Lampiran 1. Kode etik penelitian

Lampiran 1. Kode etik penelitian 38

Lampiran 2. Skema Penelitian 1. Pembuatan Seduhan Teh Hijau dan Teh Hitam Ditimbang teh hijau dan teh hitam sebanyak 1750 /kg, 3500 /kg dan 7000 /kg Seduhan teh dosis1750 /kg, 3500 /kg dan 7000 /kg Diseduh dengan air mendidih sebanyak 30 ml selama 3 menit lalu disaring menggunakan kain flanel 2. Pengujian metode defekasi 36 ekor mencit Diberi penandaan pada masing-masingekor mencit Ditimbang dan catat berat badanmencit Diberikan perlakuansecara per oral: suspensi CMC 1%, teh Hijau, teh Hitam dan suspensiloperamid HCl 2 30 menit kemudian diberikan Oleum ricini 0,5 ml secara p.o Diamati selama enam jam dengan interval waktu tiap 30 menit. Waktu mulai diare (menit) Frekuensi diare ( x/menit) Bobot feses () 39

3. Pengujian metode antisekretori 36 ekor mencit Ditimbang dan catat berat badanmencit Diberikan perlakuansecara per oral : suspensi CMC 1%, teh Hijau, teh Hitam dan suspensiloperamid HCl 2 30 menit kemudian diberikan Oleum ricini 0,5 mlsecara p.o dibiarkan kembali selama 30 menit, Kemudian semua mencit dikorbankan dengan cara dislokasi leher dilakukan pembedahan. Saluran gastroinstetinal dari phylorus sampai caecum dipotong isinya dikeluarkan dan ditampung dalam gelas ukur untuk ditimbang volumenya Volume isi usus () 40

Lampiran 3. Contoh perhitungan dosis a) Perhitungan dosis CMC Na 1% = = 10 /ml Perhitungan CMC Na 1% pada mencit 25 g = x 25 g = 0,25 ml Volume yang diberikan = 0,25 ml b) Perhitungan dosis Lodia tablet Tiap tablet Lodia mengandung 2 Loperamid HCl Dosis dewasa 2 8 /hari dan tidak lebih dari 16 /hari (berat dewasa untuk konversi 70 Kg). Dosis untuk mencit 20 g yaitu : (2 8 ) x 0,0026 = 0,0052 0,0208 /hari dan tidak lebih dari 0,04616 /Kg. Berat serbuk Lodia yang akan diambil yaitu dari 20 tablet lodia di gerus dan ditimbang = 2136. Dari 20 tablet mengandung loperamid HCl sebanyak 40. Dosis loperamid HCl yang digunakan adalah dosis maksimum yaitu : = 0,0416 /20 g mencit = 2,08 /kg Loperamid HCl yang digunakan adalah : 2,08 /kg 41

Maka serbuk loperamid yang di gunakan adalah : = x 2136 = 111,07 Dalam serbuk lodia sebanyak 111,07 mengandung loperamid HCl sebanyak 2,08. Suspensi loperamid HCl yang akan dibuat adalah 111,07 serbuk lodia dilarutkan dalam 10 ml suspensi CMC 1 %. c) Perhitungan dosis seduhan teh hijau dan teh hitam 1. Untuk dosis 1750 /kg Jumlah yang diberikan = x 25 g = 43,75 Jumlah seduhan yang diberikan = x 30 ml = 0,75 ml 2. Untuk dosis 3500 /kg Jumlah yang diberikan = x 25 g = 87,5 Jumlah seduhan yang diberikan = x 30 ml = 0,75 ml 3. Untuk dosis 7000 /kg Jumlah yang diberikan = x 25 g = 175 Jumlah seduhan yang diberikan = x 30 ml = 0,75 ml 42

Lampiran 4. Waktu terjadinya diare Perlakuan Hewan 1 2 3 4 Jumlah Ratarata (menit) Oleum Ricini 33,5 35,7 37,5 36,2 142,9 35,7 Suspensi CMC 1% 37 35,3 37,5 35,4 145,2 36,3 Suspensi loperamid 2 62,03 60,5 65,2 60,2 247,93 61,9 STHJ 1750 /kgbb 40,4 45,2 40,5 44,3 170,4 42,6 STHJ 3500 /kgbb 47,5 50,2 46,3 45,5 189,5 47,3 STHJ 7000 /kgbb 57,3 60,3 60,6 65,5 243,7 60,9 STHT 1750 /kgbb 40 39,4 45,5 40,4 165,3 41,3 STHT 3500 /kgbb 47,5 47,5 42,3 45,5 182,8 45,7 STHT 7000 /kgbb 50,4 48,4 47,5 49,3 195,6 48,9 43

Lampiran 5. Frekuensi diare Menit ke- Perlakuan Hewan Jumlah 30 60 120 150 180 210 240 270 300 330 360 390 Oleum ricini Suspensi CMC 1 % Suspensi Loperamid 2 STHJ 1750 /kgbb STHJ 3500 /kg STHJ 7000 /kg STHT 1750 /kg STHT 3500 /kg STHT 7000 /kg 1 0 2 2 1 1 2 0 0 1 0 0 0 9 2 0 3 2 2 0 0 2 2 1 2 0 0 14 3 0 2 2 1 0 2 1 2 1 0 0 0 11 4 0 2 2 3 2 1 1 0 1 0 1 0 13 1 0 3 2 1 1 0 0 1 1 0 0 0 9 2 0 3 2 2 0 0 2 2 2 0 1 0 14 3 0 3 2 0 0 2 1 2 1 2 0 0 13 4 0 2 2 1 2 1 1 0 1 0 0 0 10 1 0 0 2 2 1 0 1 0 0 1 0 0 7 2 0 0 1 1 0 0 0 1 1 0 0 0 4 3 0 0 1 1 0 1 0 1 1 0 0 0 5 4 0 0 2 1 0 1 1 0 1 0 0 0 6 1 0 2 2 1 2 0 0 1 0 1 0 0 9 2 0 1 2 1 0 0 1 1 1 0 0 0 7 3 0 2 0 2 0 1 0 1 0 1 1 0 8 4 0 2 1 0 1 0 0 0 1 0 0 0 5 1 0 2 1 1 2 0 0 1 0 1 0 0 8 2 0 1 2 1 0 0 1 1 1 0 0 0 7 3 0 1 0 2 0 1 0 1 0 0 1 0 6 4 0 2 1 0 1 0 0 0 1 0 0 0 5 1 0 1 2 1 0 0 1 0 0 1 0 0 6 2 0 0 2 0 1 0 0 1 1 0 0 0 5 3 0 0 1 2 0 1 0 1 1 1 0 0 7 4 0 0 2 0 1 1 0 0 1 0 0 0 5 1 0 2 1 1 1 0 0 1 0 0 0 0 6 2 0 2 2 1 0 0 1 0 1 0 1 0 8 3 0 3 2 0 0 1 1 1 1 1 0 0 10 4 0 1 2 1 1 1 1 0 1 0 0 0 8 1 0 2 2 2 1 1 0 0 1 0 1 0 10 2 0 1 1 1 1 0 0 1 1 1 0 0 7 3 0 2 1 0 1 0 1 0 1 0 0 1 7 4 0 2 1 1 0 1 0 0 0 1 0 0 6 0 2 1 0 1 1 0 0 1 0 1 0 7 2 0 1 1 0 1 0 0 1 1 1 0 0 6 3 0 1 1 0 1 0 0 0 1 0 0 1 5 4 0 1 1 2 0 1 1 0 1 0 0 0 7 44

Lampiran 6. Bobot feses Bobot feses () Perlakuan Hewan Jumlah 30 60 120 150 180 210 240 270 300 330 360 390 Oleum Ricini Suspensi CMC 1 % Susp. Loperamid 2 STHJ 1750 /kgbb STHJ 3500 /kgbb STHJ 7000 /kgbb STHT 1750 /kgbb STHT 3500 /kgbb STHT 7000 /kgbb 1 0 362 228 253 187 254 0 0 115 0 0 0 1399 2 0 244 234 256 0 0 246 224 124 70 0 0 1398 3 0 255 167 235 0 220 213 108 100 0 0 0 1298 4 0 273 153 150 124 115 108 0 90 0 1 0 1014 1 0 287 148 109 220 0 0 160 165 0 0 0 1089 2 0 376 186 278 0 0 155 102 80 0 50 0 1227 3 0 190 278 0 0 254 146 167 100 110 0 0 1245 4 0 189 256 189 234 95 90 0 100 0 0 0 1153 1 0 0 298 210 160 0 125 0 0 90 0 0 883 2 0 0 134 160 0 0 0 140 110 0 0 0 544 3 0 0 120 160 0 200 0 110 80 0 0 0 670 4 0 0 245 180 0 185 120 0 100 0 0 0 830 1 0 276 240 180 181 0 0 100 0 60 0 0 1037 2 0 200 275 170 0 0 145 110 80 0 0 0 980 3 0 230 0 230 0 180 0 175 0 90 100 0 1005 4 0 280 245 0 110 0 0 0 80 0 0 0 715 1 0 250 161 120 130 0 0 60 0 50 0 0 771 2 0 178 210 130 0 0 120 180 150 0 0 0 968 3 0 250 0 160 0 119 0 130 0 0 90 0 749 4 0 270 260 0 130 0 0 0 100 0 0 0 760 1 0 200 210 160 0 0 100 0 0 90 0 0 760 2 0 0 180 0 140 0 0 130 100 0 0 0 550 3 0 0 187 200 0 189 0 150 140 100 0 0 966 4 0 0 210 0 180 170 0 0 145 0 0 0 705 1 0 290 120 130 130 0 0 1 0 0 0 0 671 2 0 240 230 178 0 0 160 0 150 0 70 0 1028 3 0 280 240 0 0 189 130 110 112 100 0 0 1161 4 0 180 260 110 180 100 110 0 90 0 0 0 1030 1 0 290 170 200 120 100 0 0 110 0 100 0 1090 2 0 250 230 180 110 0 0 100 90 70 0 0 1030 3 0 259 155 0 120 0 100 0 110 0 0 90 834 4 0 245 160 140 0 170 0 0 0 1 0 0 716 0 210 200 0 170 140 0 0 150 0 70 0 940 2 0 220 180 0 130 0 0 120 100 90 0 0 840 3 0 180 210 0 120 0 0 0 110 0 0 90 710 4 0 200 155 160 0 130 140 0 100 0 0 0 885 45

Lampiran 7. Volume isi usus antisekretori Perlakuan Hewan 1 2 3 4 Jumlah Ratarata () Oleum Ricini 500 400 520 530 1950 487,5 Suspensi CMC 1% 480 500 480 480 1940 485 Suspensi loperamid 2 320 355 320 346 1341 335,2 STHJ 1750 /kg 376 380 410 410 1576 394 STHJ 3500 /kg 400 340 328 405 1473 368,2 STHJ 7000 /kg 320 390 340 315 1365 341,2 STHT 1750 /kg 430 410 359 450 1649 412,2 STHT 3500 /kg 400 378 450 387 1615 403,7 STHT 7000 /g 360 368 400 350 1478 369,5 46

Lampiran 8. Gambar Sampel dan Hewan uji Gambar 1. Sampel Teh Hitam Kemasan Gambar 2. Sampel Teh Hijau Kemasan Gambar 3. Seduhan Teh Hitam Kemasan Gambar 4. Seduhan Teh Hijau Kemasan Gambar 5. Hewan uji Gambar 6.Kandang Uji antidiare 47

Lampiran 9. Tabel Volume maksimal dan Konversi Volume Maksimal Larutan Pemberian Pada Binatang V0lume Maksimal (ml) i.v i.m i.p s.c p.0 Mencit 20-30 g Tikus 100 g Marm0t 250 g Kelinci 2,5 kg kucing 3 kg Hamster 50 g Anjing 5 kg Merpati 300 g 0,5 0,1 0,1 0,5-1,0 1,0 1,0 0,1 2,0-5,0 2,0-5,0 5,0 0,25 2,0-5,0 5,0 10,0 41552 0,5 44105,0 41552,0 20,0 41552,0 1,0 44105,0 41552,0 50,0 0,0 0,2 0,4 0,9 1,0 44105,0 5,0 20-50 41552,0 100,0 2,0 0,5 2,0 2,0 10,0 Konversi Dosis antar Jenis Subjek Uji Mencit Tikus Marm0t Kelinci kucing Kera Anjing manusia 20 g 200 g 400 g 1,5 kg 2 kg 4 kg 12 kg 70 kg Mencit 20 g Tikus 200 g Marm0t 400 g Kelinci 1,5 kg kucing 2 kg Kera 4 kg Anjing 12 kg manusia 70 kg 1,0 7,0 12,29 27,8 29,7 64,1 142,2 387,9 0,14 1,0 1,74 3,3 4,2 9,2 17,8 56,0 0,08 0.57 1,0 2,3 2,4 5,2 10,2 31,5 0,04 0,25 0,44 1,0 1,1 2,4 4,5 14,2 0,03 0,23 0,41 0,92 1,0 2,2 4,1 13,0 0,016 0,11 0,19 0,42 0,45 1,0 1,9 6,10 0,008 0,06 0,10 0,22 0,24 0,5 1,0 3,10 0,0026 0,02 0,31 0,07 1,01 0,16 0,32 1,20 48

Lampiran 10. Data Analisis Statistik Waktu mulai Diare Oneway Descriptives Waktu_Mulai_Diare 95% Confidence Interval for Mean Std. Lower Upper N Mean Deviation Std. Error Bound Bound Minimum Maximum oleum ricini 4 35,7250 1,66608,83304 33,0739 38,3761 33,50 37,50 CMC 1% 4 36,3000 1,11654,55827 34,5233 38,0767 35,30 37,50 Suspensi Loperamid HCl 4 61,9825 2,28981 1,14490 58,3389 65,6261 60,20 65,20 2 STHJ 1750 /kg 4 42,6000 2,50998 1,25499 38,6061 46,5939 40,40 45,20 STHJ 3500 /kg 4 47,3750 2,05487 1,02744 44,1052 50,6448 45,50 50,20 STHJ 7000 /kg 4 60,9250 3,39448 1,69724 55,5236 66,3264 57,30 65,50 STHT 1750 /kg 4 41,3250 2,81351 1,40675 36,8481 45,8019 39,40 45,50 STHT 3500 /kg 4 45,7000 2,45493 1,22746 41,7937 49,6063 42,30 47,50 STHT 7000 /kg 4 48,9000 1,24097,62048 46,9253 50,8747 47,50 50,40 Total 36 46,7592 9,26792 1,54465 43,6234 49,8950 33,50 65,50 ANOVA Waktu_Mulai_Diare Sum of Squares df Mean Square F Sig. Between Groups 2865,923 8 358,240 68,902,000 Within Groups 140,380 27 5,199 Total 3006,303 35 49

Lampiran (lanjutan) Post Hoc Tests Multiple Comparisons Dependent Variable:Waktu_Mulai_Diare (I) Percobaan (J) Percobaan Mean 95% Confidence Interval Difference (I- J) Std. Error Sig. Lower Bound Upper Bound LSD oleum ricini CMC 1% -,57500 1,61233,724-3,8832 2,7332 Susp. Loperamid HCl 2-26,25750 * 1,61233,000-29,5657-22,9493 STHJ 1750 /kg -6,87500 * 1,61233,000-10,1832-3,5668 STHJ 3500 /kg -11,65000 * 1,61233,000-14,9582-8,3418 STHJ 7000 /kg -25,20000 * 1,61233,000-28,5082-21,8918 STHT 1750 /kg -5,60000 * 1,61233,002-8,9082-2,2918 STHT 3500 /kg -9,97500 * 1,61233,000-13,2832-6,6668 STHT 7000 /kg -13,17500 * 1,61233,000-16,4832-9,8668 CMC 1% oleum ricini,57500 1,61233,724-2,7332 3,8832 Susp. Loperamid HCl 2-25,68250 * 1,61233,000-28,9907-22,3743 STHJ 1750 /kg -6,30000 * 1,61233,001-9,6082-2,9918 STHJ 3500 /kg -11,07500 * 1,61233,000-14,3832-7,7668 STHJ 7000 /kg -24,62500 * 1,61233,000-27,9332-21,3168 STHT 1750 /kg -5,02500 * 1,61233,004-8,3332-1,7168 STHT 3500 /kg -9,40000 * 1,61233,000-12,7082-6,0918 STHT 7000 /kg -12,60000 * 1,61233,000-15,9082-9,2918 Susp. Loperamid HCl 2 oleum ricini 26,25750 * 1,61233,000 22,9493 29,5657 CMC 1% 25,68250 * 1,61233,000 22,3743 28,9907 STHJ 1750 /kg 19,38250 * 1,61233,000 16,0743 22,6907 STHJ 3500 /kg 14,60750 * 1,61233,000 11,2993 17,9157 STHJ 7000 /kg 1,05750 1,61233,517-2,2507 4,3657 STHT 1750 /kg 20,65750 * 1,61233,000 17,3493 23,9657 STHT 3500 /kg 16,28250 * 1,61233,000 12,9743 19,5907 STHT 7000 /kg 13,08250 * 1,61233,000 9,7743 16,3907 STHJ 1750 /kg oleum ricini 6,87500 * 1,61233,000 3,5668 10,1832 CMC 1% 6,30000 * 1,61233,001 2,9918 9,6082 50

Susp. Loperamid HCl 2-19,38250 * 1,61233,000-22,6907-16,0743 STHJ 3500 /kg -4,77500 * 1,61233,006-8,0832-1,4668 STHJ 7000 /kg -18,32500 * 1,61233,000-21,6332-15,0168 STHT 1750 /kg 1,27500 1,61233,436-2,0332 4,5832 STHT 3500 /kg -3,10000 1,61233,065-6,4082,2082 STHT 7000 /kg -6,30000 * 1,61233,001-9,6082-2,9918 STHJ 3500 /kg oleum ricini 11,65000 * 1,61233,000 8,3418 14,9582 CMC 1% 11,07500 * 1,61233,000 7,7668 14,3832 Susp.Loperamid HCl 2-14,60750 * 1,61233,000-17,9157-11,2993 STHJ 1750 /kg 4,77500 * 1,61233,006 1,4668 8,0832 STHJ 7000 /kg -13,55000 * 1,61233,000-16,8582-10,2418 STHT 1750 /kg 6,05000 * 1,61233,001 2,7418 9,3582 STHT 3500 /kg 1,67500 1,61233,308-1,6332 4,9832 STHT 7000 /kg -1,52500 1,61233,353-4,8332 1,7832 STHJ 7000 /kg oleum ricini 25,20000 * 1,61233,000 21,8918 28,5082 CMC 1% 24,62500 * 1,61233,000 21,3168 27,9332 Susp.Loperamid HCl 2-1,05750 1,61233,517-4,3657 2,2507 STHJ 1750 /kg 18,32500 * 1,61233,000 15,0168 21,6332 STHJ 3500 /kg 13,55000 * 1,61233,000 10,2418 16,8582 STHT 1750 /kg 19,60000 * 1,61233,000 16,2918 22,9082 STHT 3500 /kg 15,22500 * 1,61233,000 11,9168 18,5332 STHT 7000 /kg 12,02500 * 1,61233,000 8,7168 15,3332 STHT 1750 /kg oleum ricini 5,60000 * 1,61233,002 2,2918 8,9082 CMC 1% 5,02500 * 1,61233,004 1,7168 8,3332 Susp. Loperamid HCl 2-20,65750 * 1,61233,000-23,9657-17,3493 STHJ 1750 /kg -1,27500 1,61233,436-4,5832 2,0332 STHJ 3500 /kg -6,05000 * 1,61233,001-9,3582-2,7418 STHJ 7000 /kg -19,60000 * 1,61233,000-22,9082-16,2918 STHT 3500 /kg -4,37500 * 1,61233,011-7,6832-1,0668 STHT 7000 /kg -7,57500 * 1,61233,000-10,8832-4,2668 STHT 3500 /kg oleum ricini 9,97500 * 1,61233,000 6,6668 13,2832 CMC 1% 9,40000 * 1,61233,000 6,0918 12,7082 51

Susp. Loperamid HCl 2-16,28250 * 1,61233,000-19,5907-12,9743 STHJ 1750 /kg 3,10000 1,61233,065 -,2082 6,4082 STHJ 3500 /kg -1,67500 1,61233,308-4,9832 1,6332 STHJ 7000 /kg -15,22500 * 1,61233,000-18,5332-11,9168 STHT 1750 /kg 4,37500 * 1,61233,011 1,0668 7,6832 STHT 7000 /kg -3,20000 1,61233,057-6,5082,1082 STHT 7000 /kg oleum ricini 13,17500 * 1,61233,000 9,8668 16,4832 CMC 1% 12,60000 * 1,61233,000 9,2918 15,9082 Susp. Loperamid HCl 2-13,08250 * 1,61233,000-16,3907-9,7743 STHJ 1750 /kg 6,30000 * 1,61233,001 2,9918 9,6082 STHJ 3500 /kg 1,52500 1,61233,353-1,7832 4,8332 STHJ 7000 /kg -12,02500 * 1,61233,000-15,3332-8,7168 STHT 1750 /kg 7,57500 * 1,61233,000 4,2668 10,8832 STHT 3500 /kg 3,20000 1,61233,057 -,1082 6,5082 Homogeneous Subsets Waktu_Mulai_Diare Percobaan Subset for alpha = 0.05 N 1 2 3 4 5 Duncan a oleum ricini 4 35,7250 CMC 1% 4 36,3000 STHT 1750 /kg 4 41,3250 STHJ 1750 /kg 4 42,6000 42,6000 STHT 3500 /kg 4 45,7000 45,7000 STHJ 3500 /kg 4 47,3750 STHT 7000 /kg 4 48,9000 STHJ 7000 /kg 4 60,9250 Suspensi Loperamid HCl 2 4 61,9825 Sig.,724,436,065,070,517 Means for groups in homogeneous subsets are displayed. a. Uses Harmonic Mean Sample Size = 4,000. 52

Lampiran 11. Data Analisis Statistik Frekuensi Diare Oneway Descriptives Frekuensi_diare 95% Confidence Interval for Std. Mean Maxim N Mean Deviation Std. Error Lower Bound Upper Bound Minimum um oleum ricini 4 11,7500 2,21736 1,10868 8,2217 15,2783 9,00 14,00 CMC 1% 4 11,5000 2,38048 1,19024 7,7121 15,2879 9,00 14,00 Suspensi Loperamid 4 5,5000 1,29099,64550 3,4457 7,5543 4,00 7,00 HCl 2 STHJ 1750 /kg 4 7,2500 1,70783,85391 4,5325 9,9675 5,00 9,00 STHJ 3500 /kg 4 6,5000 1,29099,64550 4,4457 8,5543 5,00 8,00 STHJ 7000 /kg 4 5,7500,95743,47871 4,2265 7,2735 5,00 7,00 STHT 1750 /kg 4 8,0000 1,63299,81650 5,4015 10,5985 6,00 10,00 STHT 3500 /kg 4 7,5000 1,73205,86603 4,7439 10,2561 6,00 10,00 STHT 7000 /kg 4 6,2500,95743,47871 4,7265 7,7735 5,00 7,00 Total 36 7,7778 2,65234,44206 6,8804 8,6752 4,00 14,00 ANOVA Frekuensi_diare Sum of Squares df Mean Square F Sig. Between Groups 173,222 8 21,653 8,009,000 Within Groups 73,000 27 2,704 Total 246,222 35 53

Lampiran (lanjutan) Post Hoc Tests Multiple Comparisons Dependent Variable:Frekuensi_diare (I) Percobaan (J) Percobaan Mean 95% Confidence Interval Difference Std. Lower (I-J) Error Sig. Bound Upper Bound LSD oleum ricini CMC 1%,25000 1,16269,831-2,1356 2,6356 Susp. Loperamid HCl 6,25000 * 1,16269,000 3,8644 8,6356 2 STHJ 1750 /kg 4,50000 * 1,16269,001 2,1144 6,8856 STHJ 3500 /kg 5,25000 * 1,16269,000 2,8644 7,6356 STHJ 7000 /kg 6,00000 * 1,16269,000 3,6144 8,3856 STHT 1750 /kg 3,75000 * 1,16269,003 1,3644 6,1356 STHT 3500 /kg 4,25000 * 1,16269,001 1,8644 6,6356 STHT 7000 /kg 5,50000 * 1,16269,000 3,1144 7,8856 CMC 1% oleum ricini -,25000 1,16269,831-2,6356 2,1356 Susp. Loperamid HCl 6,00000 * 1,16269,000 3,6144 8,3856 2 STHJ 1750 /kg 4,25000 * 1,16269,001 1,8644 6,6356 STHJ 3500 /kg 5,00000 * 1,16269,000 2,6144 7,3856 STHJ 7000 /kg 5,75000 * 1,16269,000 3,3644 8,1356 STHT 1750 /kg 3,50000 * 1,16269,006 1,1144 5,8856 STHT 3500 /kg 4,00000 * 1,16269,002 1,6144 6,3856 STHT 7000 /kg 5,25000 * 1,16269,000 2,8644 7,6356 Susp.Loperamid HCl 2 oleum ricini -6,25000 * 1,16269,000-8,6356-3,8644 CMC 1% -6,00000 * 1,16269,000-8,3856-3,6144 STHJ 1750 /kg -1,75000 1,16269,144-4,1356,6356 STHJ 3500 /kg -1,00000 1,16269,397-3,3856 1,3856 STHJ 7000 /kg -,25000 1,16269,831-2,6356 2,1356 STHT 1750 /kg -2,50000 * 1,16269,041-4,8856 -,1144 STHT 3500 /kg -2,00000 1,16269,097-4,3856,3856 STHT 7000 /kg -,75000 1,16269,524-3,1356 1,6356 STHJ 1750 /kg oleum ricini -4,50000 * 1,16269,001-6,8856-2,1144 54

CMC 1% -4,25000 * 1,16269,001-6,6356-1,8644 Susp.Loperamid HCl 2 1,75000 1,16269,144 -,6356 4,1356 STHJ 3500 /kg,75000 1,16269,524-1,6356 3,1356 STHJ 7000 /kg 1,50000 1,16269,208 -,8856 3,8856 STHT 1750 /kg -,75000 1,16269,524-3,1356 1,6356 STHT 3500 /kg -,25000 1,16269,831-2,6356 2,1356 STHT 7000 /kg 1,00000 1,16269,397-1,3856 3,3856 STHJ 3500 /kg oleum ricini -5,25000 * 1,16269,000-7,6356-2,8644 CMC 1% -5,00000 * 1,16269,000-7,3856-2,6144 Susp.Loperamid HCl 2 1,00000 1,16269,397-1,3856 3,3856 STHJ 1750 /kg -,75000 1,16269,524-3,1356 1,6356 STHJ 7000 /kg,75000 1,16269,524-1,6356 3,1356 STHT 1750 /kg -1,50000 1,16269,208-3,8856,8856 STHT 3500 /kg -1,00000 1,16269,397-3,3856 1,3856 STHT 7000 /kg,25000 1,16269,831-2,1356 2,6356 STHJ 7000 /kg oleum ricini -6,00000 * 1,16269,000-8,3856-3,6144 CMC 1% -5,75000 * 1,16269,000-8,1356-3,3644 Susp.Loperamid HCl 2,25000 1,16269,831-2,1356 2,6356 STHJ 1750 /kg -1,50000 1,16269,208-3,8856,8856 STHJ 3500 /kg -,75000 1,16269,524-3,1356 1,6356 STHT 1750 /kg -2,25000 1,16269,064-4,6356,1356 STHT 3500 /kg -1,75000 1,16269,144-4,1356,6356 STHT 7000 /kg -,50000 1,16269,671-2,8856 1,8856 STHT 1750 /kg oleum ricini -3,75000 * 1,16269,003-6,1356-1,3644 CMC 1% -3,50000 * 1,16269,006-5,8856-1,1144 Susp.Loperamid HCl 2 2,50000 * 1,16269,041,1144 4,8856 STHJ 1750 /kg,75000 1,16269,524-1,6356 3,1356 STHJ 3500 /kg 1,50000 1,16269,208 -,8856 3,8856 STHJ 7000 /kg 2,25000 1,16269,064 -,1356 4,6356 STHT 3500 /kg,50000 1,16269,671-1,8856 2,8856 STHT 7000 /kg 1,75000 1,16269,144 -,6356 4,1356 55

STHT 3500 /kg oleum ricini -4,25000 * 1,16269,001-6,6356-1,8644 CMC 1% -4,00000 * 1,16269,002-6,3856-1,6144 Susp.Loperamid HCl 2 2,00000 1,16269,097 -,3856 4,3856 STHJ 1750 /kg,25000 1,16269,831-2,1356 2,6356 STHJ 3500 /kg 1,00000 1,16269,397-1,3856 3,3856 STHJ 7000 /kg 1,75000 1,16269,144 -,6356 4,1356 STHT 1750 /kg -,50000 1,16269,671-2,8856 1,8856 STHT 7000 /kg 1,25000 1,16269,292-1,1356 3,6356 STHT 7000 /kg oleum ricini -5,50000 * 1,16269,000-7,8856-3,1144 CMC 1% -5,25000 * 1,16269,000-7,6356-2,8644 Susp. Loperamid HCl,75000 1,16269,524-1,6356 3,1356 2 STHJ 1750 /kg -1,00000 1,16269,397-3,3856 1,3856 STHJ 3500 /kg -,25000 1,16269,831-2,6356 2,1356 STHJ 7000 /kg,50000 1,16269,671-1,8856 2,8856 STHT 1750 /kg -1,75000 1,16269,144-4,1356,6356 STHT 3500 /kg -1,25000 1,16269,292-3,6356 1,1356 Homogeneous Subset Frekuensi_diare Percobaan Subset for alpha = 0.05 N 1 2 Duncan a Suspensi Loperamid HCl 2 4 5,5000 STHJ 7000 /kg 4 5,7500 STHT 7000 /kg 4 6,2500 STHJ 3500 /kg 4 6,5000 STHJ 1750 /kg 4 7,2500 STHT 3500 /kg 4 7,5000 STHT 1750 /kg 4 8,0000 CMC 1% 4 11,5000 oleum ricini 4 11,7500 Sig.,070,831 Means for groups in homogeneous subsets are displayed. 56

Lampiran 12. Data Analisis Statistik Bobot Feses ONEWAY Descriptives Bobot_feses 95% Confidence Interval for Mean Std. Lower Maximu N Mean Deviation Std. Error Bound Upper Bound Minimum m oleum ricini 4 1277,2500 181,78261 90,89130 987,9933 1566,5067 1014,00 1399,00 CMC 1% 4 1178,5000 71,72866 35,86433 1064,3637 1292,6363 1089,00 1245,00 Suspensi Loperamid 4 731,7500 154,48058 77,24029 485,9369 977,5631 544,00 883,00 HCl 2 STHJ 1750 /kg 4 934,2500 148,01661 74,00830 698,7225 1169,7775 715,00 1037,00 STHJ 3500 /kg 4 812,0000 104,38710 52,19355 645,8968 978,1032 749,00 968,00 STHJ 7000 /kg 4 745,2500 171,94064 85,97032 471,6541 1018,8459 550,00 966,00 STHT 1750 /kg 4 972,5000 210,41309 105,20654 637,6858 1307,3142 671,00 1161,00 STHT 3500 /kg 4 917,5000 173,19257 86,59629 641,9120 1193,0880 716,00 1090,00 STHT 7000 /kg 4 843,7500 98,09647 49,04823 687,6566 999,8434 710,00 940,00 Total 36 934,7500 223,00485 37,16748 859,2960 1010,2040 544,00 1399,00 ANOVA Bobot_feses Sum of Squares df Mean Square F Sig. Between Groups 1115644,000 8 139455,500 6,025,000 Within Groups 624946,750 27 23146,176 Total 1740590,750 35 57

Lampiran (lanjutan) Post Hoc Tests Multiple Comparisons Dependent Variable:Bobot_feses (I) Percobaan (J) Percobaan 95% Confidence Interval Mean Difference (I-J) Std. Error Sig. Lower Bound Upper Bound LSD oleum ricini CMC 1% 98,75000 107,57829,367-121,9824 319,4824 Susp.Loperamid HCl 2 545,50000 * 107,57829,000 324,7676 766,2324 STHJ 1750 /kg 343,00000 * 107,57829,004 122,2676 563,7324 STHJ 3500 /kg 465,25000 * 107,57829,000 244,5176 685,9824 STHJ 7000 /kg 532,00000 * 107,57829,000 311,2676 752,7324 STHT 1750 /kg 304,75000 * 107,57829,009 84,0176 525,4824 STHT 3500 /kg 359,75000 * 107,57829,002 139,0176 580,4824 STHT 7000 /kg 433,50000 * 107,57829,000 212,7676 654,2324 CMC 1% oleum ricini -98,75000 107,57829,367-319,4824 121,9824 Susp.Loperamid HCl 2 446,75000 * 107,57829,000 226,0176 667,4824 STHJ 1750 /kg 244,25000 * 107,57829,031 23,5176 464,9824 STHJ 3500 /kg 366,50000 * 107,57829,002 145,7676 587,2324 STHJ 7000 /kg 433,25000 * 107,57829,000 212,5176 653,9824 STHT 1750 /kg 206,00000 107,57829,066-14,7324 426,7324 STHT 3500 /kg 261,00000 * 107,57829,022 40,2676 481,7324 STHT 7000 /kg 334,75000 * 107,57829,004 114,0176 555,4824 Susp. Loperamid HCl 2 oleum ricini -545,50000 * 107,57829,000-766,2324-324,7676 CMC 1% -446,75000 * 107,57829,000-667,4824-226,0176 STHJ 1750 /kg -202,50000 107,57829,071-423,2324 18,2324 STHJ 3500 /kg -80,25000 107,57829,462-300,9824 140,4824 STHJ 7000 /kg -13,50000 107,57829,901-234,2324 207,2324 STHT 1750 /kg -240,75000 * 107,57829,034-461,4824-20,0176 STHT 3500 /kg -185,75000 107,57829,096-406,4824 34,9824 STHT 7000 /kg -112,00000 107,57829,307-332,7324 108,7324 STHJ 1750 /kg oleum ricini -343,00000 * 107,57829,004-563,7324-122,2676 58

CMC 1% -244,25000 * 107,57829,031-464,9824-23,5176 Susp.Loperamid HCl 2 202,50000 107,57829,071-18,2324 423,2324 STHJ 3500 /kg 122,25000 107,57829,266-98,4824 342,9824 STHJ 7000 /kg 189,00000 107,57829,090-31,7324 409,7324 STHT 1750 /kg -38,25000 107,57829,725-258,9824 182,4824 STHT 3500 /kg 16,75000 107,57829,877-203,9824 237,4824 STHT 7000 /kg 90,50000 107,57829,408-130,2324 311,2324 STHJ 3500 /kg oleum ricini -465,25000 * 107,57829,000-685,9824-244,5176 CMC 1% -366,50000 * 107,57829,002-587,2324-145,7676 Susp.Loperamid HCl 2 80,25000 107,57829,462-140,4824 300,9824 STHJ 1750 /kg -122,25000 107,57829,266-342,9824 98,4824 STHJ 7000 /kg 66,75000 107,57829,540-153,9824 287,4824 STHT 1750 /kg -160,50000 107,57829,147-381,2324 60,2324 STHT 3500 /kg -105,50000 107,57829,335-326,2324 115,2324 STHT 7000 /kg -31,75000 107,57829,770-252,4824 188,9824 STHJ 7000 /kg oleum ricini -532,00000 * 107,57829,000-752,7324-311,2676 CMC 1% -433,25000 * 107,57829,000-653,9824-212,5176 Susp.Loperamid HCl 2 13,50000 107,57829,901-207,2324 234,2324 STHJ 1750 /kg -189,00000 107,57829,090-409,7324 31,7324 STHJ 3500 /kg -66,75000 107,57829,540-287,4824 153,9824 STHT 1750 /kg -227,25000 * 107,57829,044-447,9824-6,5176 STHT 3500 /kg -172,25000 107,57829,121-392,9824 48,4824 STHT 7000 /kg -98,50000 107,57829,368-319,2324 122,2324 STHT 1750 /kg oleum ricini -304,75000 * 107,57829,009-525,4824-84,0176 CMC 1% -206,00000 107,57829,066-426,7324 14,7324 Susp.Loperamid HCl 2 240,75000 * 107,57829,034 20,0176 461,4824 STHJ 1750 /kg 38,25000 107,57829,725-182,4824 258,9824 STHJ 3500 /kg 160,50000 107,57829,147-60,2324 381,2324 STHJ 7000 /kg 227,25000 * 107,57829,044 6,5176 447,9824 STHT 3500 /kg 55,00000 107,57829,613-165,7324 275,7324 STHT 7000 /kg 128,75000 107,57829,242-91,9824 349,4824 STHT 3500 /kg oleum ricini -359,75000 * 107,57829,002-580,4824-139,0176 CMC 1% -261,00000 * 107,57829,022-481,7324-40,2676 59

Susp.Loperamid HCl 2 185,75000 107,57829,096-34,9824 406,4824 STHJ 1750 /kg -16,75000 107,57829,877-237,4824 203,9824 STHJ 3500 /kg 105,50000 107,57829,335-115,2324 326,2324 STHJ 7000 /kg 172,25000 107,57829,121-48,4824 392,9824 STHT 1750 /kg -55,00000 107,57829,613-275,7324 165,7324 STHT 7000 /kg 73,75000 107,57829,499-146,9824 294,4824 STHT 7000 /kg oleum ricini -433,50000 * 107,57829,000-654,2324-212,7676 CMC 1% -334,75000 * 107,57829,004-555,4824-114,0176 Susp.Loperamid HCl 2 112,00000 107,57829,307-108,7324 332,7324 STHJ 1750 /kg -90,50000 107,57829,408-311,2324 130,2324 STHJ 3500 /kg 31,75000 107,57829,770-188,9824 252,4824 STHJ 7000 /kg 98,50000 107,57829,368-122,2324 319,2324 STHT 1750 /kg -128,75000 107,57829,242-349,4824 91,9824 STHT 3500 /kg -73,75000 107,57829,499-294,4824 146,9824 Homogeneous Subsets Bobot_feses Percobaan Subset for alpha = 0.05 N 1 2 3 Duncan a Suspensi Loperamid HCl 2 4 731,7500 STHJ 7000 /kg 4 745,2500 STHJ 3500 /kg 4 812,0000 STHT 7000 /kg 4 843,7500 STHT 3500 /kg 4 917,5000 STHJ 1750 /kg 4 934,2500 STHT 1750 /kg 4 972,5000 972,5000 CMC 1% 4 1178,5000 1178,5000 oleum ricini 4 1277,2500 Sig.,060,066,367 Means for groups in homogeneous subsets are displayed. 60

Lampiran 13. Data Analisis Statistik Volume isi usus Oneway Descriptives Antisekretori_Volume_isi_usus 95% Confidence Interval for Std. Mean Minimu N Mean Deviation Std. Error Lower Bound Upper Bound m Maximum oleum ricini 4 487,5000 59,65177 29,82588 392,5807 582,4193 400,00 530,00 CMC 1% 4 485,0000 10,00000 5,00000 469,0878 500,9122 480,00 500,00 Suspensi Loperamid 4 335,2500 17,98842 8,99421 306,6264 363,8736 320,00 355,00 HCl 2 STHJ 1750 /kg 4 394,0000 18,54724 9,27362 364,4872 423,5128 376,00 410,00 STHJ 3500 /kg 4 368,2500 39,90301 19,95150 304,7554 431,7446 328,00 405,00 STHJ 7000 /kg 4 341,2500 34,24787 17,12394 286,7540 395,7460 315,00 390,00 STHT 1750 /kg 4 412,2500 39,07578 19,53789 350,0717 474,4283 359,00 450,00 STHT 3500 /kg 4 403,7500 32,12865 16,06432 352,6262 454,8738 378,00 450,00 STHT 7000 /kg 4 369,5000 21,62560 10,81280 335,0888 403,9112 350,00 400,00 Total 36 399,6389 60,71110 10,11852 379,0972 420,1806 315,00 530,00 ANOVA Antisekretori_Volume_isi_usus Sum of Squares df Mean Square F Sig. Between Groups 98650,556 8 12331,319 10,969,000 Within Groups 30353,750 27 1124,213 Total 129004,306 35 61

Lampiran (lanjutan) Post Hoc Tests Dependent Variable:Antisekretori_Volume_isi_usus Multiple Comparisons (I) Percobaan (J) Percobaan Mean 95% Confidence Interval Difference (I-J) Std. Error Sig. Lower Bound Upper Bound LSD oleum ricini CMC 1% 2,50000 23,70878,917-46,1464 51,1464 Susp.Loperamid HCl 2 152,25000 * 23,70878,000 103,6036 200,8964 STHJ 1750 /kg 93,50000 * 23,70878,001 44,8536 142,1464 STHJ 3500 /kg 119,25000 * 23,70878,000 70,6036 167,8964 STHJ 7000 /kg 146,25000 * 23,70878,000 97,6036 194,8964 STHT 1750 /kg 75,25000 * 23,70878,004 26,6036 123,8964 STHT 3500 /kg 83,75000 * 23,70878,002 35,1036 132,3964 STHT 7000 /kg 118,00000 * 23,70878,000 69,3536 166,6464 CMC 1% oleum ricini -2,50000 23,70878,917-51,1464 46,1464 Susp.Loperamid HCl 2 149,75000 * 23,70878,000 101,1036 198,3964 STHJ 1750 /kg 91,00000 * 23,70878,001 42,3536 139,6464 STHJ 3500 /kg 116,75000 * 23,70878,000 68,1036 165,3964 STHJ 7000 /kg 143,75000 * 23,70878,000 95,1036 192,3964 STHT 1750 /kg 72,75000 * 23,70878,005 24,1036 121,3964 STHT 3500 /kg 81,25000 * 23,70878,002 32,6036 129,8964 STHT 7000 /kg 115,50000 * 23,70878,000 66,8536 164,1464 Suspensi Loperamid HCl 2 oleum ricini -152,25000 * 23,70878,000-200,8964-103,6036 CMC 1% -149,75000 * 23,70878,000-198,3964-101,1036 STHJ 1750 /kg -58,75000 * 23,70878,020-107,3964-10,1036 STHJ 3500 /kg -33,00000 23,70878,175-81,6464 15,6464 STHJ 7000 /kg -6,00000 23,70878,802-54,6464 42,6464 STHT 1750 /kg -77,00000 * 23,70878,003-125,6464-28,3536 STHT 3500 /kg -68,50000 * 23,70878,008-117,1464-19,8536 STHT 7000 /kg -34,25000 23,70878,160-82,8964 14,3964 STHJ 1750 /kg oleum ricini -93,50000 * 23,70878,001-142,1464-44,8536 CMC 1% -91,00000 * 23,70878,001-139,6464-42,3536 Susp. Loperamid HCl 2 58,75000 * 23,70878,020 10,1036 107,3964 STHJ 3500 /kg 25,75000 23,70878,287-22,8964 74,3964 STHJ 7000 /kg 52,75000 * 23,70878,035 4,1036 101,3964 STHT 1750 /kg -18,25000 23,70878,448-66,8964 30,3964 62

STHT 3500 /kg -9,75000 23,70878,684-58,3964 38,8964 STHT 7000 /kg 24,50000 23,70878,311-24,1464 73,1464 STHJ 3500 /kg oleum ricini -119,25000 * 23,70878,000-167,8964-70,6036 CMC 1% -116,75000 * 23,70878,000-165,3964-68,1036 Susp.Loperamid HCl 2 33,00000 23,70878,175-15,6464 81,6464 STHJ 1750 /kg -25,75000 23,70878,287-74,3964 22,8964 STHJ 7000 /kg 27,00000 23,70878,265-21,6464 75,6464 STHT 1750 /kg -44,00000 23,70878,074-92,6464 4,6464 STHT 3500 /kg -35,50000 23,70878,146-84,1464 13,1464 STHT 7000 /kg -1,25000 23,70878,958-49,8964 47,3964 STHJ 7000 /kg oleum ricini -146,25000 * 23,70878,000-194,8964-97,6036 CMC 1% -143,75000 * 23,70878,000-192,3964-95,1036 Susp.Loperamid HCl 2 6,00000 23,70878,802-42,6464 54,6464 STHJ 1750 /kg -52,75000 * 23,70878,035-101,3964-4,1036 STHJ 3500 /kg -27,00000 23,70878,265-75,6464 21,6464 STHT 1750 /kg -71,00000 * 23,70878,006-119,6464-22,3536 STHT 3500 /kg -62,50000 * 23,70878,014-111,1464-13,8536 STHT 7000 /kg -28,25000 23,70878,244-76,8964 20,3964 STHT 1750 /kg oleum ricini -75,25000 * 23,70878,004-123,8964-26,6036 CMC 1% -72,75000 * 23,70878,005-121,3964-24,1036 Susp.Loperamid HCl 2 77,00000 * 23,70878,003 28,3536 125,6464 STHJ 1750 /kg 18,25000 23,70878,448-30,3964 66,8964 STHJ 3500 /kg 44,00000 23,70878,074-4,6464 92,6464 STHJ 7000 /kg 71,00000 * 23,70878,006 22,3536 119,6464 STHT 3500 /kg 8,50000 23,70878,723-40,1464 57,1464 STHT 7000 /kg 42,75000 23,70878,083-5,8964 91,3964 STHT 3500 /kg oleum ricini -83,75000 * 23,70878,002-132,3964-35,1036 CMC 1% -81,25000 * 23,70878,002-129,8964-32,6036 Susp.Loperamid HCl 2 68,50000 * 23,70878,008 19,8536 117,1464 STHJ 1750 /kg 9,75000 23,70878,684-38,8964 58,3964 STHJ 3500 /kg 35,50000 23,70878,146-13,1464 84,1464 STHJ 7000 /kg 62,50000 * 23,70878,014 13,8536 111,1464 STHT 1750 /kg -8,50000 23,70878,723-57,1464 40,1464 STHT 7000 /kg 34,25000 23,70878,160-14,3964 82,8964 STHT 7000 /kg oleum ricini -118,00000 * 23,70878,000-166,6464-69,3536 CMC 1% -115,50000 * 23,70878,000-164,1464-66,8536 Susp.Loperamid HCl 2 34,25000 23,70878,160-14,3964 82,8964 STHJ 1750 /kg -24,50000 23,70878,311-73,1464 24,1464 STHJ 3500 /kg 1,25000 23,70878,958-47,3964 49,8964 63

STHJ 7000 /kg 28,25000 23,70878,244-20,3964 76,8964 STHT 1750 /kg -42,75000 23,70878,083-91,3964 5,8964 STHT 3500 /kg -34,25000 23,70878,160-82,8964 14,3964 *. The mean difference is significant at the 0.05 level. Homogeneous Subsets Antisekretori_Volume_isi_usus Percobaan Subset for alpha = 0.05 N 1 2 3 Duncan a Suspensi Loperamid HCl 2 4 335,2500 STHJ 7000 /kg 4 341,2500 STHJ 3500 /kg 4 368,2500 368,2500 STHT 7000 /kg 4 369,5000 369,5000 STHJ 1750 /kg 4 394,0000 STHT 3500 /kg 4 403,7500 STHT 1750 /kg 4 412,2500 CMC 1% 4 485,0000 oleum ricini 4 487,5000 Sig.,198,107,917 64