Rimpang Jahe. Serbuk. Maserat Disaring

Transkripsi

1 Lampiran. 1 Metode Pembuatan Ekstrak Air Jahe Rimpang Jahe Serbuk Dibersihkan Dicuci Dikering anginkan Ditimbang Dihaluskan dan diayak Ditimbang Dimasukan kedalam botol kaca bersih Dimaserasi ± 48 jam dengan pelarut air yang telah didestilasi Maserat Disaring Filtrat Residu Diwaterbath Ekstrak kental Fresh dryer Ekstrak kering Direndam dengan pelarut air yang telah didestilasi ± 1 hari dan diaduk Disaring Filtrat Residu

2 Lampiran 2. Tabel 6. Data Kadar MDA dalam Testis Mencit Jantan Dewasa (µm/ml) Ulangan 1 9,031 13,849 15,439 12,045 6,482 6, ,442 11,614 11,366 16,896 9,429 8, ,230 15,306 8,949 10,488 6,978 16, ,515 14,570 17,690 9,611 7,409 9,131 x ±SD 9,305±2,429 13,835±1,595 13,361±3,937 12,260±3,250 7,575±1,293 10,240±4,066 Uji Normalitas MDA Testis, Uji Homogenitas MDA Testis, dan Oneway ANOVA MDA_testis Kelompok a. Lilliefors Significance Correction Hasil Uji: Data berdistribusi normal (p>0,05) Uji Homogenitas MDA Testis MDA_testis Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Tests of Normality Kolmogorov-Smirnov a Hasil Uji: Variansi Data Homogen normal (p>0,05) Shapiro-Wilk Statistic df Sig. Statistic df Sig Test of Homogeneity of Variance Levene Statistic df1 df2 Sig

3 Lanjutan Lampiran 2. Oneway ANOVA MDA_testis Sum of Squares df Mean Square F Sig. Between Groups Within Groups Total Hasil Uji: Tidak terdapat perbedaan yang nyata antar perlakuan p=0,051 (p>0,05)

4 Lampiran 3. Data Diameter Tubulus Seminiferus Testis. Tabel 7. Data Diameter Tubulus Seminiferus Testis. Ulangan 1 135,33 169,75 184,47 176,78 178,45 111, ,43 216,36 136,14 170,45 195,61 123, ,93 137,15 171,38 162,2 121,34 114, ,95 171,99 156,33 168,23 120,86 68,66 x ±SD 151,16±15,73 173,81±32,53 162,08±20,77169,42±6,02154,07±38,70104,68±24,57 Diameter_TS Kelompok Tests of Normality Tebal_TS Hasil Uji: Data berdistribusi normal (p>0,05) Diameter_TS Based on Mean Based on Median Based on Median and with adjusted df Kolmogorov-Smirnov a Statistic df Sig. Statistic df Sig Based on trimmed mean Hasil Tebal_TS Uji: Variansi Based Data on Mean Homogen normal (p>0,05) Shapiro-Wilk Test of Homogeneity of Variance Levene Statistic df1 df2 Sig

5 Lanjutan Lampiran 3. Spread vs. Level Plot of Tebal_TS by Kelompok Spread Level * Plot of LN of Spread vs LN of Level Slope = Power for transformation = Uji Normalitas dan Uji Homogenitas Diameter Tubulus Seminiferus Testis setelah data ditransformasi trans_tebal_ts Kelompok a. Lilliefors Significance Correction Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig Test of Homogeneity of Variance trans_tebal_ts Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Levene Statistic df1 df2 Sig

6 Lanjutan Lampiran 3. Oneway Diameter_TS Between Groups Within Groups Total Hasil: Tebal Sel Epitel Tubulus Seminiferus berbeda nyata antara masing-masing perlakuan. Jadi perlu dilakukan analisis lanjut dengan Analisis Post Hoc - Bonferroni. Post Hoc Tests ANOVA Sum of Squares df Mean Square F Sig

7 Lanjutan Lampiran 3. Multiple Comparisons Dependent Variable: Diameter_TS Bonferroni (I) Kelompok (J) Kelompok *. The mean difference is significant at the.05 level. Mean Difference 95% Confidence Interval (I-J) Std. Error Sig. Lower Bound Upper Bound * * * *

8 Lampiran 4. Data Tebal Epitel Tubulus Seminiferus Testis. Tabel 8. Data Tebal Lapisan Epitel Tubulus Seminiferus Testis. Ulangan 1 38,54 46,69 47,97 21,53 60,60 57, ,95 17,97 35,56 31,30 69,97 60, ,64 47,67 41,87 44,07 31,49 30, ,36 47,65 44,53 48,05 38,16 30,93 Rata2 39,37±4,66 40,00±14,69 42,48±5,25 36,24±12,13 50,06±18,20 44,90±16,21 Uji Normalitas dan Uji Homogenitas Tebal Spermatogenik dalam tubulus seminiferus. Diameter_TS Tebal_TS Kelompok a. Lilliefors Significance Correction Tests of Normality Kolmogorov-Smirnov a Shapiro-Wilk Statistic df Sig. Statistic df Sig Test of Homogeneity of Variance Diameter_TS Tebal_TS Based on Mean Based on Median Based on Median and with adjusted df Based on trimmed mean Levene Statistic df1 5 df2 18 Sig NPar Tests Kruskal-Wallis Test Ranks Tebal_TS Kelompok Total N Mean Rank

9 Lanjutan Lampiran 4. Test Statistics a,b Chi-Square df Asymp. Sig. Tebal_TS a. Kruskal Wallis Test b. Grouping Variable: Kelompok Hasil: Tebal Sel Epitel Tubulus Seminiferus berbeda tidak nyata antara masingmasing perlakuan. Jadi tidak perlu dilakukan analisis lanjut.

10 Lampiran 5. Konversi perhitungan dosis untuk berbagai jenis hewan dan manusia

11 Lampiran 6. Surat Rekomendasi Persetujuan Etik Penelitian Kesehatan

12 Lampiran 7. Hasil Identifikasi Tumbuhan

13 Lampiran 8. Hasil Skrining Fitokimia Senyawa Bahan Alam