949 5.730 K+.300 5.161.776 5.051 P1.159 5.200 *.979 5.929 P2.240 5.200 *.860 5.230 P3.215 5.200 *.931 5.603 P4.227 5.200 *.905 5.440 a. Lilliefors Significance Correction *. This is a lower bound of the true significance. Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. Berat_Hepar Based on Mean 2.327 5 24.074 Based on Median.953 5 24.466 Based on Median and with adjusted df.953 5 14.450.477 Based on trimmed mean 2.183 5 24.090
Oneway ANOVA Berat_Hepar Sum of Squares Df Mean Square F Sig. Between Groups 1.220 5.244 4.975.003 Within Groups 1.177 24.049 Total 2.397 29 Post-Hoc Multiple Comparisons Berat_Hepar Bonferroni (I) (J) Mean Difference 95% Confidence Interval Kel_Perlakuan Kel_Perlakuan (I-J) Std. Error Sig. Lower Bound Upper Bound K- K+ -.34600.14007.315 -.8024.1104 P1 -.22800.14007 1.000 -.6844.2284 P2 -.49800 *.14007.024 -.9544 -.0416 P3 -.60000 *.14007.004-1.0564 -.1436 P4 -.50200 *.14007.022 -.9584 -.0456 K+ K-.34600.14007.315 -.1104.8024 P1.11800.14007 1.000 -.3384.5744 P2 -.15200.14007 1.000 -.6084.3044 P3 -.25400.14007 1.000 -.7104.2024 P4 -.15600.14007 1.000 -.6124.3004 P1 K-.22800.14007 1.000 -.2284.6844 K+ -.11800.14007 1.000 -.5744.3384 P2 -.27000.14007.987 -.7264.1864 P3 -.37200.14007.208 -.8284.0844 P4 -.27400.14007.933 -.7304.1824 P2 K-.49800 *.14007.024.0416.9544 K+.15200.14007 1.000 -.3044.6084 P1.27000.14007.987 -.1864.7264 P3 -.10200.14007 1.000 -.5584.3544 P4 -.00400.14007 1.000 -.4604.4524
P3 K-.60000 *.14007.004.1436 1.0564 K+.25400.14007 1.000 -.2024.7104 P1.37200.14007.208 -.0844.8284 P2.10200.14007 1.000 -.3544.5584 P4.09800.14007 1.000 -.3584.5544 P4 K-.50200 *.14007.022.0456.9584 K+.15600.14007 1.000 -.3004.6124 P1.27400.14007.933 -.1824.7304 P2.00400.14007 1.000 -.4524.4604 P3 -.09800.14007 1.000 -.5544.3584 *. The mean difference is significant at the 0.05 level.
LAMPIRAN B. Data dan Analisis Statistik Kerusakan Histologis Hepar Mencit Rataan Masing-masing Derajat Kerusakan (Degenerasi Parenkimatosa, Degenerasi Hidropik, dan Nekrosis) Histologis Hepar Mencit (Mus musculus L.) dengan Pemberian Vitamin C dan E setelah Pemajanan Monosodium Glutamat (MSG) (X±SD) Kelompok Perlakuan Degenerasi Parenkimatosa X±SD Degenerasi Hidropik X±SD Nekrosis X±SD K- 61.2±6.10 45±12.55 20±4.90 K+ 54±10.49 33±13.25 18±8.49 P1 58±9.49 127.2±20.74 44±29.26 P2 49.2±9.65 83.4±23.75 52.8±38.72 P3 67.6±7.13 52.2±18.32 18.4±16.15 P4 57.2±8.32 48±22.65 11.2±7.69 Hasil Uji Statistik Histologis Hepar Kruskal-Wallis Test Kel_Perlakuan N Mean Rank K- 5 14.40 K+ 5 9.20 P1 5 23.90 P2 5 19.30 P3 5 14.40 P4 5 11.80 Total 30 Test Statistics a,b Chi-Square 12.415 df 5 Asymp. Sig..030 a. Kruskal Wallis Test
Mann-Whitney Test K-K+ Kel_Perlakuan N Mean Rank Sum of K- 5 6.50 32.50 K+ 5 4.50 22.50 Mann-Whitney U 7.500 Wilcoxon W 22.500 Z -1.225 Asymp. Sig. (2-tailed).221.310 a K-P1 Kel_Perlakuan N Mean Rank Sum of K- 5 3.80 19.00 P1 5 7.20 36.00 Mann-Whitney U 4.000 Wilcoxon W 19.000 Z -2.032 Asymp. Sig. (2-tailed).042.095 a
K-P2 Kel_Perlakuan N Mean Rank Sum of K- 5 4.60 23.00 P2 5 6.40 32.00 Mann-Whitney U 8.000 Wilcoxon W 23.000 Z -1.342 Asymp. Sig. (2-tailed).180.421 a K-P3 Kel_Perlakuan N Mean Rank Sum of K- 5 5.50 27.50 P3 5 5.50 27.50
Mann-Whitney U 12.500 Wilcoxon W 27.500 Z.000 Asymp. Sig. (2-tailed) 1.000 1.000 a K-P4 Kel_Perlakuan N Mean Rank Sum of K- 5 6.00 30.00 P4 5 5.00 25.00 Mann-Whitney U 10.000 Wilcoxon W 25.000 Z -.655 Asymp. Sig. (2-tailed).513.690 a \
K+P1 Kel_Perlakuan N Mean Rank Sum of K+ 5 3.40 17.00 P1 5 7.60 38.00 Mann-Whitney U 2.000 Wilcoxon W 17.000 Z -2.324 Asymp. Sig. (2-tailed).020.032 a K+P2 Kel_Perlakuan N Mean Rank Sum of K+ 5 3.80 19.00 P2 5 7.20 36.00 Mann-Whitney U 4.000 Wilcoxon W 19.000 Z -2.032 Asymp. Sig. (2-tailed).042.095 a
K+P3 Kel_Perlakuan N Mean Rank Sum of K+ 5 4.50 22.50 P3 5 6.50 32.50 Mann-Whitney U 7.500 Wilcoxon W 22.500 Z -1.225 Asymp. Sig. (2-tailed).221.310 a K+P4 Kel_Perlakuan N Mean Rank Sum of K+ 5 5.00 25.00 P4 5 6.00 30.00
Mann-Whitney U 10.000 Wilcoxon W 25.000 Z -.600 Asymp. Sig. (2-tailed).549.690 a P1P2 Kel_Perlakuan N Mean Rank Sum of P1 5 6.50 32.50 P2 5 4.50 22.50 Mann-Whitney U 7.500 Wilcoxon W 22.500 Z -1.225 Asymp. Sig. (2-tailed).221.310 a
P1P3 Kel_Perlakuan N Mean Rank Sum of P1 5 7.20 36.00 P3 5 3.80 19.00 Mann-Whitney U 4.000 Wilcoxon W 19.000 Z -2.032 Asymp. Sig. (2-tailed).042.095 a P1P4 Kel_Perlakuan N Mean Rank Sum of P1 5 7.40 37.00 P4 5 3.60 18.00 Mann-Whitney U 3.000 Wilcoxon W 18.000 Z -2.154 Asymp. Sig. (2-tailed).031.056 a
P2P3 Kel_Perlakuan N Mean Rank Sum of P2 5 6.40 32.00 P3 5 4.60 23.00 Mann-Whitney U 8.000 Wilcoxon W 23.000 Z -1.342 Asymp. Sig. (2-tailed).180.421 a P2P4 Kel_Perlakuan N Mean Rank Sum of P2 5 6.80 34.00 P4 5 4.20 21.00
Mann-Whitney U 6.000 Wilcoxon W 21.000 Z -1.678 Asymp. Sig. (2-tailed).093.222 a P3P4 Kel_Perlakuan N Mean Rank Sum of P3 5 6.00 30.00 P4 5 5.00 25.00 Mann-Whitney U 10.000 Wilcoxon W 25.000 Z -.655 Asymp. Sig. (2-tailed).513.690 a
LAMPIRAN C. Prosedur Pembuatan Bahan Uji a. Pembuatan Larutan Monosodium Glutamat (MSG) Serbuk MSG ditimbang sebanyak 100 mg dimasukkan ke dalam beaker glass ditambahkan akuades 0,2 ml diaduk menggunakan spatula Larutan MSG b. Pembuatan Larutan Vitamin C Serbuk Vitamin C ditimbang sebanyak 6,5 mg dimasukkan ke dalam beaker glass ditambahkan akuades sebanyak 0,2 ml diaduk menggunakan spatula Larutan VitaminC c. Pembuatan Larutan Vitamin E Vitamin E diambil sebanyak 0,52 mg dilarutkan dengan minyak jarak Larutan Vitamin E
Lampiran D. Rekomendasi Persetujuan Etik Penelitian Kesehatan