Lampiran 1 Hasil persetujuan etik penelitian 46
Lampiran 2 Gambar tumbuhan Tumbuhan daun Afrika Daun Afrika 47
Lampiran 3Gambar alat, bahan, dan objek penelitian Alat Langendorff Injeksi Ketamin 48
Injeksi Heparin Injeksi Digoksin 49
Tikus sebelum dibedah 50
Lampiran 4 Bagan penelitian Ekstrak Etanol Daun Afrika Karakterisasi Skrining Fitokimia Uji Efek Inotropik dan Kronotropik PK air Alkaloid Flavonoid Glikosida Saponin Tanin Triterpenoid/Steroid Glikosida Jantung 51
Lampiran 5 Perhitungan penetapan kadar air EEDA Kadar air ekstrak = volume air berat sampel x 100% No Berat sampel (g) Volume awal (ml) Volume akhir (ml) 1 2,071 1,85 2,00 2 2,075 2,00 2,15 3 2,070 2,15 2,3 a. Berat sampel = 2,071g Volume air = 0,15 ml Kadar air = 0,15 2,071 b. Berat sampel = 2,075 g XX 100% = 7,243% Volume air = 0,15 ml Kadar air = 0,15 5 x 100% = 7,229% 2,07 c. Berat sampel = 2,070 g Volume air = 0,15 ml Kadar air = 0,15 2,075 x 100% = 7,246% Kadar air rata-rata = (7,243+7,229+7,246)% 3 = 7,239% 52
Lampiran 6 Data hasil uji statistik persen peningkatan kontraktilitas Descriptives 95% Confidence Interval for Mean Std. Std. Lower Upper N Mean Deviation Error Minimum Maximum EEDA 1 MG 3 54,67 21,032 12,143 2,42 106,91 33 75 EEDA 0,5 MG 3 21,67 9,452 5,457-1,81 45,15 11 29 EEDA 0,5 MG 3-2,67 10,116 5,840-27,80 22,46-9 9 EEDA 1 MG 3-7,00 17,776 10,263-51,16 37,16-21 13 digoksin 0,1 mg 3 90,67 17,926 10,349 46,14 135,20 70 102 digoksin 0,1 mg 3-13,67 2,082 1,202-18,84-8,50-16 -12 Total 18 23,94 40,580 9,565 3,76 44,12-21 102 ANOVA persen peningkatan kontraktilitas Sum of Squares Df Mean Square F Sig. Between Groups 25443,611 5 5088,722 23,934,000 Within Groups 2551,333 12 212,611 Total 27994,944 17 persen peningkatan kontraktilitas Tukey HSD Multiple Comparisons 95% Confidence Interval Mean Difference Std. Lower Upper (I) variasi dosis (J) variasi dosis (I-J) Error Sig. EEDA 1 MG EEDA 0,5 MG 33,000 11,905,131-6,99 72,99 57,333 * 11,905,004 17,34 97,32 53
61,667 * 11,905,002 21,68 101,66-36,000 11,905,087-75,99 3,99 68,333 * 11,905,001 28,34 108,32 EEDA 0,5 MG EEDA 1 MG -33,000 11,905,131-72,99 6,99 24,333 11,905,374-15,66 64,32 28,667 11,905,227-11,32 68,66-69,000 * 11,905,001-108,99-29,01 35,333 11,905,095-4,66 75,32 EEDA 1 MG -57,333 * 11,905,004-97,32-17,34 EEDA 0,5 MG -24,333 11,905,374-64,32 15,66 4,333 11,905,999-35,66 44,32-93,333 * 11,905,000-133,32-53,34 11,000 11,905,933-28,99 50,99 EEDA 1 MG -61,667 * 11,905,002-101,66-21,68 EEDA 0,5 MG -28,667 11,905,227-68,66 11,32-4,333 11,905,999-44,32 35,66-97,667 * 11,905,000-137,66-57,68 6,667 11,905,992-33,32 46,66 EEDA 1 MG 36,000 11,905,087-3,99 75,99 EEDA 0,5 MG 69,000 * 11,905,001 29,01 108,99 93,333 * 11,905,000 53,34 133,32 97,667 * 11,905,000 57,68 137,66 104,333 * 11,905,000 64,34 144,32 EEDA 1 MG -68,333 * 11,905,001-108,32-28,34 EEDA 0,5 MG -35,333 11,905,095-75,32 4,66-11,000 11,905,933-50,99 28,99 54
-6,667 11,905,992-46,66 33,32-104,333 * 11,905,000-144,32-64,34 *. The mean difference is significant at the 0.05 level. persen peningkatan denyut jantung Descriptives 95% Confidence Interval for Mean Std. Std. Lower Upper N Mean Deviation Error Minimum Maximum EEDA 1 MG 3 107,00 26,230 15,144 41,84 172,16 83 135 EEDA 0,5 MG 3 61,33 6,658 3,844 44,79 77,87 57 69 EEDA 0,5 MG 3-41,00 25,865 14,933-105,25 23,25-69 -18 EEDA 1 MG 3-24,00 64,094 37,005-183,22 135,22-62 50 Digoksin 0,1 3-79,33 18,502 10,682-125,30-33,37-98 -61 mg digoksin 0,1 mg 3-10,33 2,517 1,453-16,58-4,08-13 -8 Total 18 2,28 69,970 16,492-32,52 37,07-98 135 persen peningkatan denyut jantung ANOVA Sum of Squares Df Mean Square F Sig. Between Groups 71511,611 5 14302,322 14,649,000 Within Groups 11716,000 12 976,333 Total 83227,611 17 persen peningkatan denyut jantung Tukey HSD Multiple Comparisons 95% Confidence Interval Mean Difference Std. Lower Upper (I) variasi dosis (J) variasi dosis (I-J) Error Sig. 55
EEDA 1 MG EEDA 0,5 MG 45,667 25,513,506-40,03 131,36 148,000 * 25,513,001 62,31 233,69 131,000 * 25,513,003 45,31 216,69 Digoksin 0,1 mg 186,333 * 25,513,000 100,64 272,03 117,333 * 25,513,006 31,64 203,03 EEDA 0,5 MG EEDA 1 MG -45,667 25,513,506-131,36 40,03 102,333 * 25,513,017 16,64 188,03 85,333 25,513,051 -,36 171,03 Digoksin 0,1 mg 140,667 * 25,513,001 54,97 226,36 71,667 25,513,123-14,03 157,36 EEDA 1 MG -148,000 * 25,513,001-233,69-62,31 EEDA 0,5 MG -102,333 * 25,513,017-188,03-16,64-17,000 25,513,983-102,69 68,69 Digoksin 0,1 mg 38,333 25,513,669-47,36 124,03-30,667 25,513,828-116,36 55,03 EEDA 1 MG -131,000 * 25,513,003-216,69-45,31 EEDA 0,5 MG -85,333 25,513,051-171,03,36 17,000 25,513,983-68,69 102,69 Digoksin 0,1 mg 55,333 25,513,318-30,36 141,03-13,667 25,513,993-99,36 72,03 Digoksin 0,1 mg EEDA 1 MG -186,333 * 25,513,000-272,03-100,64 EEDA 0,5 MG -140,667 * 25,513,001-226,36-54,97-38,333 25,513,669-124,03 47,36-55,333 25,513,318-141,03 30,36-69,000 25,513,145-154,69 16,69 EEDA 1 MG -117,333 * 25,513,006-203,03-31,64 56
EEDA 0,5 MG -71,667 25,513,123-157,36 14,03 30,667 25,513,828-55,03 116,36 13,667 25,513,993-72,03 99,36 Digoksin 0,1 mg 69,000 25,513,145-16,69 154,69 *. The mean difference is significant at the 0.05 level. 57