LAPORAN AKHIR INSENTIF PENINGKATAN KEMAMPUAN PENELITI DAN PEREKAYASA

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1 KODE JUDUL : X.175 LAPORAN AKHIR INSENTIF PENINGKATAN KEMAMPUAN PENELITI DAN PEREKAYASA PEMANTAUAN RESISTENSI Plutella xylostella TERHADAP INSEKTISIDA YANG UMUM DIGUNAKAN OLEH PETANI KUBIS DI DATARAN TINGGI SULAWESI SELATAN SEBAGAI DASAR PEMILIHAN INSEKTISIDA YANG TEPAT KEMENTERIAN PERTANIAN BADAN PENELITIAN DAN PENGEMBANGAN PERTANIAN Peneliti/ Perekayasa : 1. Dr. Ir. Laksminiwati Prabaningrum, MS 2. Ir. Tinny S. Uhan, MS 3. Ir. Uvan Nurwahidah, MS INSENTIF PENINGKATAN KEMAMPUAN PENELITI DAN PEREKAYASA KEMENTERIAN RISET DAN TEKNOLOGI

2 LEMBAR IDENTITAS DAN PENGESAHAN Judul Kegiatan Pemantauan Resistensi Plutella xylostella terhadap Insektisida yang Umum Digunakan Oleh Petani Kubis di Dataran Tinggi Sulawesi Selatan Sebagai Dasar Pemilihan Insektisida yang Tepat Fokus Bidang Prioritas 1. Teknologi Pangan 2. Teknologi Kesehatan dan Obat 3. Teknologi Enerji 4. Teknologi Transportasi 5. Teknologi Informatika dan Komunikasi 6. Teknologi Pertahanan dan Keamanan 7. Teknologi Material Kode Produk Target 1.3. Kode Kegiatan Lokasi Penelitian Provinsi Sulawesi Selatan Penelitian Tahun ke 1 (satu) Keterangan Lembaga Pelaksana/ Pengelola Penelitian A. Lembaga Pelaksanaan Penelitian Nama Peneliti Utama Dr. Laksminiwati Prabaningrum, Ir. MS. Nama Lembaga/ Institusi Balai Penelitian Tanaman Sayuran Unit Organisasi Kementerian Pertanian Alamat Jl. Tangkuban Parahu No. 517, Lembang, Bandung Barat Telepon/ HP / Faksimile laksminiwati@yahoo.co.id 1

3 B. Lembaga lain yang terlibat (dapat lebih dari satu) Nama Pimpinan Ir. Firdaus Hasan, MS Nama Lembaga UPTD Balai Proteksi Tanaman Pangan dan Hortikultura Provinsi Sulawesi Selatan Alamat Jl. dr. Sam Ratulangi No. 69, Maros 90511, Sulawesi Selatan Telepon/ HP / Faksimile - - Jangka Waktu Kegiatan : 8 (delapan) bulan B i a y a : Rp ,- Menyetujui : Pj. Kepala Balai Penelitian Tanaman Sayuran, Peneliti Utama, Dr. Liferdi, SP., MSi NIP Dr.Laksminiwati Prabaningrum NIP

4 EXECUTIVE SUMMARY Plutella xylostella is a key pest of cabbage that has an ability to quickly develop to be resistant to insecticides. Therefore, integrated resistance management (IRM) is an important programme to be done. A tactic in IRM is periodically and sustainable resistance monitoring. Research on monitoring of P. xylostella xylostella resistance to insecticides used by farmers on cabbage in South Sulawesi as a base for choosing the effective insecticides had been conducted from February until August 2012 at Anggeraja (590 m asl) and Baroko subdistrcts (1,040 m asl), Enrekang District and Tombolo Pao (1,600 m asl) and Tinggi Moncong Subdistricts, Gowa District, South Sulawesi Province. In the early experiment, a survey with cabbage farmers as respondents was carried out to determine kind of insecticide used and farmers behaviour in applicating insecticide for controlling P. xylostella. Toxicity test was carried out at Division of Biological Control, Food and Horticulture Crops Protection Institute in South Sulawesi Province. Third instar of P. xylostella larvae were used in the experiment. Toxicity test used Protocol No. 7, Proposed Insecticide/ Acaricide Susceptibility Test created by Insecticide Resistance Action Committee. Larvae mortality data and their correlation with formulation concentration of insecticides tested were analysed used Probit Analysis according to Finney, to determine the LC 50. Interprestation of P. xylostella resistance to insecticides tested was got by comparing LC 50 of insecticides tested with LC 50 for the susceptible strain. LC 50 of the susceptible strain of P. xylostella was the lowest LC 50 among insecticides tested. Insect is resistant if the LC 50 4 times to the LC 50 for susceptible one. Result of the survey showed that there were eight kinds of insecticides used by farmers in cabbage cultivation. Toxicity test of the insecticides showed that there were varies susceptibility of P. xylostella larvae from different locations and the larvae had been developed to resistant to insecticides. The budget of the research was IDR 150,000,000 (one hundred and fifty millions rupiahs). The money was managed by Research Collaboration Division in IVEGRI, and given to the researcher in three terms. The research 3

5 produced an information that will be published in Journal of Horticulture, ICHORD, AARD. At the moment, the draft has been submitted to the editors of the journal. The money in the first term was too small, so that it disturbed the activity. Filling on line report was difficult because the database memory was too small. Results of the research showed that P. xylostella from Anggeraja Subdistrict had been resistant to Beta-cyfluthrin, Lambda-cyhalothrin, Lambda-cyhalothrin + Chlorantraniliprol, Permethrin, Chlorantraniliprol, B. thuringiensis and Tiametoxam + Chlorantraniliprol, but it was still susceptible to Spinosad. P. xylostella from Baroko Subdistrct had been resistant to Betacyfluthrin, Lambda-cyhalothrin, Permethrin, Chlorantraniliprol, and B. thuringiensis, but it was still susceptible to Lambda-cyhalothrin + Chlorantraniliprol, Spinosad, and and Tiametoxam + Chlorantraniliprol. P. xylostella from Tombolo Pao Subdistrict had been resistant to Beta-cyfluthrin, Lambda-cyhalothrin, Permethrin, Chlorantraniliprol, and Tiametoxam + Chlorantraniliprol, but it was still susceptible to Lambda-cyhalothrin + Chlorantraniliprol, Spinosad, and B. thuringiensis. P. xylostella from Tinggi Moncong Subdistrct had been resistant to Beta-cyfluthrin, Lambdacyhalothrin, Lambda-cyhalothrin + Chlorantraniliprol, Permethrin, Chlorantraniliprol, and Tiametoxam + Chlorantraniliprol, but it was still susceptible to Spinosad and B. thuringiensis. Based on the result, all ineffective insecticides in a sub-district can not be used in a periode of 1-2 years and have to be replaced with other effective insecticides that have different mode of action code. Insecticide resistance management (IRM) will be determined as a programme of The Food and Horticultural Crops Protection Institute (FHCPI) in South Sulawesi. A protocol of toxicity test will be created to support the programme. The institution also can develop IRM for other pests using the protocol. The activities in the research were supported by extension workers and pest observers in the location. They worked together and took experiences in toxicology tests. The research had been used as a training forum. Result of the research showed that there were several kinds of insecticides that still effective and not efffective for controlling P. xylostella 4

6 larvae. The information was delivered to The Food and Horticultural Crops Protection Institute and Agriculture Office in South Sulawesi in order to create a strategy for determining the right and effective insecticides to control P. xylostella in every location. The Food and Horticultural Crops Protection Institute in South Sulawesi determines IRM as a routine programme in the institute. All stakeholders have to have an agrreement for regulating the use of pesticide in order to delay pests resistance. 5

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LAPORAN AKHIR INSENTIF PENINGKATAN KEMAMPUAN PENELITI DAN PEREKAYASA

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