Lampiran 1. Hasil Identifikasi Sampel

Lampiran 1. Hasil Identifikasi Sampel 42

Lampiran 2. Kode Etik Penelitian 43

Lampiran 3. Tanaman bayam merah (Althernanthera strigosa Hask.). Tanaman bayam merah Daun bayam merah 44

Lampiran 3 (Lanjutan) Simplisia Daun Bayam Merah (Althernanthera strigosa Hask.). simplisia daun bayam merah 45

Lampiran 3 (Lanjutan) Mikroskopik Serbuk Daun Bayam Merah a b Keterangan a. serabut sklerenkim b. kristal kalsium oksalat bentuk prisma 46

Lampiran 4. Bagan Kerja Penelitian Daun Bayam Merah yang sudah dikeringkan sebanyak 1000 g Dihaluskan Ditimbang Serbuk 950 g Dilakukan karakterisasi serbuk Pemeriksaan Makroskopik Pemeriksaan Mikroskopik Penetapan Kadar Air Penetapan kadar abu total Penetapan kadar abu yang tidak larut dalam asam Penetapan kadar sari yang larut dalam etanol Penetapan kadar sari yang larut dalam air Dilakukan skrining fitokimia Pemeriksaan Alkaloida Pemeriksaan Flavonoida Pemeriksaan Tanin Pemeriksaan Saponim Pemeriksaan Steroid/ Triterpen Dibuat ekstrak Diperkolasi dengan etanol 96% Perkolat Diuapkan dengan vakum putar Dikeringkan dengan Freeze dryer Ekstrak kental Dilakukan uji antidiare Hasil 47

Lampiran 5. Perhitungan Hasil pemeriksaan Karakterisasi Serbuk. 1. Perhitungan Penetapam Kadar Air % Kadar air= a. Sampel I Berat sampel = 5,01 g Volume air = 0,4 ml volume air (ml) berat sampel (gram) x 100 % Kadar air = x100% = 7,98% b. Sampel II Berat sampel = 5,08 g Volume air = 0,4 ml Kadar air = x100% = 7,87% c. Sampel III Berat sampel = 5,12 g Volume air = 0,4 ml Kadar air = x100% = 8,78% Kadar air rata rata : % Kadar Air Rata-Rata = = 8,21% 48

2. Perhitungan Kadar Sari Yang Larut Dalam Air % Kadar Sari yang larut dalam air = x x100% a. Sampel I Berat sampel I = 5,03 gram Berat sari = 0,1237 gram Kadar sari larut air = 0,1237 5,03 x 100 x 100% = 12,29 % 20 b. Sampel II Berat sampel I = 5,08 gram Berat sari = 0,1326 gram Kadar sari larut air = 0,1236 5,08 x 100 x 100% = 13,26 % 20 c. Sampel III Berat sampel I = 5,01 gram Berat sari = 0,0908 gram Kadar sari larut air = 0,0908 5,01 x 100 x 100% = 9,06 % 20 12,29 13,26 9,06 Kadar sari larut air rata rata = 3 = 11,54% 49

3. Perhitungan Kadar Sari Yang Larut Dalam Etanol % Kadar Sari yang larut dalam etanol = x x100% a. Sampel I Berat sampel I = 5,09 gram Berat sari = 0,1798 gram Kadar sari larut air = 0,1798 5,09 x 100 x 100% = 17,53 % 20 b. Sampel II Berat sampel I = 5,04 gram Berat sari = 0,1605 gram Kadar sari larut air = 0,1605 5,04 x 100 x 100% = 15,92 % 20 c. Sampel III Berat sampel I = 5,08 gram Berat sari = 0,1654 gram Kadar sari larut air = 0,1654 5,01 x 100 20 x 100% = 16,28 % 17,53 15,92 16,28 Kadar sari larut dalam etanol rata rata = 3 = 16,37% 50

4. Perhitungan Penetapan Kadar Abu Total Kadar abu total = s bu x 100% 1. Kadar abu total I Berat sampel = 2,03 g Berat abu = 0,052 g Kadar abu total = x 100% 2. Kadar abu total II = 2,46 % Berat sampel Berat abu = 2,16 g = 0,068 g Kadar abu total = x 100% 3. Kadar abu total III = 2,7 % Berat sampel Berat abu = 2,17 g = 0,060 g Kadar abu total = x 100% = 3,76 % Kadar abu total rata-rata = 2,46 2,7 3,76 3 = 2,66 % 51

5. Perhitungan Penetapan Kadar Abu Yang Tidak Larut Dalam Asam Kadar abu total = s bu x 100% 1. Kadar abu total I Berat sampel = 2,03 g Berat abu = 0,004 g Kadar abu total = x 100% 2. Kadar abu total II = 0,19 % Berat sampel Berat abu = 2,16 g = 0,005 g Kadar abu total = x 100% 3. Kadar abu total III = 0,23 % Berat sampel Berat abu = 2,17 g = 0,004 g Kadar abu total = x 100% = 0,18 % Kadar abu total rata-rata = 0,19 0,23 0,18 3 = 0,20 % 52

Lampiran 6. Perhitungan pembuatan suspensi loperamid HCl (tablet Imodium ) Tiap tablet Imodium mengandung 2 mg loperamid HCl. Dosis dewasa 2mg 8 mg/hari ( berat dewasa untuk konversi 70kg). Dosis tikus (200g) = 0,018 x 16 mg = 0,2 mg Maka dosis loperamid Hcl untuk tikus = 1 mg/kg BB Tablet Imodium ditimbang sebanyak 20 tablet (berat = 2208 mg) 20 tablet Imodium mengandung loperamid HCl sebanyak 40 mg Loperamid HCl yang digunakan 1 mg Serbuk Imodium yang diambil = x 2208 mg = 55,2 mg Jadi serbuk Imodium sebanyak 55,2 mg mengandung loperamid HCl 1 mg. Suspensi loperamid HCl yang akan dibuat adalah 55,2 mg serbuk Imodium dilarutkan dalam 25 ml suspensi Na CMC 1%. 53

Lampiran 7. Perhitungan dosis pemberian ekstrak etanol daun bayam merah (EEDBM) dan Loperamid HCl (tablet Imodium ) 1. EEDBM yang diberikan untuk dosis 50 Konsentrasi EEDBM : 50 mg/10 ml = 5 mg/ml Berat badan tikus = 200 g Maka dosis = 50 mg x 0,2 kg = 10 mg Dosis pemberian = = 2 ml 2. EEDBM yang diberikan untuk dosis 100 Konsentrasi EEDBM : 100 mg/10 ml = 10 mg/ml Berat badan tikus = 200 g Maka dosis = 100 mg x 0,2 kg = 20 mg Dosis pemberian = = 2 ml 3. EEDBM yang diberikan untuk dosis 200 Konsentrasi EEDBM : 200 mg/10 ml = 20 mg/ml Berat badan tikus = 200 g Maka dosis = 200 mg x 0,2 kg = 40 mg Dosis pemberian = = 2 ml Konsentrasi loperamid HCl = 55,2mg/25 ml = 2,208 mg/ml Dosis yang diberikan 1 mg/kg BB Berat badan tikus = 200 g Maka dosis = 1 mg x 0,2 kg = 0,2 mg Dosis pemberian = = 0,09 ml x 100 ska la = 9 skala spuit 54

Lampiran 8. Data Penurunan Waktu Terjadinya Diare Setelah Pemberian Perlakuan Perlakuan Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig. Penurunan Waktu Suspensi Na CMC 1%.239 5.200 *.858 5.221 Suspensi Loperamid HCl 1 a. Lilliefors Significance Correction.229 5.200 *.910 5.467 Suspensi EEDBM 50.200 5.200 *.941 5.673 Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg *. This is a lower bound of the true significance..361 5.032.839 5.162.468 5.001.573 5.000 55

Descriptives N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum Suspensi Na CMC 1% BB 5 62.7400 2.56281 1.14612 59.5578 65.9222 60.00 65.40 Suspensi Loperamid HCl 1 Suspensi EEDBM 50 mg/kg Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg 5 67.3000 2.02608.90609 64.7843 69.8157 65.50 70.50 5 64.4800 2.20726.98712 61.7393 67.2207 62.20 67.80 5 102.0000 10.95445 4.89898 88.3983 115.6017 90.00 120.00 5 103.9000 9.00278 4.02616 92.7216 115.0784 99.50 120.00 Total 25 80.0840 20.04432 4.00886 71.8101 88.3579 60.00 120.00 56

ANOVA Sum of Squares df Mean Square F Sig. Between Groups 8776.214 4 2194.053 50.649.000 Within Groups 866.380 20 43.319 Total 9642.594 24 57

Dependent Variable:Waktu Tukey HSD (I) Perlakuan Suspensi Na CMC 1% BB Suspensi Loperamid HCl 1 mg/kg Suspensi EEDBM 50 Suspensi EEDBM 100 (J) Perlakuan Post Hoc Test Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval Lower Bound Upper Bound Suspensi Loperamid HCl 1-4.56000 4.16264.807-17.0162 7.8962 Suspensi EEDBM 50-1.74000 4.16264.993-14.1962 10.7162 Suspensi EEDBM 100-39.26000 * 4.16264.000-51.7162-26.8038 Suspensi EEDBM 200-41.16000 * 4.16264.000-53.6162-28.7038 Suspensi Na CMC 1% BB 4.56000 4.16264.807-7.8962 17.0162 Suspensi EEDBM 50 2.82000 4.16264.959-9.6362 15.2762 Suspensi EEDBM 100-34.70000 * 4.16264.000-47.1562-22.2438 Suspensi EEDBM 200-36.60000 * 4.16264.000-49.0562-24.1438 Suspensi Na CMC 1% BB 1.74000 4.16264.993-10.7162 14.1962 Suspensi Loperamid HCl 1-2.82000 4.16264.959-15.2762 9.6362 Suspensi EEDBM 100-37.52000 * 4.16264.000-49.9762-25.0638 Suspensi EEDBM 200-39.42000 * 4.16264.000-51.8762-26.9638 Suspensi Na CMC 1% BB 39.26000 * 4.16264.000 26.8038 51.7162 Suspensi Loperamid HCl 1 34.70000 * 4.16264.000 22.2438 47.1562 Suspensi EEDBM 50 37.52000 * 4.16264.000 25.0638 49.9762 Suspensi EEDBM 200-1.90000 4.16264.990-14.3562 10.5562 58

LSD Suspensi EEDBM 200 Suspensi Na CMC 1% BB Suspensi Loperamid HCl 1 mg/kg Suspensi EEDBM 50 Suspensi EEDBM 100 Suspensi Na CMC 1% BB 41.16000 * 4.16264.000 28.7038 53.6162 Suspensi Loperamid HCl 1 36.60000 * 4.16264.000 24.1438 49.0562 Suspensi EEDBM 50 39.42000 * 4.16264.000 26.9638 51.8762 Suspensi EEDBM 100 1.90000 4.16264.990-10.5562 14.3562 Suspensi Loperamid HCl 1-4.56000 4.16264.286-13.2431 4.1231 Suspensi EEDBM 50-1.74000 4.16264.680-10.4231 6.9431 Suspensi EEDBM 100-39.26000 * 4.16264.000-47.9431-30.5769 Suspensi EEDBM 200-41.16000 * 4.16264.000-49.8431-32.4769 Suspensi Na CMC 1% BB 4.56000 4.16264.286-4.1231 13.2431 Suspensi EEDBM 50 2.82000 4.16264.506-5.8631 11.5031 Suspensi EEDBM 100-34.70000 * 4.16264.000-43.3831-26.0169 Suspensi EEDBM 200-36.60000 * 4.16264.000-45.2831-27.9169 Suspensi Na CMC 1% BB 1.74000 4.16264.680-6.9431 10.4231 Suspensi Loperamid HCl 1-2.82000 4.16264.506-11.5031 5.8631 Suspensi EEDBM 100-37.52000 * 4.16264.000-46.2031-28.8369 Suspensi EEDBM 200-39.42000 * 4.16264.000-48.1031-30.7369 Suspensi Na CMC 1% BB 39.26000 * 4.16264.000 30.5769 47.9431 Suspensi Loperamid HCl 1 34.70000 * 4.16264.000 26.0169 43.3831 Suspensi EEDBM 50 37.52000 * 4.16264.000 28.8369 46.2031 Suspensi EEDBM 200-1.90000 4.16264.653-10.5831 6.7831 59

Suspensi EEDBM 200 Suspensi Na CMC 1% BB 41.16000 * 4.16264.000 32.4769 49.8431 Suspensi Loperamid HCl 1 36.60000 * 4.16264.000 27.9169 45.2831 Suspensi EEDBM 50 39.42000 * 4.16264.000 30.7369 48.1031 Suspensi EEDBM 100 1.90000 4.16264.653-6.7831 10.5831 *. The mean difference is significant at the 0.05 level. 60

Homogenitas Subject Perlakuan Subset for alpha = 0.05 N 1 2 Tukey HSD a Suspensi Na CMC 1% BB 5 62.7400 Suspensi EEDBM 50 5 64.4800 Suspensi Loperamid HCl 1 5 67.3000 Suspensi EEDBM 100 5 102.0000 Suspensi EEDBM 200 5 103.9000 Sig..807.990 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5,000. 61

Lampiran 9. Frekuensi Diare Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Perlakuan Statistic Df Sig. Statistic df Sig. Penurunan Waktu Suspen Na CMC 1%.231 5.200 *.881 5.314 a. Lilliefors Significance Correction Suspensi Loperamid HCl 1 Suspensi EEDBM 50 mg/kg Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg *. This is a lower bound of the true significance..473 5.001.552 5.000.367 5.026.684 5.006.367 5.026.684 5.006.367 5.026.684 5.006 62

Frekuensi Diare Descriptives N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum Suspensi Na CMC 1% 5 5.2000.83666.37417 4.1611 6.2389 4.00 6.00 Suspensi Loperamid HCl 1mg / kg Suspensi EEDBM 100 mg / kg Suspensi EEDBM 150mg / kg Suspensi EEDBM 200mg / kg 5 3.2000.83666.37417 2.1611 4.2389 2.00 4.00 5 1.2000.44721.20000.6447 1.7553 1.00 2.00 5 1.0000.00000.00000 1.0000 1.0000 1.00 1.00 5 1.0000.00000.00000 1.0000 1.0000 1.00 1.00 Total 25 2.3200 1.77294.35459 1.5882 3.0518 1.00 6.00 63

Frekuensi Diare ANOVA Sum of Squares df Mean Square F Sig. Between Groups 69.040 4 17.260 53.938.000 Within Groups 6.400 20.320 Total 75.440 24 64

Dependent Variable:Frekuensi Diare Tukey HSD Post Hoc (I) Perlakuan (J) Perlakuan Mean Difference (I- J) Suspensi Na CMC 1% Suspensi Loperamid HCl 1mg / kg Suspensi Loperamid HCl 1mg / kg Std. Error Sig. 95% Confidence Interval Lower Bound Upper Bound 2.00000 *.35777.000.9294 3.0706 Suspensi EEDBM 100 mg / kg 4.00000 *.35777.000 2.9294 5.0706 Suspensi EEDBM 150mg / kg 4.20000 *.35777.000 3.1294 5.2706 Suspensi EEDBM 200mg / kg 4.20000 *.35777.000 3.1294 5.2706 Suspensi Na CMC 1% -2.00000 *.35777.000-3.0706 -.9294 Suspensi EEDBM 100 mg / kg 2.00000 *.35777.000.9294 3.0706 Suspensi EEDBM 150mg / kg 2.20000 *.35777.000 1.1294 3.2706 Suspensi EEDBM 200mg / kg 2.20000 *.35777.000 1.1294 3.2706 Suspensi Suspensi Na CMC 1% -4.00000 *.35777.000-5.0706-2.9294 EEDBM 100 mg Suspensi Loperamid HCl 1mg / kg -2.00000 *.35777.000-3.0706 -.9294 / kg Suspensi EEDBM 150mg / kg Suspensi EEDBM 150mg / kg.20000.35777.979 -.8706 1.2706 Suspensi EEDBM 200mg / kg.20000.35777.979 -.8706 1.2706 Suspensi Na CMC 1% -4.20000 *.35777.000-5.2706-3.1294 Suspensi Loperamid HCl 1mg / kg -2.20000 *.35777.000-3.2706-1.1294 65

LSD Suspensi EEDBM 200mg / kg Suspensi Na CMC 1% Suspensi Loperamid HCl 1mg / kg Suspensi EEDBM 100 mg / kg -.20000.35777.979-1.2706.8706 Suspensi EEDBM 200mg / kg.00000.35777 1.000-1.0706 1.0706 Suspensi Na CMC 1% -4.20000 *.35777.000-5.2706-3.1294 Suspensi Loperamid HCl 1mg / kg -2.20000 *.35777.000-3.2706-1.1294 Suspensi EEDBM 100 mg / kg -.20000.35777.979-1.2706.8706 Suspensi EEDBM 150mg / kg.00000.35777 1.000-1.0706 1.0706 Suspensi Loperamid HCl 1mg / kg 2.00000 *.35777.000 1.2537 2.7463 Suspensi EEDBM 100 mg / kg 4.00000 *.35777.000 3.2537 4.7463 Suspensi EEDBM 150mg / kg 4.20000 *.35777.000 3.4537 4.9463 Suspensi EEDBM 200mg / kg 4.20000 *.35777.000 3.4537 4.9463 Suspensi Na CMC 1% -2.00000 *.35777.000-2.7463-1.2537 Suspensi EEDBM 100 mg / kg 2.00000 *.35777.000 1.2537 2.7463 Suspensi EEDBM 150mg / kg 2.20000 *.35777.000 1.4537 2.9463 Suspensi EEDBM 200mg / kg 2.20000 *.35777.000 1.4537 2.9463 Suspensi Suspensi Na CMC 1% -4.00000 *.35777.000-4.7463-3.2537 EEDBM 100 mg Suspensi Loperamid HCl 1mg / kg -2.00000 *.35777.000-2.7463-1.2537 / kg Suspensi EEDBM 150mg / kg Suspensi EEDBM 150mg / kg.20000.35777.582 -.5463.9463 Suspensi EEDBM 200mg / kg.20000.35777.582 -.5463.9463 Suspensi Na CMC 1% -4.20000 *.35777.000-4.9463-3.4537 Suspensi Loperamid HCl 1mg / kg -2.20000 *.35777.000-2.9463-1.4537 66

Suspensi EEDBM 200mg / kg Suspensi EEDBM 100 mg / kg -.20000.35777.582 -.9463.5463 Suspensi EEDBM 200mg / kg.00000.35777 1.000 -.7463.7463 Suspensi Na CMC 1% -4.20000 *.35777.000-4.9463-3.4537 Suspensi Loperamid HCl 1mg / kg -2.20000 *.35777.000-2.9463-1.4537 Suspensi EEDBM 100 mg / kg -.20000.35777.582 -.9463.5463 Suspensi EEDBM 150mg / kg.00000.35777 1.000 -.7463.7463 *. The mean difference is significant at the 0.05 level. 67

Homogeneous Subsets Subset for alpha = 0.05 N Perlakuan 1 2 3 Tukey HSD a Suspensi EEDBM 150mg / kg 5 1.0000 Suspensi EEDBM 200mg / kg 5 1.0000 Suspensi EEDBM 100 mg / kg 5 1.2000 Suspensi Loperamid HCl 1mg / kg 5 3.2000 Suspensi Na CMC 1% 5 5.2000 Sig..979 1.000 1.000 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5,000. 68

Lampiran 10. Lama Terjadinya Diare Perlakuan Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig. Penurunan Waktu Suspen Na CMC 1%.244 5.200 *.944 5.697 a. Lilliefors Significance Correction Suspensi Loperamid HCl 1 Suspensi EEDBM 50 mg/kg Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg *. This is a lower bound of the true significance..199 5.200 *.966 5.848.270 5.200 *.855 5.210.229 5.200 *.968 5.860.273 5.200 *.845 5.180 69

lama terjadinya diare(menit) Descriptives N Mean Std. Deviation Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum Suspensi Na CMC 1% 5 265.00 27.009 12.079 231.46 298.54 230 297 Suspensi Loperamid 1 Suspensi EEDBM 50 Suspensi EEDBM 100 Suspensi EEDBM 200 5 238.80 30.614 13.691 200.79 276.81 193 277 5 196.80 18.620 8.327 173.68 219.92 170 212 5 181.20 21.312 9.531 154.74 207.66 155 213 5 191.80 17.413 7.787 170.18 213.42 178 220 Total 25 214.72 38.983 7.797 198.63 230.81 155 297 70

lama terjadinya diare(menit) ANOVA Sum of Squares Df Mean Square F Sig. Between Groups 25389.840 4 6347.460 11.454.000 Within Groups 11083.200 20 554.160 Total 36473.040 24 71

Dependent Variable:lama terjadinya diare(menit) Tukey HSD (I) Perlakuan Suspensi Na CMC 1% Suspensi Loperamid 1 Suspensi EEDBM 50 mg/kg (J) Perlakuan Post Hoc Suspensi Loperamid 1 mg/kg Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval Lower Bound Upper Bound 26.200 14.888.423-18.35 70.75 Suspensi EEDBM 50 68.200 * 14.888.002 23.65 112.75 Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg 83.800 * 14.888.000 39.25 128.35 73.200 * 14.888.001 28.65 117.75 Suspensi Na CMC 1% -26.200 14.888.423-70.75 18.35 Suspensi EEDBM 50 42.000 14.888.071-2.55 86.55 Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg 57.600 * 14.888.008 13.05 102.15 47.000 * 14.888.036 2.45 91.55 Suspensi Na CMC 1% -68.200 * 14.888.002-112.75-23.65 Suspensi Loperamid 1 mg/kg Suspensi EEDBM 100 mg/kg -42.000 14.888.071-86.55 2.55 15.600 14.888.830-28.95 60.15 72

Suspensi EEDBM 100 Suspensi EEDBM 200 Suspensi EEDBM 200 mg/kg 5.000 14.888.997-39.55 49.55 Suspensi Na CMC 1% -83.800 * 14.888.000-128.35-39.25 Suspensi Loperamid 1 mg/kg -57.600 * 14.888.008-102.15-13.05 Suspensi EEDBM 50-15.600 14.888.830-60.15 28.95 Suspensi EEDBM 200 mg/kg -10.600 14.888.951-55.15 33.95 Suspensi Na CMC 1% -73.200 * 14.888.001-117.75-28.65 Suspensi Loperamid 1 mg/kg -47.000 * 14.888.036-91.55-2.45 Suspensi EEDBM 50-5.000 14.888.997-49.55 39.55 Suspensi EEDBM 100 mg/kg LSD Suspensi Na CMC 1% Suspensi Loperamid 1 mg/kg Suspensi Loperamid 1 10.600 14.888.951-33.95 55.15 26.200 14.888.094-4.86 57.26 Suspensi EEDBM 50 68.200 * 14.888.000 37.14 99.26 Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg 83.800 * 14.888.000 52.74 114.86 73.200 * 14.888.000 42.14 104.26 Suspensi Na CMC 1% -26.200 14.888.094-57.26 4.86 Suspensi EEDBM 50 42.000 * 14.888.011 10.94 73.06 Suspensi EEDBM 100 mg/kg 57.600 * 14.888.001 26.54 88.66 73

Suspensi EEDBM 50 mg/kg Suspensi EEDBM 100 Suspensi EEDBM 200 Suspensi EEDBM 200 mg/kg 47.000 * 14.888.005 15.94 78.06 Suspensi Na CMC 1% -68.200 * 14.888.000-99.26-37.14 Suspensi Loperamid 1 mg/kg Suspensi EEDBM 100 mg/kg Suspensi EEDBM 200 mg/kg -42.000 * 14.888.011-73.06-10.94 15.600 14.888.307-15.46 46.66 5.000 14.888.740-26.06 36.06 Suspensi Na CMC 1% -83.800 * 14.888.000-114.86-52.74 Suspensi Loperamid 1 mg/kg -57.600 * 14.888.001-88.66-26.54 Suspensi EEDBM 50-15.600 14.888.307-46.66 15.46 Suspensi EEDBM 200 mg/kg -10.600 14.888.485-41.66 20.46 Suspensi Na CMC 1% -73.200 * 14.888.000-104.26-42.14 Suspensi Loperamid 1 mg/kg Suspensi EEDBM 100 mg/kg *. The mean difference is significant at the 0.05 level. -47.000 * 14.888.005-78.06-15.94 Suspensi EEDBM 50-5.000 14.888.740-36.06 26.06 10.600 14.888.485-20.46 41.66 74

Homogeneous Subsets Perlakuan N Subset for alpha = 0.05 1 2 3 Tukey HSD a Suspensi EEDBM 100 5 181.20 Suspensi EEDBM 200 5 191.80 Suspensi EEDBM 50 Suspensi Loperamid 1 5 196.80 196.80 5 238.80 238.80 Suspensi Na CMC 1% 5 265.00 Sig..830.071.423 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 5,000. 75